BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2969966.2.1
(1029 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. v... 430 e-119
emb|AJ234409.1|HVU234409 Hordeum vulgare partial mRNA; clon... 121 5e-026
gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. v... 70 2e-010
gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. v... 70 2e-010
gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedl... 46 0.002
gb|BF622633.1|BF622633 HVSMEa0007H08f Hordeum vulgare seedl... 40 0.14
>gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42F09
5-PRIME, mRNA sequence
Length = 576
Score = 430 bits (217), Expect = e-119
Identities = 477/563 (84%), Gaps = 3/563 (0%)
Strand = Plus / Plus
Query: 336 aggatgttatttgagactgaggaatcaagtgtccgcaggtacatggggacaataacagaa 395
|||||| | |||||||| |||||||| ||||| ||||||||||||||||| |||||||||
Sbjct: 1 aggatgctctttgagaccgaggaatctagtgttcgcaggtacatggggacgataacagaa 60
Query: 396 gtgagtgatgctgacccagtgcgttggcctagttcctattggagatcagtgaaggttggt 455
|| |||||||| || |||||||||||| |||||||||| |||||||| || |||||||||
Sbjct: 61 gtcagtgatgccgatccagtgcgttgggctagttcctactggagatcggtcaaggttggt 120
Query: 456 tgggatgaatcaaccgcaggagaaaggccacctagagtttctctgtgggaaattgagcca 515
|||||||||||||| || || ||||| || |||||||||||| | ||||| ||||| |||
Sbjct: 121 tgggatgaatcaactgctggggaaagaccgcctagagtttctttatgggagattgaacca 180
Query: 516 ctgacaacctttcctatgtatccatccctgttcccactgcgagttaagcatccttggtat 575
|||||||||||||||||||||| || |||| |||||| | ||||||||||| ||||||
Sbjct: 181 ttgacaacctttcctatgtatccctctttgtttccactgagggttaagcatccctggtat 240
Query: 576 tcaggacttgctgctcttcatgatgacagcaatgctttgatgtggctgagaggagtaacc 635
|| || | || | ||||| ||||||||||||||||||||||||||||||||||| |
Sbjct: 241 tctggtgtagcaggccttcaagatgacagcaatgctttgatgtggctgagaggagttgct 300
Query: 636 ggtgaagggggctttcattcttcgaacttccagtcacctggtataggttcctggggacaa 695
||||| || || | ||| ||| |||||| ||||||||||| || || ||||||||||||
Sbjct: 301 ggtgatggaggttatcaatctatgaactttcagtcacctggcattggctcctggggacaa 360
Query: 696 cagagactccatccatccttacttagcaatgaccatgatcagtatcaagcagtggt---t 752
||||| |||||||| || || || || | |||||||||||||| |||||||| || |
Sbjct: 361 cagaggctccatccttctttgctgagtagcgaccatgatcagtaccaagcagttgttgct 420
Query: 753 gctgccgcagctgcctcccagtccggtggttatctgaagcagcaattcctacaccttcaa 812
||||| || || |||||||| || ||||||||| |||| |||||||||||| |||||||
Sbjct: 421 gctgctgccgccgcctcccaatctggtggttatatgaaacagcaattcctaaaccttcag 480
Query: 813 caacctatgcagtccccacaggagcagtgcaatctcaatccgctgttgcagcaacaaata 872
|| ||||||||||| || || || || ||||| ||||| || |||||||||||||||||
Sbjct: 481 cagcctatgcagtcgcctcaagaacactgcaacctcaacccactgttgcagcaacaaatc 540
Query: 873 ctacagcaagcgagccaacagca 895
|| |||||||| |||||||||||
Sbjct: 541 ctgcagcaagcaagccaacagca 563
>emb|AJ234409.1|HVU234409 Hordeum vulgare partial mRNA; clone cMWG0660
Length = 485
Score = 121 bits (61), Expect = 5e-026
Identities = 109/125 (87%)
Strand = Plus / Plus
Query: 21 actggctggagtgtgtttgtcagtgctaagagacttgttgctggagattctgtgctattc 80
|||||||||||||| ||||| || || || ||||| ||||||||||| || |||||||||
Sbjct: 359 actggctggagtgtctttgttagcgccaaaagactcgttgctggagactcagtgctattc 418
Query: 81 atatggaatgagaaaaaccagcttttgcttggaatcagacacgctacccggccacagact 140
||||||||||||||||||||||||| ||| |||||||| | || | |||| ||||||||
Sbjct: 419 atatggaatgagaaaaaccagctttggctgggaatcaggcgtgccaaccggacacagact 478
Query: 141 gtgat 145
|||||
Sbjct: 479 gtgat 483
>gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. vulgare cDNA clone HV04L02
5-PRIME, mRNA sequence
Length = 566
Score = 69.9 bits (35), Expect = 2e-010
Identities = 89/107 (83%)
Strand = Plus / Plus
Query: 165 agtgacagcatgcacatcggactccttgcagcagcagctcatgctgctgcaacaaacagc 224
||||| |||||||| |||||| | ||||| || ||||||||||| || |||| ||||||
Sbjct: 175 agtgatagcatgcatatcggagttcttgctgctgcagctcatgcagcagcaaataacagc 234
Query: 225 cgcttcacaattttctataatcctagggcaagtccaacagaatttgt 271
| |||||||| |||||||| || ||||| ||||| ||||||||||
Sbjct: 235 caattcacaatattctataacccaagggccagtccttcagaatttgt 281
>gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. vulgare cDNA clone HU14H09
5-PRIME, mRNA sequence
Length = 628
Score = 69.9 bits (35), Expect = 2e-010
Identities = 89/107 (83%)
Strand = Plus / Plus
Query: 165 agtgacagcatgcacatcggactccttgcagcagcagctcatgctgctgcaacaaacagc 224
||||| |||||||| |||||| | ||||| || ||||||||||| || |||| ||||||
Sbjct: 175 agtgatagcatgcatatcggagttcttgctgctgcagctcatgcagcagcaaataacagc 234
Query: 225 cgcttcacaattttctataatcctagggcaagtccaacagaatttgt 271
| |||||||| |||||||| || ||||| ||||| ||||||||||
Sbjct: 235 caattcacaatattctataacccaagggccagtccttcagaatttgt 281
>gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011B10f, mRNA sequence
Length = 815
Score = 46.1 bits (23), Expect = 0.002
Identities = 56/67 (83%)
Strand = Plus / Plus
Query: 322 ttgggatgcgattcaggatgttatttgagactgaggaatcaagtgtccgcaggtacatgg 381
|||||||| | ||||||||| | || ||||||||||| || | ||||| ||||||||||
Sbjct: 60 ttgggatgaggttcaggatgctgttcgagactgaggattcgggggtccgaaggtacatgg 119
Query: 382 ggacaat 388
| |||||
Sbjct: 120 gcacaat 126
>gb|BF622633.1|BF622633 HVSMEa0007H08f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0007H08f, mRNA sequence
Length = 462
Score = 40.1 bits (20), Expect = 0.14
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1 tccaaagaattcggcacgacactg 24
||||||||||||||||||| ||||
Sbjct: 158 tccaaagaattcggcacgagactg 181
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 126,144
Number of Sequences: 312970
Number of extensions: 126144
Number of successful extensions: 42796
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 42781
Number of HSP's gapped (non-prelim): 11
length of query: 1029
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1010
effective length of database: 169,188,109
effective search space: 170879990090
effective search space used: 170879990090
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)