BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2647492.2.1
(1179 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV833788.1|AV833788 AV833788 K. Sato unpublished cDNA li... 285 2e-075
gb|BU984150.1|BU984150 HF03C06r HF Hordeum vulgare subsp. v... 188 3e-046
gb|AV915847.1|AV915847 AV915847 K. Sato unpublished cDNA li... 168 3e-040
gb|CA002338.1|CA002338 HS07D05r HS Hordeum vulgare subsp. v... 168 3e-040
gb|AV922039.1|AV922039 AV922039 K. Sato unpublished cDNA li... 127 9e-028
gb|AV920960.1|AV920960 AV920960 K. Sato unpublished cDNA li... 98 8e-019
gb|CA000723.1|CA000723 HS07D05u HS Hordeum vulgare subsp. v... 88 8e-016
gb|CA016744.1|CA016744 HV06J06u HV Hordeum vulgare subsp. v... 78 7e-013
gb|BF266207.2|BF266207 HV_CEa0014H15f Hordeum vulgare seedl... 46 0.003
gb|AL505642.1|AL505642 AL505642 Hordeum vulgare Barke roots... 42 0.041
gb|AV914518.1|AV914518 AV914518 K. Sato unpublished cDNA li... 42 0.041
gb|BM817163.1|BM817163 HC109F12_T3.ab1 HC Hordeum vulgare s... 42 0.041
gb|AL504985.1|AL504985 AL504985 Hordeum vulgare Barke roots... 40 0.16
gb|BQ470109.1|BQ470109 HX01M20T HX Hordeum vulgare subsp. v... 40 0.16
gb|BQ471104.1|BQ471104 HX04P20r HX Hordeum vulgare subsp. v... 40 0.16
gb|BM377171.2|BM377171 EBem05_SQ004_F09_R embryo, 14 DPA, n... 40 0.16
gb|BI778119.2|BI778119 EBro07_SQ002_E13_R root, 3 week, red... 40 0.16
gb|CA007562.1|CA007562 HU08F12r HU Hordeum vulgare subsp. v... 40 0.16
gb|CA014919.1|CA014919 HT12L18r HT Hordeum vulgare subsp. v... 40 0.16
>gb|AV833788.1|AV833788 AV833788 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp. vulgare
cDNA clone bags19h20, mRNA sequence
Length = 617
Score = 285 bits (144), Expect = 2e-075
Identities = 281/327 (85%)
Strand = Plus / Minus
Query: 709 ctccacgccacctgatgtctcgcttccgaccgcgaagccagcaaacggggaggagcctgt 768
|||||| |||| ||| ||||| || ||||| | ||||||||||| ||||||||||| ||
Sbjct: 617 ctccacaccacntgaggtctcactcccgacagaaaagccagcaaaaggggaggagcccgt 558
Query: 769 gatccgccgcacggtgattccagaactggggcggccataggcgattccatactcatccca 828
|||||||| | |||||| | || ||||||||||||||||| || ||||| ||||||||
Sbjct: 557 gatccgcctaatggtgatgtctgagctggggcggccataggctatgccatattcatccca 498
Query: 829 gccactcttgatggcgatggaatcgtcaccagtcgaaatgtagcaatcctcgatgcagat 888
||||||||||||||||| || || || ||||| |||||||||||||| ||||| |
Sbjct: 497 tccactcttgatggcgatcaggtcatctcccgtcgagatgtagcaatcctcaatgcacac 438
Query: 889 gttgctgctggaatctggatcaattccatccgtgttgggggagtcatgtggagctaggat 948
||||||||| |||||||||||||||||||| |||||||| |||||||||||||| || ||
Sbjct: 437 gttgctgctcgaatctggatcaattccatcagtgttgggagagtcatgtggagccagtat 378
Query: 949 agtcacgtttctgatcacaacattgctgcaataaacaggatggatgttccagaatggcga 1008
||||| ||||||||||| ||||||||||||||||| |||||||||||||| ||||||||
Sbjct: 377 agtcaaatttctgatcaccacattgctgcaataaactggatggatgttccaaaatggcga 318
Query: 1009 atcctgaaagacgacattggagacaat 1035
|||||| || || | ||||||||||||
Sbjct: 317 atcctggaaaaccaaattggagacaat 291
Score = 46.1 bits (23), Expect = 0.003
Identities = 62/75 (82%)
Strand = Plus / Minus
Query: 1096 cttccacatatcccaccacgcgctgccttaaccaccaatagttccattctcgcctgtgat 1155
||||||||| ||||||||| | || |||| ||| | || || |||||||| ||||| ||
Sbjct: 230 cttccacatgtcccaccacacactaccttgcccatcgatggtgccattctcacctgtaat 171
Query: 1156 gacaacatcctgaag 1170
|| ||||||||||||
Sbjct: 170 gaaaacatcctgaag 156
>gb|BU984150.1|BU984150 HF03C06r HF Hordeum vulgare subsp. vulgare cDNA clone HF03C06
5-PRIME, mRNA sequence
Length = 593
Score = 188 bits (95), Expect = 3e-046
Identities = 257/311 (82%)
Strand = Plus / Minus
Query: 608 gagacagtgatgttcctgatgaagccgcccctgcctgtgttggtcttgacatggatccca 667
|||||||||||||| | ||||| || |||||||| | ||||||||||| || | |||
Sbjct: 325 gagacagtgatgtttcgaatgaaccctcccctgccggagttggtcttgatgtgaacacca 266
Query: 668 aaccctgagttgaagaagttcaagtgttctgcaaggacgttctccacgccacctgatgtc 727
||||||||| || |||| || | || || || ||||| |||||||| |||||||| |||
Sbjct: 265 aaccctgagctgtagaaatttagatgctcagcgaggacattctccacaccacctgaggtc 206
Query: 728 tcgcttccgaccgcgaagccagcaaacggggaggagcctgtgatccgccgcacggtgatt 787
|| || ||||| | ||||||||||| ||||||||||| |||||||||| | ||||||
Sbjct: 205 tcactcccgacagaaaagccagcaaaaggggaggagcccgtgatccgcctaatggtgatg 146
Query: 788 ccagaactggggcggccataggcgattccatactcatcccagccactcttgatggcgatg 847
| || ||||||||||||||||| || ||||| |||||||| |||||||||||||||||
Sbjct: 145 tctgagctggggcggccataggctatgccatattcatcccatccactcttgatggcgatc 86
Query: 848 gaatcgtcaccagtcgaaatgtagcaatcctcgatgcagatgttgctgctggaatctgga 907
|| || || ||||| |||||||||||||| ||||| | ||||||||| |||||||||
Sbjct: 85 aggtcatctcccgtcgagatgtagcaatcctcaatgcacacgttgctgctcgaatctgga 26
Query: 908 tcaattccatc 918
|||||||||||
Sbjct: 25 tcaattccatc 15
Score = 93.7 bits (47), Expect = 1e-017
Identities = 107/127 (84%)
Strand = Plus / Minus
Query: 410 gacaggcagatccgggagaaggccgagttggggatgcccttgatcagcccagcctccctg 469
||||| ||||| |||||||| |||||||| || |||||||||||||||||||||||| ||
Sbjct: 523 gacagacagatgcgggagaacgccgagtttggaatgcccttgatcagcccagcctccttg 464
Query: 470 acgttctggccctggacattctttaccgtaagggcgtcgacgatcgggagcgcactccgg 529
| |||||| || || |||||||||| | | ||| ||| ||||| ||||||||| | ||
Sbjct: 463 atgttctgcccttgaacattctttatcatcaggctgtccacgattgggagcgcattgtgg 404
Query: 530 ttgtagc 536
|||||||
Sbjct: 403 ttgtagc 397
>gb|AV915847.1|AV915847 AV915847 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16d24 5', mRNA sequence
Length = 625
Score = 168 bits (85), Expect = 3e-040
Identities = 226/273 (82%)
Strand = Plus / Minus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 441 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 382
Query: 324 gggacggctgcacgtcgagagcgccaccgctgacagcctcgcacttccacggccggacag 383
|||| |||||||||||||| || ||||| || ||||||| ||| ||||| || ||||| |
Sbjct: 381 gggatggctgcacgtcgagtgcaccaccactcacagcctggcatttccatgggcggactg 322
Query: 384 gcgcaccaccagtgaacttgacgttcgacaggcagatccgggagaaggccgagttgggga 443
|||| | ||| || ||| | ||| ||||| ||||| |||||||| |||||||| || |
Sbjct: 321 gcgcgctgccatggagcttaatgttagacagacagatgcgggagaacgccgagtttggaa 262
Query: 444 tgcccttgatcagcccagcctccctgacgttctggccctggacattctttaccgtaaggg 503
||||||||||||||||||||||| ||| |||||| || || |||||||||| | | |||
Sbjct: 261 tgcccttgatcagcccagcctccttgatgttctgcccttgaacattctttatcatcaggc 202
Query: 504 cgtcgacgatcgggagcgcactccggttgtagc 536
||| ||||| ||||||||| | |||||||||
Sbjct: 201 tgtccacgattgggagcgcattgtggttgtagc 169
>gb|CA002338.1|CA002338 HS07D05r HS Hordeum vulgare subsp. vulgare cDNA clone HS07D05
5-PRIME, mRNA sequence
Length = 546
Score = 168 bits (85), Expect = 3e-040
Identities = 226/273 (82%)
Strand = Plus / Minus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 396 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 337
Query: 324 gggacggctgcacgtcgagagcgccaccgctgacagcctcgcacttccacggccggacag 383
|||| |||||||||||||| || ||||| || ||||||| ||| ||||| || ||||| |
Sbjct: 336 gggatggctgcacgtcgagtgcaccaccactcacagcctggcatttccatgggcggactg 277
Query: 384 gcgcaccaccagtgaacttgacgttcgacaggcagatccgggagaaggccgagttgggga 443
|||| | ||| || ||| | ||| ||||| ||||| |||||||| |||||||| || |
Sbjct: 276 gcgcgctgccatggagcttaatgttagacagacagatgcgggagaacgccgagtttggaa 217
Query: 444 tgcccttgatcagcccagcctccctgacgttctggccctggacattctttaccgtaaggg 503
||||||||||||||||||||||| ||| |||||| || || |||||||||| | | |||
Sbjct: 216 tgcccttgatcagcccagcctccttgatgttctgcccttgaacattctttatcatcaggc 157
Query: 504 cgtcgacgatcgggagcgcactccggttgtagc 536
||| ||||| ||||||||| | |||||||||
Sbjct: 156 tgtccacgattgggagcgcattgtggttgtagc 124
>gb|AV922039.1|AV922039 AV922039 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19h20 3', mRNA sequence
Length = 657
Score = 127 bits (64), Expect = 9e-028
Identities = 160/192 (83%)
Strand = Plus / Plus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 466 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 525
Query: 324 gggacggctgcacgtcgagagcgccaccgctgacagcctcgcacttccacggccggacag 383
|||| |||||||||||||| || ||||| || ||||||| ||| ||||| || ||||| |
Sbjct: 526 gggatggctgcacgtcgagtgcaccaccactcacagcctggcatttccatgggcggactg 585
Query: 384 gcgcaccaccagtgaacttgacgttcgacaggcagatccgggagaaggccgagttgggga 443
|||| | ||| || ||| | ||| ||||| ||||| |||||||| |||||||| || |
Sbjct: 586 gcgcgctgccatggagcttaatgttagacagacagatgcgggagaacgccgagtttggaa 645
Query: 444 tgcccttgatca 455
||||||||||||
Sbjct: 646 tgcccttgatca 657
>gb|AV920960.1|AV920960 AV920960 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16d24 3', mRNA sequence
Length = 571
Score = 97.6 bits (49), Expect = 8e-019
Identities = 94/109 (86%)
Strand = Plus / Plus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 455 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 514
Query: 324 gggacggctgcacgtcgagagcgccaccgctgacagcctcgcacttcca 372
|||| |||||||||||||| || ||||| || ||||||| ||| |||||
Sbjct: 515 gggatggctgcacgtcgagtgcaccaccactcacagcctggcatttcca 563
>gb|CA000723.1|CA000723 HS07D05u HS Hordeum vulgare subsp. vulgare cDNA clone HS07D05
3-PRIME, mRNA sequence
Length = 573
Score = 87.7 bits (44), Expect = 8e-016
Identities = 77/88 (87%)
Strand = Plus / Plus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 480 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 539
Query: 324 gggacggctgcacgtcgagagcgccacc 351
|||| |||||||||||||| || |||||
Sbjct: 540 gggatggctgcacgtcgagtgcaccacc 567
>gb|CA016744.1|CA016744 HV06J06u HV Hordeum vulgare subsp. vulgare cDNA clone HV06J06
3-PRIME, mRNA sequence
Length = 551
Score = 77.8 bits (39), Expect = 7e-013
Identities = 66/75 (88%)
Strand = Plus / Plus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagacgtggaagtcagttctgtgcacg 323
||||||| || |||||||||||||||||| |||| ||| || ||||| |||||||||||
Sbjct: 477 agcatcagagggaatttgtacagaagctcatcccggacatgctagtcaattctgtgcacg 536
Query: 324 gggacggctgcacgt 338
|||| ||||||||||
Sbjct: 537 gggatggctgcacgt 551
>gb|BF266207.2|BF266207 HV_CEa0014H15f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0014H15f, mRNA sequence
Length = 540
Score = 46.1 bits (23), Expect = 0.003
Identities = 34/38 (89%)
Strand = Plus / Plus
Query: 264 agcatcaaagcgaatttgtacagaagctcgtcccagac 301
||||||| || |||||||||||||||||| |||| |||
Sbjct: 497 agcatcagagggaatttgtacagaagctcatcccngac 534
>gb|AL505642.1|AL505642 AL505642 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09B11V 5', mRNA sequence
Length = 500
Score = 42.1 bits (21), Expect = 0.041
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 973 gctgcaataaacaggatggatgttccagaatgg 1005
||||||||| ||||||||||| ||||| |||||
Sbjct: 121 gctgcaatatacaggatggatattccaaaatgg 89
>gb|AV914518.1|AV914518 AV914518 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags6l20 5', mRNA sequence
Length = 710
Score = 42.1 bits (21), Expect = 0.041
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 973 gctgcaataaacaggatggatgttccagaatgg 1005
||||||||| ||||||||||| ||||| |||||
Sbjct: 132 gctgcaatatacaggatggatattccaaaatgg 100
>gb|BM817163.1|BM817163 HC109F12_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC109F12_T3.ab1 similar to Polygalacturonase,, mRNA
sequence
Length = 864
Score = 42.1 bits (21), Expect = 0.041
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 973 gctgcaataaacaggatggatgttccagaatgg 1005
||||||||| ||||||||||| ||||| |||||
Sbjct: 438 gctgcaatatacaggatggatattccaaaatgg 406
>gb|AL504985.1|AL504985 AL504985 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07A11V 5', mRNA sequence
Length = 696
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 968 acattgctgcaataaacagg 987
||||||||||||||||||||
Sbjct: 160 acattgctgcaataaacagg 141
>gb|BQ470109.1|BQ470109 HX01M20T HX Hordeum vulgare subsp. vulgare cDNA clone HX01M20
5-PRIME, mRNA sequence
Length = 462
Score = 40.1 bits (20), Expect = 0.16
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 808 ggcgattccatactcatcccagccactcttgatggc 843
|||||| || ||||| |||||||| |||||||||||
Sbjct: 361 ggcgatgccgtactcgtcccagccgctcttgatggc 326
>gb|BQ471104.1|BQ471104 HX04P20r HX Hordeum vulgare subsp. vulgare cDNA clone HX04P20
5-PRIME, mRNA sequence
Length = 502
Score = 40.1 bits (20), Expect = 0.16
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 808 ggcgattccatactcatcccagccactcttgatggc 843
|||||| || ||||| |||||||| |||||||||||
Sbjct: 361 ggcgatgccgtactcgtcccagccgctcttgatggc 326
>gb|BM377171.2|BM377171 EBem05_SQ004_F09_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ004_F09 5', mRNA sequence
Length = 401
Score = 40.1 bits (20), Expect = 0.16
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 808 ggcgattccatactcatcccagccactcttgatggc 843
|||||| || ||||| |||||||| |||||||||||
Sbjct: 367 ggcgatgccgtactcgtcccagccgctcttgatggc 332
>gb|BI778119.2|BI778119 EBro07_SQ002_E13_R root, 3 week, reduced light, cv Optic, EBro07
Hordeum vulgare subsp. vulgare cDNA clone
EBro07_SQ002_E13 5', mRNA sequence
Length = 525
Score = 40.1 bits (20), Expect = 0.16
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 808 ggcgattccatactcatcccagccactcttgatggc 843
|||||| || ||||| |||||||| |||||||||||
Sbjct: 179 ggcgatgccgtactcgtcccagccgctcttgatggc 144
>gb|CA007562.1|CA007562 HU08F12r HU Hordeum vulgare subsp. vulgare cDNA clone HU08F12
5-PRIME, mRNA sequence
Length = 507
Score = 40.1 bits (20), Expect = 0.16
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 808 ggcgattccatactcatcccagccactcttgatggc 843
|||||| || ||||| |||||||| |||||||||||
Sbjct: 123 ggcgatgccgtactcgtcccagccgctcttgatggc 88
>gb|CA014919.1|CA014919 HT12L18r HT Hordeum vulgare subsp. vulgare cDNA clone HT12L18
5-PRIME, mRNA sequence
Length = 655
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 968 acattgctgcaataaacagg 987
||||||||||||||||||||
Sbjct: 506 acattgctgcaataaacagg 487
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 107,364
Number of Sequences: 312970
Number of extensions: 107364
Number of successful extensions: 27524
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 27485
Number of HSP's gapped (non-prelim): 32
length of query: 1179
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1160
effective length of database: 169,188,109
effective search space: 196258206440
effective search space used: 196258206440
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)