BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591131.2.1
(596 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU973592.1|BU973592 HB25G16r BC Hordeum vulgare subsp. v... 68 4e-010
gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA li... 48 3e-004
gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA li... 48 3e-004
gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. v... 48 3e-004
gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hyd... 48 3e-004
gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. v... 48 3e-004
gb|BG301240.1|BG301240 HVSMEb0020C04f Hordeum vulgare seedl... 46 0.001
gb|AL500971.1|AL500971 AL500971 Hordeum vulgare Barke roots... 42 0.020
gb|CA006626.1|CA006626 HU05D17r HU Hordeum vulgare subsp. v... 42 0.020
gb|CA023510.1|CA023510 HZ46H12r HZ Hordeum vulgare subsp. v... 42 0.020
gb|BF253497.3|BF253497 HVSMEf0001B16f Hordeum vulgare seedl... 40 0.081
>gb|BU973592.1|BU973592 HB25G16r BC Hordeum vulgare subsp. vulgare cDNA clone HB25G16
5-PRIME, mRNA sequence
Length = 261
Score = 67.9 bits (34), Expect = 4e-010
Identities = 91/110 (82%)
Strand = Plus / Minus
Query: 180 ctactcggcgaactgcttgccctcgaacgtctgcgcgaacatccagtccgccggcgccac 239
||||||| ||||||||||||| | || |||||||| ||||||||||||| ||||| ||
Sbjct: 120 ctactcgacgaactgcttgcctgcaaatgtctgcgcaaacatccagtccgaaggcgcgac 61
Query: 240 gctgtaggcggtgaccgtcctgcccctcccgccggtgacctcgaacgaca 289
| | || ||||| |||||| ||| | || |||||||||||||||||||
Sbjct: 60 gttatacatggtgatcgtcctcccctttcctccggtgacctcgaacgaca 11
Score = 38.2 bits (19), Expect = 0.32
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 180 ctactcggcgaactgcttgccct 202
||||||| |||||||||||||||
Sbjct: 166 ctactcgacgaactgcttgccct 144
>gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 5', mRNA sequence
Length = 654
Score = 48.1 bits (24), Expect = 3e-004
Identities = 33/36 (91%)
Strand = Plus / Minus
Query: 303 gaggtcggcgtcgcactgccagttctggccccagtt 338
|||||| |||| || |||||||||||||||||||||
Sbjct: 399 gaggtccgcgttgctctgccagttctggccccagtt 364
>gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 3', mRNA sequence
Length = 720
Score = 48.1 bits (24), Expect = 3e-004
Identities = 33/36 (91%)
Strand = Plus / Plus
Query: 303 gaggtcggcgtcgcactgccagttctggccccagtt 338
|||||| |||| || |||||||||||||||||||||
Sbjct: 368 gaggtccgcgttgctctgccagttctggccccagtt 403
>gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU04D11
5-PRIME, mRNA sequence
Length = 625
Score = 48.1 bits (24), Expect = 3e-004
Identities = 33/36 (91%)
Strand = Plus / Minus
Query: 303 gaggtcggcgtcgcactgccagttctggccccagtt 338
|||||| |||| || |||||||||||||||||||||
Sbjct: 337 gaggtccgcgttgctctgccagttctggccccagtt 302
>gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ005_H18 5', mRNA sequence
Length = 535
Score = 48.1 bits (24), Expect = 3e-004
Identities = 33/36 (91%)
Strand = Plus / Minus
Query: 303 gaggtcggcgtcgcactgccagttctggccccagtt 338
|||||| |||| || |||||||||||||||||||||
Sbjct: 201 gaggtccgcgttgctctgccagttctggccccagtt 166
>gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. vulgare cDNA clone HU12E04
3-PRIME, mRNA sequence
Length = 529
Score = 48.1 bits (24), Expect = 3e-004
Identities = 33/36 (91%)
Strand = Plus / Minus
Query: 303 gaggtcggcgtcgcactgccagttctggccccagtt 338
|||||| |||| || |||||||||||||||||||||
Sbjct: 323 gaggtccgcgttgctctgccagttctggccccagtt 288
>gb|BG301240.1|BG301240 HVSMEb0020C04f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0020C04f, mRNA sequence
Length = 906
Score = 46.1 bits (23), Expect = 0.001
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 317 actgccagttctggccccagttccgccccatcgggatccaccc 359
|||||||||||||||||||||| ||| ||| || ||||||||
Sbjct: 80 actgccagttctggccccagttgcgcgacatgggcatccaccc 38
>gb|AL500971.1|AL500971 AL500971 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02J12u 3', mRNA sequence
Length = 567
Score = 42.1 bits (21), Expect = 0.020
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 303 gaggtcggcgtcgcactgccagttctggcccca 335
|||||| |||| || ||||||||||||||||||
Sbjct: 465 gaggtccgcgttgctctgccagttctggcccca 497
>gb|CA006626.1|CA006626 HU05D17r HU Hordeum vulgare subsp. vulgare cDNA clone HU05D17
5-PRIME, mRNA sequence
Length = 534
Score = 42.1 bits (21), Expect = 0.020
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 318 ctgccagttctggccccagtt 338
|||||||||||||||||||||
Sbjct: 527 ctgccagttctggccccagtt 507
>gb|CA023510.1|CA023510 HZ46H12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ46H12
5-PRIME, mRNA sequence
Length = 429
Score = 42.1 bits (21), Expect = 0.020
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 318 ctgccagttctggccccagtt 338
|||||||||||||||||||||
Sbjct: 422 ctgccagttctggccccagtt 402
>gb|BF253497.3|BF253497 HVSMEf0001B16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001B16f, mRNA sequence
Length = 574
Score = 40.1 bits (20), Expect = 0.081
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 319 tgccagttctggccccagtt 338
||||||||||||||||||||
Sbjct: 444 tgccagttctggccccagtt 425
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 95,460
Number of Sequences: 312970
Number of extensions: 95460
Number of successful extensions: 28125
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 28087
Number of HSP's gapped (non-prelim): 37
length of query: 596
length of database: 175,134,539
effective HSP length: 19
effective length of query: 577
effective length of database: 169,188,109
effective search space: 97621538893
effective search space used: 97621538893
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)