BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3023930.2.1
         (544 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BE420972.1|BE420972  HWM004.E01 ITEC HWM Barley Leaf Libr...   127   4e-028
gb|BE437755.1|BE437755  SFR007.B08F990617 ITEC SFR Barley Le...    38   0.29 
gb|AV836811.1|AV836811  AV836811 K. Sato unpublished cDNA li...    38   0.29 

>gb|BE420972.1|BE420972 HWM004.E01 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
           vulgare cDNA clone HWM004.E01, mRNA sequence
          Length = 1149

 Score =  127 bits (64), Expect = 4e-028
 Identities = 155/186 (83%)
 Strand = Plus / Plus

                                                                       
Query: 13  aactattggatgctacactttttcaaggaatctagcggcgccatcctccatcctacagcc 72
           ||||| |||||||||||||| |||||||| || |||||||| | | | || || ||| ||
Sbjct: 754 aactactggatgctacacttcttcaaggactcgagcggcgcaacctttcaccccacaacc 813

                                                                       
Query: 73  attcaggtctccagctacgatcagctggttgcgtctgccatcacttggcagaacgctaaa 132
           || ||| |||||| |||||||||| ||||||| ||||||||||| ||||||| | |||||
Sbjct: 814 atgcagatctccaactacgatcagatggttgcatctgccatcacctggcagagccctaaa 873

                                                                       
Query: 133 gacaagagcacttacctcaggataaaggtggtgaatttcggcaaccaagctgtggatctg 192
           ||||||||||||||||| |  |  ||||||||||| ||| |  | ||||| |||||||| 
Sbjct: 874 gacaagagcacttacctgaaaaataaggtggtgaacttccgnnagcaagccgtggatctc 933

                 
Query: 193 aacata 198
           ||||||
Sbjct: 934 aacata 939



>gb|BE437755.1|BE437755 SFR007.B08F990617 ITEC SFR Barley Leaf Epidermis Library Hordeum
           vulgare subsp. vulgare cDNA clone SF, mRNA sequence
          Length = 522

 Score = 38.2 bits (19), Expect = 0.29
 Identities = 28/31 (90%)
 Strand = Plus / Plus

                                          
Query: 277 gagaactccttccagcagccagagaaggtgg 307
           ||||| ||||||||||| ||||| |||||||
Sbjct: 196 gagaattccttccagcaaccagacaaggtgg 226



>gb|AV836811.1|AV836811 AV836811 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
           vulgare seedling leaves second leaf stage Hordeum
           vulgare subsp. vulgare cDNA clone basd3o13, mRNA
           sequence
          Length = 556

 Score = 38.2 bits (19), Expect = 0.29
 Identities = 28/31 (90%)
 Strand = Plus / Minus

                                          
Query: 277 gagaactccttccagcagccagagaaggtgg 307
           ||||| ||||||||||| ||||| |||||||
Sbjct: 313 gagaattccttccagcaaccagacaaggtgg 283



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 54,324
Number of Sequences: 312970
Number of extensions: 54324
Number of successful extensions: 15810
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15802
Number of HSP's gapped (non-prelim): 7
length of query: 544
length of database: 175,134,539
effective HSP length: 19
effective length of query: 525
effective length of database: 169,188,109
effective search space: 88823757225
effective search space used: 88823757225
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)