BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750663.2.1
(1282 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ764079.1|BQ764079 EBro03_SQ006_P05_R root, 3 week, wat... 335 2e-090
gb|BQ763699.1|BQ763699 EBro03_SQ005_N01 _R root, 3 week, wa... 333 8e-090
gb|BM375019.2|BM375019 EBem06_SQ002_B16_R embryo, 21 DPA, n... 254 6e-066
gb|AL511223.1|AL511223 AL511223 Hordeum vulgare Barke devel... 40 0.18
>gb|BQ764079.1|BQ764079 EBro03_SQ006_P05_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_P05 5', mRNA sequence
Length = 717
Score = 335 bits (169), Expect = 2e-090
Identities = 402/479 (83%), Gaps = 3/479 (0%)
Strand = Plus / Minus
Query: 804 tcttgggaccacaaggcaaggtttctgacggagtcggacatcaagtccagggcacggaca 863
||||| |||||| |||||| || |||| || |||| |||||| |||||||| ||||
Sbjct: 483 tcttgcgaccaccgggcaagattagtgaccgaatcggccatcaaatccagggcgcggatg 424
Query: 864 gacctatcagctaaaccaactctagactcggcaaactgccgtgccgcatcgatgcagaag 923
||||| || | || |||||| || |||||||||||||| | | ||| || |||||||||
Sbjct: 423 gacctgtctgttagaccaaccctggactcggcaaactgtctcgacgcttcaatgcagaag 364
Query: 924 ccatagttcgctagtgaaaggtgagccccctctgtcatgatgaacacgatagcctcaaaa 983
| || || | | ||| |||||| ||||||| |||||| |||| ||||||||||||
Sbjct: 363 gcgtaatttgattttgagaggtga---ccctctgacatgataaacatgatagcctcaaag 307
Query: 984 ccgacttcggaagcatctggatggcgtgctgtaatggagagcagcaatattactgttctc 1043
|| ||| | ||||||||||| ||||| |||||||||||||||||||| | ||| ||||||
Sbjct: 306 cctactcctgaagcatctgggtggcgagctgtaatggagagcagcaacactacagttctc 247
Query: 1044 cagcccatctgtgactttatatgtgccgcatttgctttgacgagccgtgcaacctcctgt 1103
|||||||||||||| ||||| ||| |||||||||||||||| |||||||||||||| ||
Sbjct: 246 cagcccatctgtgattttatgtgtcccgcatttgctttgacaagccgtgcaacctcttgc 187
Query: 1104 gtgatgttctcacaatacgcatctgctacccgtgcgtcgagcttgagaactaactgcaac 1163
|| ||||||||||| || ||||||||||| ||||| || |||||||||||||| |||||
Sbjct: 186 gtaatgttctcacagtatgcatctgctacacgtgcatccagcttgagaactaattgcaat 127
Query: 1164 gacctcagtaattcatcagcaagattctccttgtatggtagcaacctctggcatatcctc 1223
|||||||| || || |||||||| ||||| |||||||| || |||||||||||||||| |
Sbjct: 126 gacctcagcaagtcgtcagcaaggttctctttgtatggaagtaacctctggcatatccgc 67
Query: 1224 aggagtccaaaaatagctttctccacgagagcacacggcataactgtggactgaactat 1282
|| ||||||||||||||||||||||| || || || ||||| || ||||||||||||||
Sbjct: 66 agaagtccaaaaatagctttctccacaagggcgcatggcatgaccgtggactgaactat 8
Score = 113 bits (57), Expect = 1e-023
Identities = 93/105 (88%)
Strand = Plus / Minus
Query: 650 tgttaggcatcgctggagagaagccagcgcatggtttcgcacctcctccctctgatccag 709
|||||||||||||||||| ||| ||| || ||||||||||||||||| ||||| ||||
Sbjct: 634 tgttaggcatcgctggagtgaaaccaatgcgtggtttcgcacctcctctctctggtccaa 575
Query: 710 gctcagtttcttcagtgcttgcagcagcctgagccacatctccct 754
||||||||| ||||| ||||||||||| ||||||||||||||||
Sbjct: 574 gctcagttttttcagcgcttgcagcagtttgagccacatctccct 530
>gb|BQ763699.1|BQ763699 EBro03_SQ005_N01 _R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_N01 5', mRNA sequence
Length = 546
Score = 333 bits (168), Expect = 8e-090
Identities = 291/332 (87%)
Strand = Plus / Minus
Query: 951 ccctctgtcatgatgaacacgatagcctcaaaaccgacttcggaagcatctggatggcgt 1010
||||||| |||||| |||| |||||||||||| || ||| | ||||||||||| |||||
Sbjct: 356 ccctctgacatgataaacatgatagcctcaaagcctactcctgaagcatctgggtggcga 297
Query: 1011 gctgtaatggagagcagcaatattactgttctccagcccatctgtgactttatatgtgcc 1070
|||||||||||||||||||| | ||| |||||||||||||||||||| ||||| ||| ||
Sbjct: 296 gctgtaatggagagcagcaacactacagttctccagcccatctgtgattttatgtgtccc 237
Query: 1071 gcatttgctttgacgagccgtgcaacctcctgtgtgatgttctcacaatacgcatctgct 1130
|||||||||||||| |||||||||||||| || || ||||||||||| || |||||||||
Sbjct: 236 gcatttgctttgacaagccgtgcaacctcttgcgtaatgttctcacagtatgcatctgct 177
Query: 1131 acccgtgcgtcgagcttgagaactaactgcaacgacctcagtaattcatcagcaagattc 1190
|| ||||| || |||||||||||||| ||||| |||||||| || || |||||||| |||
Sbjct: 176 acacgtgcatccagcttgagaactaattgcaatgacctcagcaagtcgtcagcaaggttc 117
Query: 1191 tccttgtatggtagcaacctctggcatatcctcaggagtccaaaaatagctttctccacg 1250
|| |||||||| || |||||||||||||||| ||| |||||||||||||||||||||||
Sbjct: 116 tctttgtatggaagtaacctctggcatatccgcagaagtccaaaaatagctttctccaca 57
Query: 1251 agagcacacggcataactgtggactgaactat 1282
|| || || ||||| || ||||||||||||||
Sbjct: 56 agggcgcatggcatgaccgtggactgaactat 25
>gb|BM375019.2|BM375019 EBem06_SQ002_B16_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_B16 5', mRNA sequence
Length = 551
Score = 254 bits (128), Expect = 6e-066
Identities = 308/368 (83%)
Strand = Plus / Minus
Query: 227 cgagatgttgtgcgcgtgaagccacgtcagctcccacaggctgtcgcccccgatcgtgct 286
||||||||||| | |||| ||||| |||||||||||||||||||||||||| ||||||||
Sbjct: 463 cgagatgttgttcacgtggagccaggtcagctcccacaggctgtcgccccctatcgtgct 404
Query: 287 cctcttcgcaaggatgcccttgctcttcatcacgagcagaatgttcttgagcaggtccgg 346
|||||| || ||||| ||| ||| ||||||| ||| ||| ||||| | || ||||||||
Sbjct: 403 cctcttggcgaggatacccctgcccttcatcgcgaccaggatgtttcttagaaggtccgg 344
Query: 347 gaccacctcctgcagccggtcgctccgcttgcctcggaccttgaccttgacgtacttctc 406
|| || |||||| ||| |||||| |||||||| ||||||||| ||||| | |||||||
Sbjct: 343 gatcagctcctgaagcttgtcgctgcgcttgccacggaccttggtcttgatgcacttctc 284
Query: 407 catccggctcaggacccccagccacagcttgcagaaactgcttaggccgaaaaggtccgg 466
||||| ||| |||||||||||||||||||||||||| ||||| || ||||| ||||| ||
Sbjct: 283 catcctgctgaggacccccagccacagcttgcagaagctgctcagcccgaagaggtctgg 224
Query: 467 caagagctggaggtataccttcgcaaccagcttcatggcgagcacaagtgacccttccat 526
|| ||||| ||||| |||| || ||||| |||| ||||||||| || || ||||||||
Sbjct: 223 taacagctgaaggtaggcctttgctaccagtttcacggcgagcacgagggatccttccat 164
Query: 527 gtttctgtagtccttctgggagtggttctggctgatctccagcaaatcatcgagcaagga 586
||| |||| ||| ||||| |||||||| ||| ||| |||||||| || || ||||| |
Sbjct: 163 gttcctgtggtctttctgtgagtggttttgggcgatttccagcaagtcgtccagcaacgc 104
Query: 587 gaatatga 594
||||||||
Sbjct: 103 gaatatga 96
>gb|AL511223.1|AL511223 AL511223 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07G05u 3',
mRNA sequence
Length = 497
Score = 40.1 bits (20), Expect = 0.18
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 460 ggtccggcaagagctggagg 479
||||||||||||||||||||
Sbjct: 493 ggtccggcaagagctggagg 474
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 121,165
Number of Sequences: 312970
Number of extensions: 121165
Number of successful extensions: 37019
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 37010
Number of HSP's gapped (non-prelim): 9
length of query: 1282
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1263
effective length of database: 169,188,109
effective search space: 213684581667
effective search space used: 213684581667
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)