BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2751069.2.1
(940 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU996988.1|BU996988 HI06I11r HI Hordeum vulgare subsp. v... 212 2e-053
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 127 7e-028
gb|BF624205.2|BF624205 HVSMEa0012L04f Hordeum vulgare seedl... 56 2e-006
gb|BF258030.2|BF258030 HVSMEf0014I19f Hordeum vulgare seedl... 56 2e-006
gb|BE455905.3|BE455905 HVSMEg0017F08f Hordeum vulgare pre-a... 56 2e-006
gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-a... 40 0.13
>gb|BU996988.1|BU996988 HI06I11r HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
5-PRIME, mRNA sequence
Length = 656
Score = 212 bits (107), Expect = 2e-053
Identities = 212/247 (85%)
Strand = Plus / Minus
Query: 248 ccttcaaggcggacaacttcaagaatctcctcgttctccactgtgtgagcaagtggcatg 307
|||||||| || |||||||| ||||||||||| |||||||| ||||||||||||||||||
Sbjct: 458 ccttcaagacgaacaacttctagaatctcctcattctccaccgtgtgagcaagtggcatg 399
Query: 308 atcatctgccccccagaaaatctcatgggtcgcaacccaggaagtgagcacgcactggtc 367
|| ||||| | ||||||||||||||| || |||||||||||||||||||| | ||| |
Sbjct: 398 atgatctggctcccagaaaatctcatagggcgcaacccaggaagtgagcatgaacttgat 339
Query: 368 ttcaatgatgaagccaaatacgcatcaatattgtaatcagcccattcagatctatgctcc 427
|||| | ||||||| |||| ||||||| || ||||||||||||||||| | ||| |||
Sbjct: 338 ttcagcgctgaagccgaatatgcatcaaagttataatcagcccattcagaccgatgttcc 279
Query: 428 ctcagaaatcgtaccagtactgccggtgggacgctctgcagtaacatggatgccttcgca 487
|| |||||||| ||||||||||| ||||| || |||||||||||||| ||||| || |||
Sbjct: 278 cttagaaatcgaaccagtactgctggtggaacactctgcagtaacattgatgctttagca 219
Query: 488 caaataa 494
|| ||||
Sbjct: 218 catataa 212
Score = 127 bits (64), Expect = 7e-028
Identities = 136/160 (85%)
Strand = Plus / Minus
Query: 57 ctgttttcatatcaagtggtatgacacgaaagccagaggaaatcaatggagcatcatccg 116
|||||||||| || |||||||| ||||| ||||| || || || |||||||||||||| |
Sbjct: 643 ctgttttcatgtccagtggtataacacggaagcctgaagatattaatggagcatcatcag 584
Query: 117 gaaaatgctcatcaattggtgcaaacacaagctggaaggaggaacccacagatttctcat 176
| ||| ||||||||||||| ||||| ||||| |||||| |||| |||| |||||||| |
Sbjct: 583 ggaaaagctcatcaattggggcaaagacaagttggaagcaggaccccattgatttctcgt 524
Query: 177 ctattccagtgcaaagctggagaaggtggatgtcccttga 216
||||||||||||||| ||| || |||||||| || |||||
Sbjct: 523 ctattccagtgcaaaactgaagcaggtggatatctcttga 484
Score = 119 bits (60), Expect = 2e-025
Identities = 123/144 (85%)
Strand = Plus / Minus
Query: 552 ttgagttgcaagcaacaacaacgtcttcaatgccatctccccccattacagaccaaccat 611
||||||||||||| | || || ||||||||||||||||| ||||||||||||||||||
Sbjct: 157 ttgagttgcaagcgataatcacatcttcaatgccatctccggccattacagaccaaccat 98
Query: 612 catcattgaaaccactaatggcatcattaaaccccctgcttagtctttgactaaaggtcc 671
|||||||||| ||||| || ||||||||||| || || || || || |||||||| ||||
Sbjct: 97 catcattgaagccacttatagcatcattaaatcctctactcagcctctgactaaatgtcc 38
Query: 672 gtagaactgcaggttgccttccca 695
| ||||| ||||| ||||| ||||
Sbjct: 37 gcagaacagcaggctgcctcccca 14
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 5', mRNA sequence
Length = 633
Score = 127 bits (64), Expect = 7e-028
Identities = 136/160 (85%)
Strand = Plus / Minus
Query: 57 ctgttttcatatcaagtggtatgacacgaaagccagaggaaatcaatggagcatcatccg 116
|||||||||| || |||||||| ||||| ||||| || || || |||||||||||||| |
Sbjct: 217 ctgttttcatgtccagtggtataacacggaagcctgaagatattaatggagcatcatcag 158
Query: 117 gaaaatgctcatcaattggtgcaaacacaagctggaaggaggaacccacagatttctcat 176
| ||| ||||||||||||| ||||| ||||| |||||| |||| |||| |||||||| |
Sbjct: 157 ggaaaagctcatcaattggggcaaagacaagttggaagcaggaccccattgatttctcgt 98
Query: 177 ctattccagtgcaaagctggagaaggtggatgtcccttga 216
||||||||||||||| ||| || |||||||| || |||||
Sbjct: 97 ctattccagtgcaaaactgaagcaggtggatatctcttga 58
>gb|BF624205.2|BF624205 HVSMEa0012L04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012L04f, mRNA sequence
Length = 486
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 403 atcagcccattcagatctatgctccctcagaaatcgtaccagta 446
||||||||||||||| | ||||||||||||||| ||||| ||||
Sbjct: 328 atcagcccattcagagcgatgctccctcagaaaccgtactagta 285
>gb|BF258030.2|BF258030 HVSMEf0014I19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014I19f, mRNA sequence
Length = 563
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 403 atcagcccattcagatctatgctccctcagaaatcgtaccagta 446
||||||||||||||| | ||||||||||||||| ||||| ||||
Sbjct: 92 atcagcccattcagagcgatgctccctcagaaaccgtactagta 49
Score = 40.1 bits (20), Expect = 0.13
Identities = 41/48 (85%)
Strand = Plus / Minus
Query: 67 atcaagtggtatgacacgaaagccagaggaaatcaatggagcatcatc 114
|||||||||||| ||||| ||||| |||| | || ||||||||||||
Sbjct: 428 atcaagtggtatcacacggaagcccgagggtagcagtggagcatcatc 381
>gb|BE455905.3|BE455905 HVSMEg0017F08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017F08f, mRNA sequence
Length = 806
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 403 atcagcccattcagatctatgctccctcagaaatcgtaccagta 446
||||||||||||||| | ||||||||||||||| ||||| ||||
Sbjct: 420 atcagcccattcagagcgatgctccctcagaaaccgtactagta 377
>gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017A24f, mRNA sequence
Length = 905
Score = 40.1 bits (20), Expect = 0.13
Identities = 41/48 (85%)
Strand = Plus / Minus
Query: 67 atcaagtggtatgacacgaaagccagaggaaatcaatggagcatcatc 114
|||||||||||| ||||| ||||| |||| | || ||||||||||||
Sbjct: 203 atcaagtggtatcacacggaagcccgagggtagcagtggagcatcatc 156
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 86,286
Number of Sequences: 312970
Number of extensions: 86286
Number of successful extensions: 23756
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23741
Number of HSP's gapped (non-prelim): 14
length of query: 940
length of database: 175,134,539
effective HSP length: 19
effective length of query: 921
effective length of database: 169,188,109
effective search space: 155822248389
effective search space used: 155822248389
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)