BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3092809.2.1
         (652 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BI960545.1|BI960545  HVSMEn0024P18f Hordeum vulgare rachi...    58   4e-007
gb|CB859379.1|CB859379  HI10E13w HI Hordeum vulgare subsp. v...    42   0.022
gb|BI956255.1|BI956255  HVSMEn0001K10f Hordeum vulgare rachi...    38   0.35 

>gb|BI960545.1|BI960545 HVSMEn0024P18f Hordeum vulgare rachis EST library HVcDNA0015
           (normal) Hordeum vulgare subsp. vulgare cDNA clone
           HVSMEn0024P18f, mRNA sequence
          Length = 658

 Score = 58.0 bits (29), Expect = 4e-007
 Identities = 37/40 (92%)
 Strand = Plus / Plus

                                                   
Query: 106 cgccgcggagctcggccgccacggggtgcgcgtcaactgc 145
           |||||||||||||||||||||||| || || |||||||||
Sbjct: 592 cgccgcggagctcggccgccacggcgtncgggtcaactgc 631



>gb|CB859379.1|CB859379 HI10E13w HI Hordeum vulgare subsp. vulgare cDNA clone HI10E13
           3-PRIME, mRNA sequence
          Length = 488

 Score = 42.1 bits (21), Expect = 0.022
 Identities = 24/25 (96%)
 Strand = Plus / Minus

                                    
Query: 307 cctggccagcgacgaggcgaggtac 331
           |||||||||||||||| ||||||||
Sbjct: 481 cctggccagcgacgagtcgaggtac 457



>gb|BI956255.1|BI956255 HVSMEn0001K10f Hordeum vulgare rachis EST library HVcDNA0015
           (normal) Hordeum vulgare subsp. vulgare cDNA clone
           HVSMEn0001K10f, mRNA sequence
          Length = 640

 Score = 38.2 bits (19), Expect = 0.35
 Identities = 19/19 (100%)
 Strand = Plus / Minus

                              
Query: 235 ctttgtcggcggcgaggcc 253
           |||||||||||||||||||
Sbjct: 250 ctttgtcggcggcgaggcc 232



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 88,493
Number of Sequences: 312970
Number of extensions: 88493
Number of successful extensions: 30517
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 30513
Number of HSP's gapped (non-prelim): 4
length of query: 652
length of database: 175,134,539
effective HSP length: 19
effective length of query: 633
effective length of database: 169,188,109
effective search space: 107096072997
effective search space used: 107096072997
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)