BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594342.2.1
(715 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI960653.1|BI960653 HVSMEn0025K04f Hordeum vulgare rachi... 190 4e-047
gb|BI960123.1|BI960123 HVSMEn0023E24f Hordeum vulgare rachi... 188 2e-046
gb|BU994073.1|BU994073 HM05J23r HM Hordeum vulgare subsp. v... 157 6e-037
gb|CB880728.1|CB880728 HM07J23w HM Hordeum vulgare subsp. v... 155 2e-036
gb|BU984603.1|BU984603 HF04G18r HF Hordeum vulgare subsp. v... 125 2e-027
gb|BE421555.1|BE421555 HWM011cA.04r ITEC HWM Barley Leaf Li... 76 2e-012
gb|BF254439.3|BF254439 HVSMEf0004A10f Hordeum vulgare seedl... 76 2e-012
gb|BM444334.1|BM444334 EBem09_SQ004_N15_R embryo, 1 Day ger... 76 2e-012
gb|BG418646.2|BG418646 HVSMEk0023K20f Hordeum vulgare testa... 68 4e-010
gb|AL450671.1|AL450671 AL450671 Hordeum vulgare Barke etiol... 38 0.38
gb|BF255794.1|BF255794 HVSMEf0007N07f Hordeum vulgare seedl... 38 0.38
>gb|BI960653.1|BI960653 HVSMEn0025K04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K04f, mRNA sequence
Length = 652
Score = 190 bits (96), Expect = 4e-047
Identities = 176/203 (86%)
Strand = Plus / Plus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccaccttcctgttgccc 484
|||||||||||||| ||||||||||||||||||| ||| ||||||||||| | | ||||
Sbjct: 431 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccaccttgcggccgccc 490
Query: 485 agctcgtccacgacgctgaggtccttgcacacgtccaccttgaacgccaccacgcccgcc 544
||||||||||| ||||| |||||| |||||||||||||| |||||||||| ||| |||
Sbjct: 491 agctcgtccaccacgctcaggtccctgcacacgtccacccggaacgccaccgtgccggcc 550
Query: 545 tgcccgggctccagcgacaccttttcgaagcccagcaggggcttcgccggcgcgttgggc 604
| ||||||| ||||||||||||| ||||| || |||| | ||||||||||||||||| |
Sbjct: 551 tccccgggcgccagcgacaccttctcgaacccgagcaagtgcttcgccggcgcgttgtgg 610
Query: 605 accgccggcggcgacgagaacag 627
||| |||||||||| |||||||
Sbjct: 611 gccgacggcggcgacnagaacag 633
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 236 cccgtggatccaattctttc 255
>gb|BI960123.1|BI960123 HVSMEn0023E24f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023E24f, mRNA sequence
Length = 640
Score = 188 bits (95), Expect = 2e-046
Identities = 176/203 (86%)
Strand = Plus / Minus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccaccttcctgttgccc 484
|||||||||||||| ||||||||||||||||||| ||| |||||||| || | | ||||
Sbjct: 411 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccactttgcggccgccc 352
Query: 485 agctcgtccacgacgctgaggtccttgcacacgtccaccttgaacgccaccacgcccgcc 544
|||| |||||| |||||||||||| |||||||||||||| ||||||||| ||| |||
Sbjct: 351 agctggtccaccacgctgaggtccctgcacacgtccacccgcaacgccaccgtgccggcc 292
Query: 545 tgcccgggctccagcgacaccttttcgaagcccagcaggggcttcgccggcgcgttgggc 604
| ||||||| ||||||||||||| ||||| || |||||| ||||||||||||||||| |
Sbjct: 291 tccccgggcgccagcgacaccttctcgaacccgagcaggtgcttcgccggcgcgttgtgg 232
Query: 605 accgccggcggcgacgagaacag 627
||| ||||||||||||||||||
Sbjct: 231 gccgacggcggcgacgagaacag 209
>gb|BU994073.1|BU994073 HM05J23r HM Hordeum vulgare subsp. vulgare cDNA clone HM05J23
5-PRIME, mRNA sequence
Length = 543
Score = 157 bits (79), Expect = 6e-037
Identities = 139/159 (87%)
Strand = Plus / Plus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccaccttcctgttgccc 484
|||||||||||||| ||||||||||||||||||| ||| ||||||||||| | | ||||
Sbjct: 381 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccaccttgcggccgccc 440
Query: 485 agctcgtccacgacgctgaggtccttgcacacgtccaccttgaacgccaccacgcccgcc 544
||||||||||| ||||| |||||| |||||||||||||| |||||||||| ||| |||
Sbjct: 441 agctcgtccaccacgctcaggtccctgcacacgtccacccggaacgccaccgtgccggcc 500
Query: 545 tgcccgggctccagcgacaccttttcgaagcccagcagg 583
| ||||||| ||||||||||||| ||||| || ||||||
Sbjct: 501 tccccgggcgccagcgacaccttctcgaacccgagcagg 539
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 186 cccgtggatccaattctttc 205
>gb|CB880728.1|CB880728 HM07J23w HM Hordeum vulgare subsp. vulgare cDNA clone HM07J23
3-PRIME, mRNA sequence
Length = 553
Score = 155 bits (78), Expect = 2e-036
Identities = 138/158 (87%)
Strand = Plus / Minus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccaccttcctgttgccc 484
|||||||||||||| ||||||||||||||||||| ||| ||||||||||| | | ||||
Sbjct: 173 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccaccttgcggccgccc 114
Query: 485 agctcgtccacgacgctgaggtccttgcacacgtccaccttgaacgccaccacgcccgcc 544
||||||||||| ||||| |||||| |||||||||||||| |||||||||| ||| |||
Sbjct: 113 agctcgtccaccacgctcaggtccctgcacacgtccacccggaacgccaccgtgccggcc 54
Query: 545 tgcccgggctccagcgacaccttttcgaagcccagcag 582
| ||||||| ||||||||||||| ||||| || |||||
Sbjct: 53 tccccgggcgccagcgacaccttctcgaacccgagcag 16
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 367 cccgtggatccaattctttc 348
>gb|BU984603.1|BU984603 HF04G18r HF Hordeum vulgare subsp. vulgare cDNA clone HF04G18
5-PRIME, mRNA sequence
Length = 522
Score = 125 bits (63), Expect = 2e-027
Identities = 99/111 (89%)
Strand = Plus / Minus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccaccttcctgttgccc 484
|||||||||||||| ||||||||||||||||||| ||| ||||||||||| | | ||||
Sbjct: 122 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccaccttgcggccgccc 63
Query: 485 agctcgtccacgacgctgaggtccttgcacacgtccaccttgaacgccacc 535
||||||||||| ||||| |||||| |||||||||||||| ||||||||||
Sbjct: 62 agctcgtccaccacgctcaggtccctgcacacgtccacccggaacgccacc 12
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 317 cccgtggatccaattctttc 298
>gb|BE421555.1|BE421555 HWM011cA.04r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cA.04, mRNA sequence
Length = 1425
Score = 75.8 bits (38), Expect = 2e-012
Identities = 47/50 (94%)
Strand = Plus / Minus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccacctt 474
|||||||||||||| ||||||||||||||||||| ||| |||||||||||
Sbjct: 184 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccacctt 135
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 379 cccgtggatccaattctttc 360
>gb|BF254439.3|BF254439 HVSMEf0004A10f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0004A10f, mRNA sequence
Length = 824
Score = 75.8 bits (38), Expect = 2e-012
Identities = 47/50 (94%)
Strand = Plus / Minus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccacctt 474
|||||||||||||| ||||||||||||||||||| ||| |||||||||||
Sbjct: 60 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccacctt 11
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 255 cccgtggatccaattctttc 236
>gb|BM444334.1|BM444334 EBem09_SQ004_N15_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_N15 5', mRNA sequence
Length = 323
Score = 75.8 bits (38), Expect = 2e-012
Identities = 47/50 (94%)
Strand = Plus / Plus
Query: 425 gtgtgcttgaggtccccgacgtgcagcgtgtggctgcccagcgccacctt 474
|||||||||||||| ||||||||||||||||||| ||| |||||||||||
Sbjct: 274 gtgtgcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccacctt 323
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 250 cccgtggatccaattctttc 269
||||||||||||||||||||
Sbjct: 79 cccgtggatccaattctttc 98
>gb|BG418646.2|BG418646 HVSMEk0023K20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0023K20f, mRNA sequence
Length = 549
Score = 67.9 bits (34), Expect = 4e-010
Identities = 43/46 (93%)
Strand = Plus / Minus
Query: 429 gcttgaggtccccgacgtgcagcgtgtggctgcccagcgccacctt 474
|||||||||| ||||||||||||||||||| ||| |||||||||||
Sbjct: 543 gcttgaggtcgccgacgtgcagcgtgtggccgccgagcgccacctt 498
>gb|AL450671.1|AL450671 AL450671 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03L10u 5', mRNA sequence
Length = 610
Score = 38.2 bits (19), Expect = 0.38
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 349 cctccaaattccaactgcc 367
|||||||||||||||||||
Sbjct: 439 cctccaaattccaactgcc 457
>gb|BF255794.1|BF255794 HVSMEf0007N07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007N07f, mRNA sequence
Length = 1011
Score = 38.2 bits (19), Expect = 0.38
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 42 aataatcaatacttgcact 60
|||||||||||||||||||
Sbjct: 738 aataatcaatacttgcact 720
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 81,199
Number of Sequences: 312970
Number of extensions: 81199
Number of successful extensions: 22654
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 22635
Number of HSP's gapped (non-prelim): 19
length of query: 715
length of database: 175,134,539
effective HSP length: 19
effective length of query: 696
effective length of database: 169,188,109
effective search space: 117754923864
effective search space used: 117754923864
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)