BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591368.2.1
(543 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ468428.1|BQ468428 HM01D16T HM Hordeum vulgare subsp. v... 163 7e-039
gb|BM371273.2|BM371273 EBma08_SQ002_B15_R maternal, 28 DPA,... 40 0.073
gb|AL502553.1|AL502553 AL502553 Hordeum vulgare Barke roots... 38 0.29
gb|AL505273.1|AL505273 AL505273 Hordeum vulgare Barke roots... 38 0.29
gb|BJ460773.1|BJ460773 BJ460773 K. Sato unpublished cDNA li... 38 0.29
gb|BI776499.2|BI776499 EBpi05_SQ001_K01_R pistil, 8 DPA, no... 38 0.29
gb|BQ767849.1|BQ767849 EBro08_SQ010_C03_R root, 3 week, dro... 38 0.29
gb|BJ548493.1|BJ548493 BJ548493 K. Sato unpublished cDNA li... 38 0.29
gb|BJ548657.1|BJ548657 BJ548657 K. Sato unpublished cDNA li... 38 0.29
>gb|BQ468428.1|BQ468428 HM01D16T HM Hordeum vulgare subsp. vulgare cDNA clone HM01D16
5-PRIME, mRNA sequence
Length = 455
Score = 163 bits (82), Expect = 7e-039
Identities = 141/158 (89%), Gaps = 2/158 (1%)
Strand = Plus / Minus
Query: 385 ggccttggccttgctg-tgctggtgctggcgcacgaagcggcggatccggtcgagcgcga 443
|||||||||||| ||| ||||||||||||||||||||| | ||||||||||| ||||| |
Sbjct: 378 ggccttggcctt-ctgctgctggtgctggcgcacgaagagacggatccggtccagcgcca 320
Query: 444 cctccatggtgtcgtcgtccatgttggcgtagcagacgcggaaccagccgggctcgttgc 503
|| ||||||| ||||||||||||||||||| ||||||||||||||| |||||||| ||
Sbjct: 319 cccccatggtctcgtcgtccatgttggcgtggcagacgcggaaccacccgggctcccggc 260
Query: 504 agtggaacgacgtgccgggcgacacgttgagcttcacc 541
| |||||||||| || |||||||||||||||||||||
Sbjct: 259 accggaacgacgtccccggcgacacgttgagcttcacc 222
>gb|BM371273.2|BM371273 EBma08_SQ002_B15_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_B15 5', mRNA sequence
Length = 357
Score = 40.1 bits (20), Expect = 0.073
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 276 actgcggcgacagcaacgccaagg 299
|||||||| |||||||||||||||
Sbjct: 134 actgcggcaacagcaacgccaagg 157
>gb|AL502553.1|AL502553 AL502553 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07P07u 3', mRNA sequence
Length = 413
Score = 38.2 bits (19), Expect = 0.29
Identities = 64/79 (81%)
Strand = Plus / Plus
Query: 463 catgttggcgtagcagacgcggaaccagccgggctcgttgcagtggaacgacgtgccggg 522
|||||||||| |||| || | |||||| |||||||| ||| ||| |||||| || ||
Sbjct: 243 catgttggcgaagcacaccctgaaccatccgggctccgagcaatggcacgacgaccccgg 302
Query: 523 cgacacgttgagcttcacc 541
||| | |||||||||||||
Sbjct: 303 cgagatgttgagcttcacc 321
>gb|AL505273.1|AL505273 AL505273 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07P07V 5', mRNA sequence
Length = 656
Score = 38.2 bits (19), Expect = 0.29
Identities = 64/79 (81%)
Strand = Plus / Minus
Query: 463 catgttggcgtagcagacgcggaaccagccgggctcgttgcagtggaacgacgtgccggg 522
|||||||||| |||| || | |||||| |||||||| ||| ||| |||||| || ||
Sbjct: 454 catgttggcgaagcacaccctgaaccatccgggctccgagcaatggcacgacgaccccgg 395
Query: 523 cgacacgttgagcttcacc 541
||| | |||||||||||||
Sbjct: 394 cgagatgttgagcttcacc 376
>gb|BJ460773.1|BJ460773 BJ460773 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42c15 3', mRNA sequence
Length = 676
Score = 38.2 bits (19), Expect = 0.29
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 268 catcggggactgcggcgac 286
|||||||||||||||||||
Sbjct: 345 catcggggactgcggcgac 327
>gb|BI776499.2|BI776499 EBpi05_SQ001_K01_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ001_K01 5', mRNA sequence
Length = 560
Score = 38.2 bits (19), Expect = 0.29
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 268 catcggggactgcggcgac 286
|||||||||||||||||||
Sbjct: 487 catcggggactgcggcgac 505
>gb|BQ767849.1|BQ767849 EBro08_SQ010_C03_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_C03 5', mRNA sequence
Length = 660
Score = 38.2 bits (19), Expect = 0.29
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 363 gccgcgtggccgcccagcgctcg 385
||||||| |||||||||||||||
Sbjct: 42 gccgcgtcgccgcccagcgctcg 64
>gb|BJ548493.1|BJ548493 BJ548493 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal26e14 3', mRNA sequence
Length = 660
Score = 38.2 bits (19), Expect = 0.29
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 268 catcggggactgcggcgac 286
|||||||||||||||||||
Sbjct: 555 catcggggactgcggcgac 537
>gb|BJ548657.1|BJ548657 BJ548657 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal32e08 3', mRNA sequence
Length = 663
Score = 38.2 bits (19), Expect = 0.29
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 268 catcggggactgcggcgac 286
|||||||||||||||||||
Sbjct: 559 catcggggactgcggcgac 541
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 56,003
Number of Sequences: 312970
Number of extensions: 56003
Number of successful extensions: 17829
Number of sequences better than 0.5: 9
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17818
Number of HSP's gapped (non-prelim): 9
length of query: 543
length of database: 175,134,539
effective HSP length: 19
effective length of query: 524
effective length of database: 169,188,109
effective search space: 88654569116
effective search space used: 88654569116
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)