BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750753.2.1
(1126 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA008952.1|CA008952 HU12K18r HU Hordeum vulgare subsp. v... 129 2e-028
gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA li... 125 3e-027
gb|CA030942.1|CA030942 HX08J07r HX Hordeum vulgare subsp. v... 125 3e-027
gb|BQ470466.1|BQ470466 HX03B03r HX Hordeum vulgare subsp. v... 123 1e-026
gb|CA024140.1|CA024140 HZ48H14r HZ Hordeum vulgare subsp. v... 86 3e-015
gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA li... 62 4e-008
gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA li... 54 1e-005
gb|AW983082.3|AW983082 HVSMEg0008A15f Hordeum vulgare pre-a... 46 0.003
gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. v... 46 0.003
gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. v... 46 0.003
>gb|CA008952.1|CA008952 HU12K18r HU Hordeum vulgare subsp. vulgare cDNA clone HU12K18
5-PRIME, mRNA sequence
Length = 609
Score = 129 bits (65), Expect = 2e-028
Identities = 349/441 (79%), Gaps = 2/441 (0%)
Strand = Plus / Plus
Query: 56 tccttctgcaagacattgacagcctctgataccagcactcatggtggattctcggtgctg 115
|||||||||||||| |||| || || || |||||||| ||||| || ||||| ||||||
Sbjct: 116 tccttctgcaagacgctgaccgcgtccgacaccagcacacatggcggcttctccgtgctg 175
Query: 116 cggcgccatgcagatgagtgtcttccgccactggatatgaccc-gtcaacctccaacaca 174
|| ||||| || |||||||| || || || ||||||||||| | ||| ||||| |||||
Sbjct: 176 cgccgccacgctgatgagtgcctccctcccctggatatgacgcagtct-cctcccacaca 234
Query: 175 agagcttgtagccaaggacctgcatggtgtggaatggcgctttcgccacatattcagagg 234
|||||| || || ||||| |||||||| |||| |||||||| |||||||| ||| | ||
Sbjct: 235 agagctcgtggcaaaggatctgcatggcatggagtggcgcttccgccacattttccgcgg 294
Query: 235 tcaaccacgaaggcatcttttgcagagtggctggagtgtttttgttagtgccaagcgtct 294
||||| | |||||||| | ||||| ||||||||||| ||||| ||| |||| | ||
Sbjct: 295 gcaacctaggaggcatctccttcagagcggctggagtgtctttgtcagttccaaaaggct 354
Query: 295 tggtgctggagatgccttcatctttctaagaggtgatagtggggagcttcgtgtaggagt 354
|| ||||| || |||||||| || || ||||| || || || ||||||||||| || ||
Sbjct: 355 tgtcgctggggacgccttcattttcctcagaggagagagcggtgagcttcgtgttggtgt 414
Query: 355 caggcgtgccatgaggcagcaagcaaatgttccatcttcagtaatatcaagccacagcat 414
||||| || ||||| |||| | || | | |||||||| ||||| || || || ||
Sbjct: 415 gaggcgggctatgagacagctttccaacatagcttcttcagtcatatctagtcatagtat 474
Query: 415 gcatcttggtgttcttgctacagcatggcatgctgttaacactgggaccatgtttactgt 474
||| ||||| || ||||| || |||||||||||| | ||||| |||||||| |||||
Sbjct: 475 gcaccttggagtccttgcaactgcatggcatgctatcaacacaaaaaccatgttcactgt 534
Query: 475 ctactacaagcctaggacaag 495
||||||||| || ||||||||
Sbjct: 535 ctactacaaacccaggacaag 555
>gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6b18 5', mRNA sequence
Length = 667
Score = 125 bits (63), Expect = 3e-027
Identities = 344/435 (79%), Gaps = 2/435 (0%)
Strand = Plus / Plus
Query: 56 tccttctgcaagacattgacagcctctgataccagcactcatggtggattctcggtgctg 115
|||||||||||||| |||| || || || |||||||| ||||| || ||||| ||||||
Sbjct: 182 tccttctgcaagaccctgacggcatccgacaccagcacgcatggcggcttctccgtgctg 241
Query: 116 cggcgccatgcagatgagtgtcttccgccactggatatgaccc-gtcaacctccaacaca 174
|| ||||| || || ||||| || || || ||||| ||||||| ||| || ||||||||
Sbjct: 242 cgccgccacgctgacgagtgcctccctcccctggacatgacccagtcgccc-ccaacaca 300
Query: 175 agagcttgtagccaaggacctgcatggtgtggaatggcgctttcgccacatattcagagg 234
||||||||| || ||||| |||||||| |||| |||||||| ||||| || ||| | ||
Sbjct: 301 agagcttgtggcaaaggatctgcatggcatggactggcgcttccgccatatcttccgtgg 360
Query: 235 tcaaccacgaaggcatcttttgcagagtggctggagtgtttttgttagtgccaagcgtct 294
||||| | |||||||| | ||||| || |||||||| ||||| ||| |||| | ||
Sbjct: 361 gcaacctaggaggcatctccttcagagcggttggagtgtgtttgtcagttccaaaaggct 420
Query: 295 tggtgctggagatgccttcatctttctaagaggtgatagtggggagcttcgtgtaggagt 354
|| ||||| ||||||||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 421 tgtagctggggatgccttcattttcctcagaggagagagtggcgagcttcgtgttggtgt 480
Query: 355 caggcgtgccatgaggcagcaagcaaatgttccatcttcagtaatatcaagccacagcat 414
||||| || ||||| |||| | || || || |||||||| || || || || |||||
Sbjct: 481 taggcgggctatgagacagctgtccaacgtgccttcttcagtcatttctagtcatagcat 540
Query: 415 gcatcttggtgttcttgctacagcatggcatgctgttaacactgggaccatgtttactgt 474
||||||||| || ||||| || |||||||| ||| | ||||| | |||||| || ||
Sbjct: 541 gcatcttggggtccttgcaactgcatggcacgctatcaacacgaaaagcatgttcacggt 600
Query: 475 ctactacaagcctag 489
||||||||| |||||
Sbjct: 601 ctactacaaacctag 615
>gb|CA030942.1|CA030942 HX08J07r HX Hordeum vulgare subsp. vulgare cDNA clone HX08J07
5-PRIME, mRNA sequence
Length = 622
Score = 125 bits (63), Expect = 3e-027
Identities = 344/435 (79%), Gaps = 2/435 (0%)
Strand = Plus / Plus
Query: 56 tccttctgcaagacattgacagcctctgataccagcactcatggtggattctcggtgctg 115
|||||||||||||| |||| || || || |||||||| ||||| || ||||| ||||||
Sbjct: 47 tccttctgcaagaccctgacggcatccgacaccagcacgcatggcggcttctccgtgctg 106
Query: 116 cggcgccatgcagatgagtgtcttccgccactggatatgaccc-gtcaacctccaacaca 174
|| ||||| || || ||||| || || || ||||| ||||||| ||| || ||||||||
Sbjct: 107 cgccgccacgctgacgagtgcctccctcccctggacatgacccagtcgccc-ccaacaca 165
Query: 175 agagcttgtagccaaggacctgcatggtgtggaatggcgctttcgccacatattcagagg 234
||||||||| || ||||| |||||||| |||| |||||||| ||||| || ||| | ||
Sbjct: 166 agagcttgtggcaaaggatctgcatggcatggactggcgcttccgccatatcttccgtgg 225
Query: 235 tcaaccacgaaggcatcttttgcagagtggctggagtgtttttgttagtgccaagcgtct 294
||||| | |||||||| | ||||| || |||||||| ||||| ||| |||| | ||
Sbjct: 226 gcaacctaggaggcatctccttcagagcggttggagtgtgtttgtcagttccaaaaggct 285
Query: 295 tggtgctggagatgccttcatctttctaagaggtgatagtggggagcttcgtgtaggagt 354
|| ||||| ||||||||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 286 tgtagctggggatgccttcattttcctcagaggagagagtggcgagcttcgtgttggtgt 345
Query: 355 caggcgtgccatgaggcagcaagcaaatgttccatcttcagtaatatcaagccacagcat 414
||||| || ||||| |||| | || || || |||||||| || || || || |||||
Sbjct: 346 taggcgggctatgagacagctgtccaacgtgccttcttcagtcatttctagtcatagcat 405
Query: 415 gcatcttggtgttcttgctacagcatggcatgctgttaacactgggaccatgtttactgt 474
||||||||| || ||||| || |||||||| ||| | ||||| | |||||| || ||
Sbjct: 406 gcatcttggggtccttgcaactgcatggcacgctatcaacacgaaaagcatgttcacggt 465
Query: 475 ctactacaagcctag 489
||||||||| |||||
Sbjct: 466 ctactacaaacctag 480
Score = 46.1 bits (23), Expect = 0.003
Identities = 51/59 (86%), Gaps = 1/59 (1%)
Strand = Plus / Plus
Query: 562 gggatgagatttaaaatgaggttt-aaggtgaagaggctccagaacaaaggttcactgg 619
||||||||||| | ||||||||| |||| |||||||| ||||| |||||||| |||||
Sbjct: 551 gggatgagattcaggatgaggtttgaaggcgaagaggcaccagagcaaaggtttactgg 609
>gb|BQ470466.1|BQ470466 HX03B03r HX Hordeum vulgare subsp. vulgare cDNA clone HX03B03
5-PRIME, mRNA sequence
Length = 632
Score = 123 bits (62), Expect = 1e-026
Identities = 310/390 (79%), Gaps = 2/390 (0%)
Strand = Plus / Plus
Query: 56 tccttctgcaagacattgacagcctctgataccagcactcatggtggattctcggtgctg 115
|||||||||||||| |||| || || || |||||||| ||||| || ||||| ||||||
Sbjct: 223 tccttctgcaagaccctgacggcatccgacaccagcacgcatggcggcttctccgtgctg 282
Query: 116 cggcgccatgcagatgagtgtcttccgccactggatatgaccc-gtcaacctccaacaca 174
|| ||||| || || ||||| || || || ||||| ||||||| ||| || ||||||||
Sbjct: 283 cgccgccacgctgacgagtgcctccctcccctggacatgacccagtcgccc-ccaacaca 341
Query: 175 agagcttgtagccaaggacctgcatggtgtggaatggcgctttcgccacatattcagagg 234
||||||||| || ||||| |||||||| |||| |||||||| ||||| || ||| | ||
Sbjct: 342 agagcttgtggcaaaggatctgcatggcatggactggcgcttccgccatatcttccgtgg 401
Query: 235 tcaaccacgaaggcatcttttgcagagtggctggagtgtttttgttagtgccaagcgtct 294
||||| | |||||||| | ||||| || |||||||| ||||| ||| |||| | ||
Sbjct: 402 gcaacctaggaggcatctccttcagagcggttggagtgtgtttgtcagttccaaaaggct 461
Query: 295 tggtgctggagatgccttcatctttctaagaggtgatagtggggagcttcgtgtaggagt 354
|| ||||| ||||||||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 462 tgtagctggggatgccttcattttcctcagaggagagagtggcgagcttcgtgttggtgt 521
Query: 355 caggcgtgccatgaggcagcaagcaaatgttccatcttcagtaatatcaagccacagcat 414
||||| || ||||| |||| | || || || |||||||| || || || || |||||
Sbjct: 522 taggcgggctatgagacagctgtccaacgtgccttcttcagtcatttctagtcatagcat 581
Query: 415 gcatcttggtgttcttgctacagcatggca 444
||||||||| || ||||| || ||||||||
Sbjct: 582 gcatcttggggtccttgcaactgcatggca 611
>gb|CA024140.1|CA024140 HZ48H14r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ48H14
5-PRIME, mRNA sequence
Length = 402
Score = 85.7 bits (43), Expect = 3e-015
Identities = 141/171 (82%), Gaps = 2/171 (1%)
Strand = Plus / Plus
Query: 56 tccttctgcaagacattgacagcctctgataccagcactcatggtggattctcggtgctg 115
|||||||||||||| |||| || || || |||||||| ||||| || ||||| ||||||
Sbjct: 231 tccttctgcaagacgctgaccgcgtccgacaccagcacacatggcggcttctccgtgctg 290
Query: 116 cggcgccatgcagatgagtgtcttccgccactggatatgac-ccgtcaacctccaacaca 174
|| ||||| || |||||||| || || || ||||||||||| | ||| ||||| |||||
Sbjct: 291 cgccgccacgctgatgagtgcctccctcccctggatatgacgcagtc-tcctcccacaca 349
Query: 175 agagcttgtagccaaggacctgcatggtgtggaatggcgctttcgccacat 225
|||||| || || ||||| |||||||| |||| |||||||| ||||||||
Sbjct: 350 agagctcgtggcaaaggatctgcatggcatggagtggcgcttccgccacat 400
>gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11l09 5', mRNA sequence
Length = 582
Score = 61.9 bits (31), Expect = 4e-008
Identities = 88/107 (82%)
Strand = Plus / Plus
Query: 389 tcttcagtaatatcaagccacagcatgcatcttggtgttcttgctacagcatggcatgct 448
|||||||| ||||| || || || ||||| ||||| || ||||| || ||||||||||||
Sbjct: 180 tcttcagtcatatctagtcatagtatgcaccttggagtccttgcaactgcatggcatgct 239
Query: 449 gttaacactgggaccatgtttactgtctactacaagcctaggacaag 495
| ||||| |||||||| |||||||||||||| || ||||||||
Sbjct: 240 atcaacacaaaaaccatgttcactgtctactacaaacccaggacaag 286
>gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33n21 5', mRNA sequence
Length = 684
Score = 54.0 bits (27), Expect = 1e-005
Identities = 69/83 (83%)
Strand = Plus / Plus
Query: 413 atgcatcttggtgttcttgctacagcatggcatgctgttaacactgggaccatgtttact 472
||||| ||||| || ||||| || |||||||||||| | ||||| |||||||| |||
Sbjct: 31 atgcaccttggagtccttgcaactgcatggcatgctatcaacacaaaaaccatgttcact 90
Query: 473 gtctactacaagcctaggacaag 495
||||||||||| || ||||||||
Sbjct: 91 gtctactacaaacccaggacaag 113
>gb|AW983082.3|AW983082 HVSMEg0008A15f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0008A15f, mRNA sequence
Length = 806
Score = 46.1 bits (23), Expect = 0.003
Identities = 51/59 (86%), Gaps = 1/59 (1%)
Strand = Plus / Plus
Query: 562 gggatgagatttaaaatgaggttt-aaggtgaagaggctccagaacaaaggttcactgg 619
||||||||||| || ||||||||| |||| |||||||| ||| | |||||||| |||||
Sbjct: 172 gggatgagattcaagatgaggtttgaaggcgaagaggcaccatagcaaaggtttactgg 230
Score = 40.1 bits (20), Expect = 0.15
Identities = 65/80 (81%)
Strand = Plus / Plus
Query: 410 agcatgcatcttggtgttcttgctacagcatggcatgctgttaacactgggaccatgttt 469
|||||||||||||| || ||||| || |||||||| ||| | ||||| | ||||||
Sbjct: 22 agcatgcatcttggggtccttgcaactgcatggcacgctatgaacacgaaaagcatgtta 81
Query: 470 actgtctactacaagcctag 489
|| ||||||||||| |||||
Sbjct: 82 acggtctactacaaacctag 101
>gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF15L16
5-PRIME, mRNA sequence
Length = 591
Score = 46.1 bits (23), Expect = 0.003
Identities = 51/59 (86%), Gaps = 1/59 (1%)
Strand = Plus / Plus
Query: 562 gggatgagatttaaaatgaggttt-aaggtgaagaggctccagaacaaaggttcactgg 619
||||||||||| | ||||||||| |||| |||||||| ||||| |||||||| |||||
Sbjct: 235 gggatgagattcaggatgaggtttgaaggcgaagaggcaccagagcaaaggtttactgg 293
Score = 42.1 bits (21), Expect = 0.039
Identities = 81/101 (80%)
Strand = Plus / Plus
Query: 389 tcttcagtaatatcaagccacagcatgcatcttggtgttcttgctacagcatggcatgct 448
|||||||| || || || || |||||||||||||| || ||||| || |||||||| |||
Sbjct: 64 tcttcagtcatttctagtcatagcatgcatcttggggtccttgcaactgcatggcacgct 123
Query: 449 gttaacactgggaccatgtttactgtctactacaagcctag 489
| ||||| | |||||| || ||||||||||| |||||
Sbjct: 124 atcaacacgaaaagcatgttcacggtctactacaaacctag 164
>gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. vulgare cDNA clone HX14E02
5-PRIME, mRNA sequence
Length = 655
Score = 46.1 bits (23), Expect = 0.003
Identities = 51/59 (86%), Gaps = 1/59 (1%)
Strand = Plus / Plus
Query: 562 gggatgagatttaaaatgaggttt-aaggtgaagaggctccagaacaaaggttcactgg 619
||||||||||| | ||||||||| |||| |||||||| ||||| |||||||| |||||
Sbjct: 234 gggatgagattcaggatgaggtttgaaggcgaagaggcaccagagcaaaggtttactgg 292
Score = 42.1 bits (21), Expect = 0.039
Identities = 81/101 (80%)
Strand = Plus / Plus
Query: 389 tcttcagtaatatcaagccacagcatgcatcttggtgttcttgctacagcatggcatgct 448
|||||||| || || || || |||||||||||||| || ||||| || |||||||| |||
Sbjct: 63 tcttcagtcatttctagtcatagcatgcatcttggggtccttgcaactgcatggcacgct 122
Query: 449 gttaacactgggaccatgtttactgtctactacaagcctag 489
| ||||| | |||||| || ||||||||||| |||||
Sbjct: 123 atcaacacgaaaagcatgttcacggtctactacaaacctag 163
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 102,952
Number of Sequences: 312970
Number of extensions: 102952
Number of successful extensions: 25184
Number of sequences better than 0.5: 10
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 25148
Number of HSP's gapped (non-prelim): 23
length of query: 1126
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1107
effective length of database: 169,188,109
effective search space: 187291236663
effective search space used: 187291236663
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)