BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3184927.2.1
(1157 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE060930.3|BE060930 HVSMEg0013N24f Hordeum vulgare pre-a... 139 2e-031
gb|BE456124.3|BE456124 HVSMEg0019N22f Hordeum vulgare pre-a... 139 2e-031
gb|BE455566.2|BE455566 HVSMEg0017N13f Hordeum vulgare pre-a... 84 1e-014
gb|BG343487.1|BG343487 HVSMEg0005O08f Hordeum vulgare pre-a... 42 0.040
>gb|BE060930.3|BE060930 HVSMEg0013N24f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0013N24f, mRNA sequence
Length = 896
Score = 139 bits (70), Expect = 2e-031
Identities = 202/246 (82%)
Strand = Plus / Minus
Query: 895 gatgcagtgtggaatacaccctcacaggccatcacggcgctgtcgaagctcccttcttcc 954
|||||||||||||| |||||||| || ||||||||||| |||||||||||||||||||
Sbjct: 271 gatgcagtgtggaagacaccctcgcaagccatcacggcatcgtcgaagctcccttcttcc 212
Query: 955 aacagatcagctcgcacgatttgcagcctctctttagcgccaggtaatttccaaaggtgg 1014
|| || ||||| | ||||| | ||||||||| || || |||| ||| ||||| |||||
Sbjct: 211 aagaggtcagccctcacgagctccagcctctccttggcaccagctaagttccagaggtgt 152
Query: 1015 gctgtcttttggtgatttcctgggtccctgacagtccctaccacatgatatccagactcg 1074
||| ||| | ||||| |||||||| ||||| ||||||| |||||||| || |||||
Sbjct: 151 gctaccttcttctgattgcctgggtctctgactgtccctagaacatgatacccggactca 92
Query: 1075 aggagccgtttgataagccaagaggcaacaaagcctgaggccccggttacgcataccttg 1134
|| |||||||||| |||||||||||| || || || ||||| || || || || ||||||
Sbjct: 91 agaagccgtttgacaagccaagaggcgacgaaaccagaggctccagtgacacacaccttg 32
Query: 1135 cccttg 1140
||||||
Sbjct: 31 cccttg 26
Score = 65.9 bits (33), Expect = 3e-009
Identities = 66/77 (85%)
Strand = Plus / Minus
Query: 760 gacgacgaagacgtaaggacgacccttttcaggaacgggttcttcttgcacgatctcagc 819
|||||||| ||||| ||||| ||||| || || || || ||||||||||| |||||||||
Sbjct: 400 gacgacgacgacgtgaggacaaccctcttgagaaatggattcttcttgcaggatctcagc 341
Query: 820 acgttcagagtaccgtt 836
|||||||| || |||||
Sbjct: 340 acgttcagggtgccgtt 324
>gb|BE456124.3|BE456124 HVSMEg0019N22f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0019N22f, mRNA sequence
Length = 873
Score = 139 bits (70), Expect = 2e-031
Identities = 202/246 (82%)
Strand = Plus / Minus
Query: 895 gatgcagtgtggaatacaccctcacaggccatcacggcgctgtcgaagctcccttcttcc 954
|||||||||||||| |||||||| || ||||||||||| |||||||||||||||||||
Sbjct: 272 gatgcagtgtggaagacaccctcgcaagccatcacggcatcgtcgaagctcccttcttcc 213
Query: 955 aacagatcagctcgcacgatttgcagcctctctttagcgccaggtaatttccaaaggtgg 1014
|| || ||||| | ||||| | ||||||||| || || |||| ||| ||||| |||||
Sbjct: 212 aagaggtcagccctcacgagctccagcctctccttggcaccagctaagttccagaggtgt 153
Query: 1015 gctgtcttttggtgatttcctgggtccctgacagtccctaccacatgatatccagactcg 1074
||| ||| | ||||| |||||||| ||||| ||||||| |||||||| || |||||
Sbjct: 152 gctaccttcttctgattgcctgggtctctgactgtccctagaacatgatacccggactca 93
Query: 1075 aggagccgtttgataagccaagaggcaacaaagcctgaggccccggttacgcataccttg 1134
|| |||||||||| |||||||||||| || || || ||||| || || || || ||||||
Sbjct: 92 agaagccgtttgacaagccaagaggcgacgaaaccagaggctccagtgacacacaccttg 33
Query: 1135 cccttg 1140
||||||
Sbjct: 32 cccttg 27
Score = 65.9 bits (33), Expect = 3e-009
Identities = 66/77 (85%)
Strand = Plus / Minus
Query: 760 gacgacgaagacgtaaggacgacccttttcaggaacgggttcttcttgcacgatctcagc 819
|||||||| ||||| ||||| ||||| || || || || ||||||||||| |||||||||
Sbjct: 401 gacgacgacgacgtgaggacaaccctcttgagaaatggattcttcttgcaggatctcagc 342
Query: 820 acgttcagagtaccgtt 836
|||||||| || |||||
Sbjct: 341 acgttcagggtgccgtt 325
Score = 56.0 bits (28), Expect = 3e-006
Identities = 49/56 (87%)
Strand = Plus / Minus
Query: 568 acgaacgacgggaggacagtcacaaggtcgatgccgttctccttggcgaactccca 623
||||||| ||| ||||| | ||| ||||||||| |||||||||||||||||||||
Sbjct: 593 acgaacgtcggaaggacggccacgaggtcgatgttgttctccttggcgaactccca 538
>gb|BE455566.2|BE455566 HVSMEg0017N13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017N13f, mRNA sequence
Length = 584
Score = 83.8 bits (42), Expect = 1e-014
Identities = 111/134 (82%)
Strand = Plus / Minus
Query: 337 cgtttcgcgagcgaggagaccagctcgtcgttgtccagcaccactgagctgcacaggtac 396
|||||||||||| ||| ||||||||||||| | |||| ||| | ||| |||| | ||||
Sbjct: 504 cgtttcgcgagcaaggcgaccagctcgtcgctacccagaaccgccgagttgcagatgtac 445
Query: 397 ctcccggcggcctggggggcctcgtacaccaggatgtggctgctcgcaacgtcgtcgatg 456
||||| ||| | | || ||||| ||||||||||||| |||||| ||||||||||||
Sbjct: 444 ctccccttggcgtccgcggtttcgtagaccaggatgtggcagctcgcgacgtcgtcgatg 385
Query: 457 tggacgtaccccat 470
||||||||||||||
Sbjct: 384 tggacgtaccccat 371
Score = 65.9 bits (33), Expect = 3e-009
Identities = 66/77 (85%)
Strand = Plus / Minus
Query: 760 gacgacgaagacgtaaggacgacccttttcaggaacgggttcttcttgcacgatctcagc 819
|||||||| ||||| ||||| ||||| || || || || ||||||||||| |||||||||
Sbjct: 81 gacgacgacgacgtgaggacaaccctcttgagaaatggattcttcttgcaggatctcagc 22
Query: 820 acgttcagagtaccgtt 836
|||||||| || |||||
Sbjct: 21 acgttcagggtgccgtt 5
Score = 56.0 bits (28), Expect = 3e-006
Identities = 49/56 (87%)
Strand = Plus / Minus
Query: 568 acgaacgacgggaggacagtcacaaggtcgatgccgttctccttggcgaactccca 623
||||||| ||| ||||| | ||| ||||||||| |||||||||||||||||||||
Sbjct: 273 acgaacgtcggaaggacggccacgaggtcgatgttgttctccttggcgaactccca 218
>gb|BG343487.1|BG343487 HVSMEg0005O08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005O08f, mRNA sequence
Length = 949
Score = 42.1 bits (21), Expect = 0.040
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 559 ggcccgatcacgaacgacggg 579
|||||||||||||||||||||
Sbjct: 595 ggcccgatcacgaacgacggg 575
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 94,083
Number of Sequences: 312970
Number of extensions: 94083
Number of successful extensions: 26297
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26283
Number of HSP's gapped (non-prelim): 14
length of query: 1157
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1138
effective length of database: 169,188,109
effective search space: 192536068042
effective search space used: 192536068042
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)