BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2622915.2.2
(562 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV064104.1|CV064104 BNEL98b1 Barley EST endosperm librar... 172 8e-042
gb|CA015797.1|CA015797 HV11O17u HV Hordeum vulgare subsp. v... 167 5e-040
gb|BU974893.1|BU974893 HB29G22r BC Hordeum vulgare subsp. v... 159 1e-037
gb|CB881163.1|CB881163 HM08P11w HM Hordeum vulgare subsp. v... 151 3e-035
gb|CB881416.1|CB881416 HM09L23w HM Hordeum vulgare subsp. v... 151 3e-035
gb|BU995027.1|BU995027 HM08P11r HM Hordeum vulgare subsp. v... 149 1e-034
gb|BU995276.1|BU995276 HM09L23r HM Hordeum vulgare subsp. v... 147 5e-034
gb|BM374601.1|BM374601 EBma05_SQ002_C03_R maternal, 12 DPA,... 113 6e-024
gb|BQ664756.1|BQ664756 HV04L17u HV Hordeum vulgare subsp. v... 111 2e-023
gb|BI780945.1|BI780945 EBma05_SQ001_J20_R maternal, 12 DPA,... 100 9e-020
gb|CA019419.1|CA019419 HV11O17r HV Hordeum vulgare subsp. v... 66 1e-009
gb|BU974803.1|BU974803 HB29C15r BC Hordeum vulgare subsp. v... 44 0.005
>gb|CV064104.1|CV064104 BNEL98b1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98b1 5' similar to putative
sterol 4-alpha-methyl-oxidase, mRNA sequence
Length = 640
Score = 172 bits (87), Expect = 8e-042
Identities = 161/186 (86%)
Strand = Plus / Plus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 365 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 424
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccgagcca 390
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||| |||||
Sbjct: 425 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagcct 484
Query: 391 ccgtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtgagcctcn 450
|| |||||||| ||||| |||||||||||||| || |||||||| || |||||||| ||
Sbjct: 485 ccatacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtgagcttcc 544
Query: 451 accgtc 456
||||||
Sbjct: 545 accgtc 550
>gb|CA015797.1|CA015797 HV11O17u HV Hordeum vulgare subsp. vulgare cDNA clone HV11O17
3-PRIME, mRNA sequence
Length = 536
Score = 167 bits (84), Expect = 5e-040
Identities = 153/176 (86%)
Strand = Plus / Plus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 357 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 416
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccgagcca 390
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||| |||||
Sbjct: 417 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagcct 476
Query: 391 ccgtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtgagc 446
|| |||||||| ||||| |||||||||||||| || |||||||| || ||||||||
Sbjct: 477 ccatacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtgagc 532
>gb|BU974893.1|BU974893 HB29G22r BC Hordeum vulgare subsp. vulgare cDNA clone HB29G22
5-PRIME, mRNA sequence
Length = 499
Score = 159 bits (80), Expect = 1e-037
Identities = 143/164 (87%)
Strand = Plus / Minus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 171 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 112
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccgagcca 390
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||| |||||
Sbjct: 111 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagcct 52
Query: 391 ccgtacagtggaaggaaatttgatgggctccatggaaagtgata 434
|| |||||||| ||||| |||||||||||||| || ||||||||
Sbjct: 51 ccatacagtggcaggaagtttgatgggctccaagggaagtgata 8
>gb|CB881163.1|CB881163 HM08P11w HM Hordeum vulgare subsp. vulgare cDNA clone HM08P11
3-PRIME, mRNA sequence
Length = 604
Score = 151 bits (76), Expect = 3e-035
Identities = 161/189 (85%), Gaps = 3/189 (1%)
Strand = Plus / Plus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 333 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 392
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccga---g 387
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||| || |
Sbjct: 393 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagctg 452
Query: 388 ccaccgtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtgagcc 447
|| || |||||||| ||||| |||||||||||||| || |||||||| || ||||||||
Sbjct: 453 cctccatacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtgagct 512
Query: 448 tcnaccgtc 456
|| ||||||
Sbjct: 513 tccaccgtc 521
>gb|CB881416.1|CB881416 HM09L23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09L23
3-PRIME, mRNA sequence
Length = 576
Score = 151 bits (76), Expect = 3e-035
Identities = 161/189 (85%), Gaps = 3/189 (1%)
Strand = Plus / Plus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 332 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 391
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccga---g 387
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||| || |
Sbjct: 392 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagctg 451
Query: 388 ccaccgtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtgagcc 447
|| || |||||||| ||||| |||||||||||||| || |||||||| || ||||||||
Sbjct: 452 cctccatacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtgagct 511
Query: 448 tcnaccgtc 456
|| ||||||
Sbjct: 512 tccaccgtc 520
>gb|BU995027.1|BU995027 HM08P11r HM Hordeum vulgare subsp. vulgare cDNA clone HM08P11
5-PRIME, mRNA sequence
Length = 621
Score = 149 bits (75), Expect = 1e-034
Identities = 157/184 (85%), Gaps = 3/184 (1%)
Strand = Plus / Minus
Query: 276 cttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgaggcata 335
|||||||||||| || || || | ||||||||||||||||||||||| || ||||| ||
Sbjct: 621 cttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagaggcgta 562
Query: 336 gttccctgactttgtgtacagcactcggtgatggtagtcatggaagtccga---gccacc 392
|||||||||||| ||||| ||||| || |||||||||||||||||||| || ||| ||
Sbjct: 561 gttccctgacttggtgtagagcacacgatgatggtagtcatggaagtcagagctgcctcc 502
Query: 393 gtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtgagcctcnac 452
|||||||| ||||| |||||||||||||| || |||||||| || |||||||| || ||
Sbjct: 501 atacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtgagcttccac 442
Query: 453 cgtc 456
||||
Sbjct: 441 cgtc 438
>gb|BU995276.1|BU995276 HM09L23r HM Hordeum vulgare subsp. vulgare cDNA clone HM09L23
5-PRIME, mRNA sequence
Length = 591
Score = 147 bits (74), Expect = 5e-034
Identities = 138/159 (86%), Gaps = 3/159 (1%)
Strand = Plus / Minus
Query: 301 aacaaccagtccatgtaaacaaatgtcgaggcatagttccctgactttgtgtacagcact 360
||||||||||||||||||||||| || ||||| |||||||||||||| ||||| |||||
Sbjct: 569 aacaaccagtccatgtaaacaaaagtagaggcgtagttccctgacttggtgtagagcaca 510
Query: 361 cggtgatggtagtcatggaagtccga---gccaccgtacagtggaaggaaatttgatggg 417
|| |||||||||||||||||||| || ||| || |||||||| ||||| |||||||||
Sbjct: 509 cgatgatggtagtcatggaagtcagagctgcctccatacagtggcaggaagtttgatggg 450
Query: 418 ctccatggaaagtgatagccgctgtgagcctcnaccgtc 456
||||| || |||||||| || |||||||| || ||||||
Sbjct: 449 ctccaagggaagtgatatccactgtgagcttccaccgtc 411
>gb|BM374601.1|BM374601 EBma05_SQ002_C03_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ002_C03 5', mRNA sequence
Length = 398
Score = 113 bits (57), Expect = 6e-024
Identities = 99/113 (87%)
Strand = Plus / Minus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 113 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 54
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagtc 383
|| |||||||||||||| ||||| ||||| || ||||||||||||||||||||
Sbjct: 53 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagtc 1
>gb|BQ664756.1|BQ664756 HV04L17u HV Hordeum vulgare subsp. vulgare cDNA clone HV04L17
3-PRIME, mRNA sequence
Length = 615
Score = 111 bits (56), Expect = 2e-023
Identities = 98/112 (87%)
Strand = Plus / Plus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 504 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 563
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcatggaagt 382
|| |||||||||||||| ||||| ||||| || |||||||||||||||||||
Sbjct: 564 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcatggaagt 615
>gb|BI780945.1|BI780945 EBma05_SQ001_J20_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_J20 5', mRNA sequence
Length = 398
Score = 99.6 bits (50), Expect = 9e-020
Identities = 92/106 (86%)
Strand = Plus / Minus
Query: 271 tttgccttgcgataaccattgtccgtccggaacaaccagtccatgtaaacaaatgtcgag 330
|||| |||||||||||| || || || | ||||||||||||||||||||||| || |||
Sbjct: 113 tttgtcttgcgataacccttatctgtgccaaacaaccagtccatgtaaacaaaagtagag 54
Query: 331 gcatagttccctgactttgtgtacagcactcggtgatggtagtcat 376
|| |||||||||||||| ||||| ||||| || |||||||||||||
Sbjct: 53 gcgtagttccctgacttggtgtagagcacacgatgatggtagtcat 8
>gb|CA019419.1|CA019419 HV11O17r HV Hordeum vulgare subsp. vulgare cDNA clone HV11O17
5-PRIME, mRNA sequence
Length = 692
Score = 65.9 bits (33), Expect = 1e-009
Identities = 62/72 (86%)
Strand = Plus / Minus
Query: 385 gagccaccgtacagtggaaggaaatttgatgggctccatggaaagtgatagccgctgtga 444
||||| || |||||||| ||||| |||||||||||||| || |||||||| || ||||||
Sbjct: 690 gagcctccatacagtggcaggaagtttgatgggctccaagggaagtgatatccactgtga 631
Query: 445 gcctcnaccgtc 456
|| || ||||||
Sbjct: 630 gcttccaccgtc 619
>gb|BU974803.1|BU974803 HB29C15r BC Hordeum vulgare subsp. vulgare cDNA clone HB29C15
5-PRIME, mRNA sequence
Length = 493
Score = 44.1 bits (22), Expect = 0.005
Identities = 39/45 (86%)
Strand = Plus / Minus
Query: 412 gatgggctccatggaaagtgatagccgctgtgagcctcnaccgtc 456
||||||||||| || |||||||| || |||||||| || ||||||
Sbjct: 493 gatgggctccaagggaagtgatatccactgtgagcttccaccgtc 449
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 31,625
Number of Sequences: 312970
Number of extensions: 31625
Number of successful extensions: 8235
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8215
Number of HSP's gapped (non-prelim): 16
length of query: 562
length of database: 175,134,539
effective HSP length: 19
effective length of query: 543
effective length of database: 169,188,109
effective search space: 91869143187
effective search space used: 91869143187
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)