BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591032.2.1
(735 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. v... 125 2e-027
gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subs... 90 1e-016
gb|CB875785.1|CB875785 HX09F12w HX Hordeum vulgare subsp. v... 90 1e-016
gb|BQ465179.1|BQ465179 HU02N24r HU Hordeum vulgare subsp. v... 76 2e-012
gb|BQ470446.1|BQ470446 HX03A05r HX Hordeum vulgare subsp. v... 76 2e-012
gb|BQ470603.1|BQ470603 HX03H14r HX Hordeum vulgare subsp. v... 76 2e-012
gb|CA007256.1|CA007256 HU07F09r HU Hordeum vulgare subsp. v... 76 2e-012
gb|BF622824.2|BF622824 HVSMEa0009C21f Hordeum vulgare seedl... 60 1e-007
gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. v... 54 7e-006
gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa... 48 4e-004
gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. v... 48 4e-004
gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. v... 48 4e-004
gb|AJ461221.1|AJ461221 AJ461221 S00002 Hordeum vulgare subs... 46 0.002
gb|AV834352.1|AV834352 AV834352 K. Sato unpublished cDNA li... 40 0.100
gb|AJ432746.1|AJ432746 AJ432746 S00002 Hordeum vulgare subs... 40 0.100
gb|BQ665321.1|BQ665321 HX03A05u HX Hordeum vulgare subsp. v... 40 0.100
gb|CA025871.1|CA025871 HZ53G05r HZ Hordeum vulgare subsp. v... 40 0.100
gb|AL510395.1|AL510395 AL510395 Hordeum vulgare Barke devel... 38 0.40
gb|BQ663490.1|BQ663490 HU02N24u HU Hordeum vulgare subsp. v... 38 0.40
>gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. vulgare cDNA clone HX06B10
3-PRIME, mRNA sequence
Length = 585
Score = 125 bits (63), Expect = 2e-027
Identities = 248/310 (80%)
Strand = Plus / Plus
Query: 323 gcctctcgatctcgacaggctttggtacctccggtgggcttgcgcaccttatgagcgccc 382
||||||| || || |||||||| || ||||| || ||||| || || | ||| | |||||
Sbjct: 266 gcctctcaatatcaacaggcttcggaacctctggagggctcgcacagcgtatcaacgccc 325
Query: 383 aattcacgccctcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggcca 442
||||||| |||||||| || |||||||| || ||||| ||||| |||||||| | |||
Sbjct: 326 aattcacaccctcaaaaaatggatgctgttttatctcagtggcaccacgcttacaaccca 385
Query: 443 gccgttgctggggctccttcacaagcagacccctgatcagatccctcgccgagaagctga 502
|||||| ||||||||||| ||||| || ||||| || | ||| || || |||||||| |
Sbjct: 386 accgttgttggggctccttaacaagtaggccccttattaaatctcttgctgagaagctca 445
Query: 503 cggccgggtactccgggaagcgcagctgctggccaatgacattgaacagcgtggcccggt 562
| | ||||| || ||||| |||| | |||| || |||||||||||||| || || ||||
Sbjct: 446 caactgggtattctgggaaacgcaacggctgaccgatgacattgaacagtgtcgctcggt 505
Query: 563 tgctggatcccttgaaaggcgtcttcccgaacagcagctcgtacaagaataccccgaagg 622
| | || || |||||||| || || |||||||| |||||||| ||||||| || ||||
Sbjct: 506 tacctgaacctttgaaaggtgtnttgccgaacagaagctcgtataagaatataccaaagg 565
Query: 623 tccaccagtc 632
||||||||||
Sbjct: 566 tccaccagtc 575
>gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200081A03F1, mRNA sequence
Length = 360
Score = 89.7 bits (45), Expect = 1e-016
Identities = 183/229 (79%)
Strand = Plus / Minus
Query: 323 gcctctcgatctcgacaggctttggtacctccggtgggcttgcgcaccttatgagcgccc 382
||||||| || || |||||||| || ||||| || ||||| || || | ||| | |||||
Sbjct: 235 gcctctcaatatcaacaggcttcggaacctctggagggctcgcacagcgtatcaacgccc 176
Query: 383 aattcacgccctcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggcca 442
||||||| |||||||| || |||||||| || ||||| ||||| |||||||| | |||
Sbjct: 175 aattcacaccctcaaaaaatggatgctgttttatctcagtggcaccacgcttacaaccca 116
Query: 443 gccgttgctggggctccttcacaagcagacccctgatcagatccctcgccgagaagctga 502
|||||| ||||||||||| ||||| || ||||| || | ||| || || |||||||| |
Sbjct: 115 accgttgttggggctccttaacaagtaggccccttattaaatctcttgctgagaagctca 56
Query: 503 cggccgggtactccgggaagcgcagctgctggccaatgacattgaacag 551
| | ||||| || ||||| |||| | |||| || ||||||||||||||
Sbjct: 55 caactgggtattctgggaaacgcaacggctgaccgatgacattgaacag 7
>gb|CB875785.1|CB875785 HX09F12w HX Hordeum vulgare subsp. vulgare cDNA clone HX09F12
3-PRIME, mRNA sequence
Length = 660
Score = 89.7 bits (45), Expect = 1e-016
Identities = 123/149 (82%)
Strand = Plus / Plus
Query: 352 tccggtgggcttgcgcaccttatgagcgcccaattcacgccctcaaagaagggatgctgc 411
||||| ||||| || || ||||| || ||||| ||||| |||||||||||||| ||||||
Sbjct: 461 tccggagggctcgcacatcttatcagggcccagttcacaccctcaaagaaggggtgctgc 520
Query: 412 ttgatctccgtggctccacgcttgtaggccagccgttgctggggctccttcacaagcaga 471
|| |||||||||||||| ||||||| | | ||||| || || || ||||| || |||||
Sbjct: 521 tttatctccgtggctccgcgcttgtggccgagccggtgttgcgggtccttgaccagcagt 580
Query: 472 cccctgatcagatccctcgccgagaagct 500
||||| |||| || || |||||||||||
Sbjct: 581 ccccttatcatgtctcttgccgagaagct 609
>gb|BQ465179.1|BQ465179 HU02N24r HU Hordeum vulgare subsp. vulgare cDNA clone HU02N24
5-PRIME, mRNA sequence
Length = 624
Score = 75.8 bits (38), Expect = 2e-012
Identities = 65/74 (87%)
Strand = Plus / Minus
Query: 622 gtccaccagtcgacggcgctgccatggccctcgcctttgatgatctcgggggccaggtac 681
|||||||| ||||||| |||||||||||| || ||| |||||||||| ||||| ||||||
Sbjct: 327 gtccaccaatcgacggagctgccatggccatctcctctgatgatctccggggcaaggtac 268
Query: 682 tcgtgcgtcccaac 695
|| || ||||||||
Sbjct: 267 tcatgggtcccaac 254
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 495 ggctccttcacaagcaaccctctgatcagatc 464
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 552 aagaagggatgccgcttgatctc 530
>gb|BQ470446.1|BQ470446 HX03A05r HX Hordeum vulgare subsp. vulgare cDNA clone HX03A05
5-PRIME, mRNA sequence
Length = 666
Score = 75.8 bits (38), Expect = 2e-012
Identities = 65/74 (87%)
Strand = Plus / Minus
Query: 622 gtccaccagtcgacggcgctgccatggccctcgcctttgatgatctcgggggccaggtac 681
|||||||| ||||||| |||||||||||| || ||| |||||||||| ||||| ||||||
Sbjct: 225 gtccaccaatcgacggagctgccatggccatctcctctgatgatctccggggcaaggtac 166
Query: 682 tcgtgcgtcccaac 695
|| || ||||||||
Sbjct: 165 tcatgggtcccaac 152
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 393 ggctccttcacaagcaaccctctgatcagatc 362
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 450 aagaagggatgccgcttgatctc 428
>gb|BQ470603.1|BQ470603 HX03H14r HX Hordeum vulgare subsp. vulgare cDNA clone HX03H14
5-PRIME, mRNA sequence
Length = 629
Score = 75.8 bits (38), Expect = 2e-012
Identities = 65/74 (87%)
Strand = Plus / Minus
Query: 622 gtccaccagtcgacggcgctgccatggccctcgcctttgatgatctcgggggccaggtac 681
|||||||| ||||||| |||||||||||| || ||| |||||||||| ||||| ||||||
Sbjct: 225 gtccaccaatcgacggagctgccatggccatctcctctgatgatctccggggcaaggtac 166
Query: 682 tcgtgcgtcccaac 695
|| || ||||||||
Sbjct: 165 tcatgggtcccaac 152
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 393 ggctccttcacaagcaaccctctgatcagatc 362
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 450 aagaagggatgccgcttgatctc 428
>gb|CA007256.1|CA007256 HU07F09r HU Hordeum vulgare subsp. vulgare cDNA clone HU07F09
5-PRIME, mRNA sequence
Length = 592
Score = 75.8 bits (38), Expect = 2e-012
Identities = 65/74 (87%)
Strand = Plus / Minus
Query: 622 gtccaccagtcgacggcgctgccatggccctcgcctttgatgatctcgggggccaggtac 681
|||||||| ||||||| |||||||||||| || ||| |||||||||| ||||| ||||||
Sbjct: 162 gtccaccaatcgacggagctgccatggccatctcctctgatgatctccggggcaaggtac 103
Query: 682 tcgtgcgtcccaac 695
|| || ||||||||
Sbjct: 102 tcatgggtcccaac 89
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 330 ggctccttcacaagcaaccctctgatcagatc 299
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 387 aagaagggatgccgcttgatctc 365
>gb|BF622824.2|BF622824 HVSMEa0009C21f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0009C21f, mRNA sequence
Length = 721
Score = 60.0 bits (30), Expect = 1e-007
Identities = 45/50 (90%)
Strand = Plus / Minus
Query: 373 atgagcgcccaattcacgccctcaaagaagggatgctgcttgatctccgt 422
||||| ||||| ||||||||||| ||||| || |||||||||||||||||
Sbjct: 214 atgagagcccagttcacgccctcgaagaacgggtgctgcttgatctccgt 165
>gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. vulgare cDNA clone HI01I09
3-PRIME, mRNA sequence
Length = 677
Score = 54.0 bits (27), Expect = 7e-006
Identities = 111/139 (79%)
Strand = Plus / Plus
Query: 530 gctggccaatgacattgaacagcgtggcccggttgctggatcccttgaaaggcgtcttcc 589
||||||||| |||||||| ||||| |||| ||| | || || ||||| || ||||| |
Sbjct: 507 gctggccaacaacattgaatagcgttgccctgttccccgaacctttgaatggagtcttgc 566
Query: 590 cgaacagcagctcgtacaagaataccccgaaggtccaccagtcgacggcgctgccatggc 649
|||| || || || |||||||||| || || ||||||||||| || || || ||||| |
Sbjct: 567 cgaaaagaagttcatacaagaatatgccaaaagtccaccagtctacagcactcccatgcc 626
Query: 650 cctcgcctttgatgatctc 668
||||||| || ||||||||
Sbjct: 627 cctcgccctttatgatctc 645
Score = 48.1 bits (24), Expect = 4e-004
Identities = 57/68 (83%)
Strand = Plus / Plus
Query: 394 tcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggccagccgttgctgg 453
|||||||| || |||||||| || || || ||||| ||||||||||||| || |||||
Sbjct: 371 tcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaatcgatgctgt 430
Query: 454 ggctcctt 461
||||||||
Sbjct: 431 ggctcctt 438
>gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0004P24f, mRNA sequence
Length = 632
Score = 48.1 bits (24), Expect = 4e-004
Identities = 57/68 (83%)
Strand = Plus / Minus
Query: 394 tcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggccagccgttgctgg 453
|||||||| || |||||||| || || || ||||| ||||||||||||| || |||||
Sbjct: 178 tcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaatcgatgctgt 119
Query: 454 ggctcctt 461
||||||||
Sbjct: 118 ggctcctt 111
>gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
5-PRIME, mRNA sequence
Length = 617
Score = 48.1 bits (24), Expect = 4e-004
Identities = 57/68 (83%)
Strand = Plus / Minus
Query: 394 tcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggccagccgttgctgg 453
|||||||| || |||||||| || || || ||||| ||||||||||||| || |||||
Sbjct: 286 tcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaatcgatgctgt 227
Query: 454 ggctcctt 461
||||||||
Sbjct: 226 ggctcctt 219
Score = 38.2 bits (19), Expect = 0.40
Identities = 82/103 (79%)
Strand = Plus / Minus
Query: 530 gctggccaatgacattgaacagcgtggcccggttgctggatcccttgaaaggcgtcttcc 589
||||||||| |||||||| ||||| |||| ||| | || || ||||| || ||||| |
Sbjct: 150 gctggccaacaacattgaatagcgttgccctgttccccgaacctttgaatggagtcttgc 91
Query: 590 cgaacagcagctcgtacaagaataccccgaaggtccaccagtc 632
|||| || || || |||||||||| || || |||||||||||
Sbjct: 90 cgaaaagaagttcatacaagaatatgccaaaagtccaccagtc 48
>gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
3-PRIME, mRNA sequence
Length = 589
Score = 48.1 bits (24), Expect = 4e-004
Identities = 57/68 (83%)
Strand = Plus / Plus
Query: 394 tcaaagaagggatgctgcttgatctccgtggctccacgcttgtaggccagccgttgctgg 453
|||||||| || |||||||| || || || ||||| ||||||||||||| || |||||
Sbjct: 398 tcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaatcgatgctgt 457
Query: 454 ggctcctt 461
||||||||
Sbjct: 458 ggctcctt 465
>gb|AJ461221.1|AJ461221 AJ461221 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200064D10F1, mRNA sequence
Length = 240
Score = 46.1 bits (23), Expect = 0.002
Identities = 41/47 (87%)
Strand = Plus / Minus
Query: 373 atgagcgcccaattcacgccctcaaagaagggatgctgcttgatctc 419
||||||||||| || || ||||| ||||| || ||||||||||||||
Sbjct: 67 atgagcgcccagttgacaccctcgaagaatgggtgctgcttgatctc 21
>gb|AV834352.1|AV834352 AV834352 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags15d19, mRNA sequence
Length = 619
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 458 ggctccttcacaagcaaccctctgatcagatc 489
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 401 aagaagggatgccgcttgatctc 423
>gb|AJ432746.1|AJ432746 AJ432746 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200023G10F1, mRNA sequence
Length = 353
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 84 ggctccttcacaagcaaccctctgatcagatc 53
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 141 aagaagggatgccgcttgatctc 119
>gb|BQ665321.1|BQ665321 HX03A05u HX Hordeum vulgare subsp. vulgare cDNA clone HX03A05
3-PRIME, mRNA sequence
Length = 594
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 486 ggctccttcacaagcaaccctctgatcagatc 517
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 429 aagaagggatgccgcttgatctc 451
>gb|CA025871.1|CA025871 HZ53G05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ53G05
5-PRIME, mRNA sequence
Length = 548
Score = 40.1 bits (20), Expect = 0.100
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 454 ggctccttcacaagcagacccctgatcagatc 485
|||||||||||||||| || |||||||||||
Sbjct: 72 ggctccttcacaagcaaccctctgatcagatc 41
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 129 aagaagggatgccgcttgatctc 107
>gb|AL510395.1|AL510395 AL510395 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04M17u 3',
mRNA sequence
Length = 640
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 427 aagaagggatgccgcttgatctc 449
>gb|BQ663490.1|BQ663490 HU02N24u HU Hordeum vulgare subsp. vulgare cDNA clone HU02N24
3-PRIME, mRNA sequence
Length = 488
Score = 38.2 bits (19), Expect = 0.40
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 397 aagaagggatgctgcttgatctc 419
|||||||||||| ||||||||||
Sbjct: 413 aagaagggatgccgcttgatctc 435
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 76,788
Number of Sequences: 312970
Number of extensions: 76788
Number of successful extensions: 22614
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 22579
Number of HSP's gapped (non-prelim): 35
length of query: 735
length of database: 175,134,539
effective HSP length: 19
effective length of query: 716
effective length of database: 169,188,109
effective search space: 121138686044
effective search space used: 121138686044
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)