BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.3
(1548 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 78 1e-012
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 70 2e-010
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 70 2e-010
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 70 2e-010
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 70 2e-010
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 70 2e-010
gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow st... 64 1e-008
gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. v... 58 9e-007
gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke devel... 56 4e-006
gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedl... 46 0.003
gb|BE455856.2|BE455856 HVSMEg0015N01f Hordeum vulgare pre-a... 46 0.003
gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. v... 46 0.003
gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. v... 46 0.003
gb|CB874906.1|CB874906 HX06K19w HX Hordeum vulgare subsp. v... 46 0.003
gb|BF631326.2|BF631326 HVSMEb0015J22f Hordeum vulgare seedl... 44 0.014
gb|BQ470109.1|BQ470109 HX01M20T HX Hordeum vulgare subsp. v... 44 0.014
gb|BQ471104.1|BQ471104 HX04P20r HX Hordeum vulgare subsp. v... 44 0.014
gb|BM377171.2|BM377171 EBem05_SQ004_F09_R embryo, 14 DPA, n... 44 0.014
gb|AL506416.1|AL506416 AL506416 Hordeum vulgare Barke devel... 42 0.054
gb|AL507273.1|AL507273 AL507273 Hordeum vulgare Barke devel... 40 0.21
gb|BF627265.2|BF627265 HVSMEb0004G09f Hordeum vulgare seedl... 40 0.21
gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachi... 40 0.21
gb|BQ660177.1|BQ660177 HI01F04w HI Hordeum vulgare subsp. v... 40 0.21
gb|CB876272.1|CB876272 HX10M17w HX Hordeum vulgare subsp. v... 40 0.21
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 77.8 bits (39), Expect = 1e-012
Identities = 57/63 (90%)
Strand = Plus / Minus
Query: 680 cacgtccttctcgtccttgtagcgccccaggctgccgacgctgatgccgtggcctgggcc 739
||||||||||||||||||||| | ||| |||||||| | ||||||||||||||||||||
Sbjct: 74 cacgtccttctcgtccttgtacctcccgaggctgccaatactgatgccgtggcctgggcc 15
Query: 740 gca 742
|||
Sbjct: 14 gca 12
Score = 69.9 bits (35), Expect = 2e-010
Identities = 38/39 (97%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||||||||||||||||||| ||||||||||||||||||
Sbjct: 247 ttgttggggcagtacttctgatcgatgatgatggggtag 209
Score = 69.9 bits (35), Expect = 2e-010
Identities = 41/43 (95%)
Strand = Plus / Minus
Query: 600 gacttggagtcctcgtacgacttgatgcgcaggccgttcgtgg 642
||||||||||||||||||||||||||||| |||||||| ||||
Sbjct: 154 gacttggagtcctcgtacgacttgatgcggaggccgttggtgg 112
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 69.9 bits (35), Expect = 2e-010
Identities = 38/39 (97%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||||||||||||||||||| ||||||||||||||||||
Sbjct: 154 ttgttggggcagtacttctgatcgatgatgatggggtag 116
Score = 69.9 bits (35), Expect = 2e-010
Identities = 41/43 (95%)
Strand = Plus / Minus
Query: 600 gacttggagtcctcgtacgacttgatgcgcaggccgttcgtgg 642
||||||||||||||||||||||||||||| |||||||| ||||
Sbjct: 61 gacttggagtcctcgtacgacttgatgcggaggccgttggtgg 19
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 69.9 bits (35), Expect = 2e-010
Identities = 41/43 (95%)
Strand = Plus / Minus
Query: 600 gacttggagtcctcgtacgacttgatgcgcaggccgttcgtgg 642
||||||||||||||||||||||||||||| |||||||| ||||
Sbjct: 261 gacttggagtcctcgtacgacttgatgcggaggccgttggtgg 219
Score = 69.9 bits (35), Expect = 2e-010
Identities = 56/63 (88%)
Strand = Plus / Minus
Query: 680 cacgtccttctcgtccttgtagcgccccaggctgccgacgctgatgccgtggcctgggcc 739
||||||||||||||||||||| | ||| |||||||| | ||||||||||||| ||||||
Sbjct: 181 cacgtccttctcgtccttgtacctcccgaggctgccaatactgatgccgtggcttgggcc 122
Query: 740 gca 742
|||
Sbjct: 121 gca 119
Score = 69.9 bits (35), Expect = 2e-010
Identities = 74/87 (85%)
Strand = Plus / Minus
Query: 774 ccggggccgatggagatgcagtcgtcgccgacgccgatggtggtgctgctgatggtcacg 833
|||||||||||||||||||| |||||||| ||| ||||||||||| | ||||| | ||
Sbjct: 87 ccggggccgatggagatgcaatcgtcgccaacggcgatggtggtgtcgatgatgttgaca 28
Query: 834 ttgctggagtcgccgatgtggatgccg 860
||| |||||||| ||||||||||||
Sbjct: 27 ttggacgagtcgcccatgtggatgccg 1
Score = 61.9 bits (31), Expect = 6e-008
Identities = 37/39 (94%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||| ||||||||||||||| ||||||||||||||||||
Sbjct: 354 ttgtgggggcagtacttctgatcgatgatgatggggtag 316
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 69.9 bits (35), Expect = 2e-010
Identities = 38/39 (97%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||||||||||||||||||| ||||||||||||||||||
Sbjct: 50 ttgttggggcagtacttctgatcgatgatgatggggtag 12
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 69.9 bits (35), Expect = 2e-010
Identities = 38/39 (97%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||||||||||||||||||| ||||||||||||||||||
Sbjct: 129 ttgttggggcagtacttctgatcgatgatgatggggtag 91
Score = 63.9 bits (32), Expect = 1e-008
Identities = 35/36 (97%)
Strand = Plus / Minus
Query: 600 gacttggagtcctcgtacgacttgatgcgcaggccg 635
||||||||||||||||||||||||||||| ||||||
Sbjct: 36 gacttggagtcctcgtacgacttgatgcggaggccg 1
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 69.9 bits (35), Expect = 2e-010
Identities = 38/39 (97%)
Strand = Plus / Minus
Query: 507 ttgttggggcagtacttctggtcgatgatgatggggtag 545
|||||||||||||||||||| ||||||||||||||||||
Sbjct: 189 ttgttggggcagtacttctgatcgatgatgatggggtag 151
Score = 69.9 bits (35), Expect = 2e-010
Identities = 41/43 (95%)
Strand = Plus / Minus
Query: 600 gacttggagtcctcgtacgacttgatgcgcaggccgttcgtgg 642
||||||||||||||||||||||||||||| |||||||| ||||
Sbjct: 96 gacttggagtcctcgtacgacttgatgcggaggccgttggtgg 54
>gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_F12 5', mRNA sequence
Length = 311
Score = 63.9 bits (32), Expect = 1e-008
Identities = 41/44 (93%)
Strand = Plus / Minus
Query: 840 gagtcgccgatgtggatgccgtccgtgttggggctgtcgccggg 883
|||||||| |||||||||||||| |||||||||||||| |||||
Sbjct: 277 gagtcgcccatgtggatgccgtcggtgttggggctgtccccggg 234
Score = 42.1 bits (21), Expect = 0.054
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 918 ttgcactcgaagatgttgaggtggaagaacttg 950
||||||||| ||||||| | |||||||||||||
Sbjct: 199 ttgcactcgtagatgttcatgtggaagaacttg 167
>gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. vulgare cDNA clone HM10P12
3-PRIME, mRNA sequence
Length = 608
Score = 58.0 bits (29), Expect = 9e-007
Identities = 38/41 (92%)
Strand = Plus / Minus
Query: 703 gccccaggctgccgacgctgatgccgtggcctgggccgcac 743
||||||||||||||| ||||||||||||||| ||| |||||
Sbjct: 405 gccccaggctgccgatgctgatgccgtggcccgggtcgcac 365
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Minus
Query: 851 gtggatgccgtccgtgttggggctgtc 877
|||||||||||| ||||||||||||||
Sbjct: 257 gtggatgccgtcggtgttggggctgtc 231
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 578 ctggtcgatgatgatggggt 559
>gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C10T 5',
mRNA sequence
Length = 507
Score = 56.0 bits (28), Expect = 4e-006
Identities = 31/32 (96%)
Strand = Plus / Minus
Query: 703 gccccaggctgccgacgctgatgccgtggcct 734
||||||||||||||| ||||||||||||||||
Sbjct: 44 gccccaggctgccgatgctgatgccgtggcct 13
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 217 ctggtcgatgatgatggggt 198
>gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0019E22f, mRNA sequence
Length = 635
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Minus
Query: 851 gtggatgccgtccgtgttggggctgtc 877
|||||||||||| ||||||||||||||
Sbjct: 569 gtggatgccgtcggtgttggggctgtc 543
>gb|BE455856.2|BE455856 HVSMEg0015N01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0015N01f, mRNA sequence
Length = 576
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggtaggagac 550
|||||||||||||||||||| ||||||
Sbjct: 187 ctggtcgatgatgatggggttggagac 161
>gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D02
5-PRIME, mRNA sequence
Length = 650
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggtaggagac 550
|||||||||||||||||||| ||||||
Sbjct: 511 ctggtcgatgatgatggggttggagac 485
>gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. vulgare cDNA clone HX06K19
5-PRIME, mRNA sequence
Length = 546
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggtaggagac 550
|||||||||||||||||||| ||||||
Sbjct: 294 ctggtcgatgatgatggggttggagac 268
>gb|CB874906.1|CB874906 HX06K19w HX Hordeum vulgare subsp. vulgare cDNA clone HX06K19
3-PRIME, mRNA sequence
Length = 592
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 524 ctggtcgatgatgatggggtaggagac 550
|||||||||||||||||||| ||||||
Sbjct: 410 ctggtcgatgatgatggggttggagac 436
>gb|BF631326.2|BF631326 HVSMEb0015J22f Hordeum vulgare seedling shoot EST library HVcDNA0002
(Dehydration stress) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEb0015J22f, mRNA sequence
Length = 818
Score = 44.1 bits (22), Expect = 0.014
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 1071 ccctgcccgtcgatggtgccgt 1092
||||||||||||||||||||||
Sbjct: 561 ccctgcccgtcgatggtgccgt 540
>gb|BQ470109.1|BQ470109 HX01M20T HX Hordeum vulgare subsp. vulgare cDNA clone HX01M20
5-PRIME, mRNA sequence
Length = 462
Score = 44.1 bits (22), Expect = 0.014
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 1071 ccctgcccgtcgatggtgccgt 1092
||||||||||||||||||||||
Sbjct: 48 ccctgcccgtcgatggtgccgt 27
>gb|BQ471104.1|BQ471104 HX04P20r HX Hordeum vulgare subsp. vulgare cDNA clone HX04P20
5-PRIME, mRNA sequence
Length = 502
Score = 44.1 bits (22), Expect = 0.014
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 1071 ccctgcccgtcgatggtgccgt 1092
||||||||||||||||||||||
Sbjct: 48 ccctgcccgtcgatggtgccgt 27
>gb|BM377171.2|BM377171 EBem05_SQ004_F09_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ004_F09 5', mRNA sequence
Length = 401
Score = 44.1 bits (22), Expect = 0.014
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 1071 ccctgcccgtcgatggtgccgt 1092
||||||||||||||||||||||
Sbjct: 54 ccctgcccgtcgatggtgccgt 33
>gb|AL506416.1|AL506416 AL506416 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY03A18T 5',
mRNA sequence
Length = 677
Score = 42.1 bits (21), Expect = 0.054
Identities = 31/33 (93%), Gaps = 1/33 (3%)
Strand = Plus / Minus
Query: 703 gccccaggctgccgacg-ctgatgccgtggcct 734
||||||||||||||| | |||||||||||||||
Sbjct: 45 gccccaggctgccgatgcctgatgccgtggcct 13
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 218 ctggtcgatgatgatggggt 199
>gb|AL507273.1|AL507273 AL507273 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY01J01V 5',
mRNA sequence
Length = 470
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 219 ctggtcgatgatgatggggt 200
>gb|BF627265.2|BF627265 HVSMEb0004G09f Hordeum vulgare seedling shoot EST library HVcDNA0002
(Dehydration stress) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEb0004G09f, mRNA sequence
Length = 778
Score = 40.1 bits (20), Expect = 0.21
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 1326 tccgacttgccgtcgcccttggcg 1349
|||| |||||||||||||||||||
Sbjct: 175 tccggcttgccgtcgcccttggcg 152
>gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0008L17f, mRNA sequence
Length = 458
Score = 40.1 bits (20), Expect = 0.21
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 851 gtggatgccgtccgtgttggggct 874
|||||||||||| |||||||||||
Sbjct: 216 gtggatgccgtcggtgttggggct 193
>gb|BQ660177.1|BQ660177 HI01F04w HI Hordeum vulgare subsp. vulgare cDNA clone HI01F04
3-PRIME, mRNA sequence
Length = 618
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 479 ctggtcgatgatgatggggt 498
>gb|CB876272.1|CB876272 HX10M17w HX Hordeum vulgare subsp. vulgare cDNA clone HX10M17
3-PRIME, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 524 ctggtcgatgatgatggggt 543
||||||||||||||||||||
Sbjct: 478 ctggtcgatgatgatggggt 497
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 215,036
Number of Sequences: 312970
Number of extensions: 215036
Number of successful extensions: 59804
Number of sequences better than 0.5: 24
Number of HSP's better than 0.5 without gapping: 24
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 59734
Number of HSP's gapped (non-prelim): 68
length of query: 1548
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1529
effective length of database: 169,188,109
effective search space: 258688618661
effective search space used: 258688618661
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)