BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521641.2.1
(594 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgar... 190 4e-047
gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. v... 188 1e-046
gb|BQ665678.1|BQ665678 HX04K17u HX Hordeum vulgare subsp. v... 174 2e-042
gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. v... 82 2e-014
gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 D... 52 2e-005
gb|BI956376.1|BI956376 HVSMEn0002P20f Hordeum vulgare rachi... 48 3e-004
gb|BM816708.1|BM816708 HB01G11_T3.ab1 HB Hordeum vulgare su... 44 0.005
gb|AJ461943.1|AJ461943 AJ461943 S00002 Hordeum vulgare subs... 40 0.080
gb|BQ664895.1|BQ664895 HX01E21w HX Hordeum vulgare subsp. v... 40 0.080
gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachi... 38 0.32
gb|BQ763901.1|BQ763901 EBro03_SQ005_P15_R root, 3 week, wat... 38 0.32
gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, wat... 38 0.32
>gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P133 5-PRIME, mRNA sequence
Length = 579
Score = 190 bits (96), Expect = 4e-047
Identities = 247/295 (83%), Gaps = 2/295 (0%)
Strand = Plus / Minus
Query: 301 ttagagctggccgtaggagtgcgcgaggtaggcgagcgcgccgacgagcggcgcgttgat 360
||||||||| ||| ||||||| ||||||||||||||||| |||||||| |||||||||||
Sbjct: 414 ttagagctgnccggaggagtgggcgaggtaggcgagcgccccgacgaggggcgcgttgat 355
Query: 361 gtaggtggccggctccgagtgctcgtggtcgttccgctcgtcggggaacctgtcctgcat 420
|||||||||||||||||||||||||| |||| | |||| |||||||||| ||| | |
Sbjct: 354 gtaggtggccggctccgagtgctcgtagtcgctgcgctggtcggggaactggtcgttctg 295
Query: 421 gtcgggtcccccgacgacggcaccgacgagcaggttgggattggccccacccgcgtacag 480
|| || || || |||||||| |||||| ||| ||| || |||||| | || | ||| ||
Sbjct: 294 gttcggcccgcccacgacggcgccgacgtgcacgttcgggttggccgcgccggagtagag 235
Query: 481 cgcggtgaacccctgcgagcaaccgatcctagccgggtgcgccgccacggacggcagcga 540
||||||||||||||||||| |||||| | ||||||||||||| ||||| ||||||||
Sbjct: 234 gccggtgaacccctgcgagcacccgatcttccccgggtgcgccgcgacggagggcagcga 175
Query: 541 cgaagcgcggtggtggatgcggc-gcgggtacttggcgccgtagcccaccatgta 594
|| || |||||||| | || | |||||||||||||||||| |||||||||||
Sbjct: 174 ggaggcccggtggtgca-gcttcttcgggtacttggcgccgtaccccaccatgta 121
>gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60B10
5-PRIME, mRNA sequence
Length = 408
Score = 188 bits (95), Expect = 1e-046
Identities = 215/255 (84%)
Strand = Plus / Minus
Query: 301 ttagagctggccgtaggagtgcgcgaggtaggcgagcgcgccgacgagcggcgcgttgat 360
||||||||||||| ||||||| ||||||||||||||||| |||||||| |||||||||||
Sbjct: 296 ttagagctggccggaggagtgggcgaggtaggcgagcgccccgacgaggggcgcgttgat 237
Query: 361 gtaggtggccggctccgagtgctcgtggtcgttccgctcgtcggggaacctgtcctgcat 420
|||||||||||||||||||||||||| |||| | |||| |||||||||| ||| | |
Sbjct: 236 gtaggtggccggctccgagtgctcgtagtcgctgcgctggtcggggaactggtcgttctg 177
Query: 421 gtcgggtcccccgacgacggcaccgacgagcaggttgggattggccccacccgcgtacag 480
|| || || || |||||||| |||||| ||| ||| || |||||| | || | ||| ||
Sbjct: 176 gttcggcccgcccacgacggcgccgacgtgcacgttcgggttggccgcgccggagtagag 117
Query: 481 cgcggtgaacccctgcgagcaaccgatcctagccgggtgcgccgccacggacggcagcga 540
||||||||||||||||||| |||||| | ||||||||||||| ||||| ||||||||
Sbjct: 116 gccggtgaacccctgcgagcacccgatcttccccgggtgcgccgcgacggagggcagcga 57
Query: 541 cgaagcgcggtggtg 555
|| || ||||||||
Sbjct: 56 ggaggcccggtggtg 42
>gb|BQ665678.1|BQ665678 HX04K17u HX Hordeum vulgare subsp. vulgare cDNA clone HX04K17
3-PRIME, mRNA sequence
Length = 517
Score = 174 bits (88), Expect = 2e-042
Identities = 199/236 (84%)
Strand = Plus / Plus
Query: 305 agctggccgtaggagtgcgcgaggtaggcgagcgcgccgacgagcggcgcgttgatgtag 364
||||||||| ||||||| ||||||||||||||||| |||||||| |||||||||||||||
Sbjct: 280 agctggccggaggagtgggcgaggtaggcgagcgccccgacgaggggcgcgttgatgtag 339
Query: 365 gtggccggctccgagtgctcgtggtcgttccgctcgtcggggaacctgtcctgcatgtcg 424
|||||||||||||||||||||| |||| | |||| |||||||||| ||| | | ||
Sbjct: 340 gtggccggctccgagtgctcgtagtcgctgcgctggtcggggaactggtcgttctggttc 399
Query: 425 ggtcccccgacgacggcaccgacgagcaggttgggattggccccacccgcgtacagcgcg 484
|| || || |||||||| |||||| ||| ||| || |||||| | || | ||| || ||
Sbjct: 400 ggcccgcccacgacggcgccgacgtgcacgttcgggttggccgcgccggagtagaggccg 459
Query: 485 gtgaacccctgcgagcaaccgatcctagccgggtgcgccgccacggacggcagcga 540
||||||||||||||||| |||||| | ||||||||||||| ||||| ||||||||
Sbjct: 460 gtgaacccctgcgagcacccgatcttccccgggtgcgccgcgacggagggcagcga 515
>gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47I13
5-PRIME, mRNA sequence
Length = 460
Score = 81.8 bits (41), Expect = 2e-014
Identities = 97/113 (85%), Gaps = 2/113 (1%)
Strand = Plus / Minus
Query: 483 cggtgaacccctgcgagcaaccgatcctagccgggtgcgccgccacggacggcagcgacg 542
||||||||||||||||||| |||||| | ||||||||||||| ||||| |||||||| |
Sbjct: 419 cggtgaacccctgcgagcacccgatcttccccgggtgcgccgcgacggagggcagcgagg 360
Query: 543 aagcgcggtggtggatgcggc-gcgggtacttggcgccgtagcccaccatgta 594
| || |||||||| | || | |||||||||||||||||| |||||||||||
Sbjct: 359 aggcccggtggtgca-gcttcttcgggtacttggcgccgtaccccaccatgta 308
>gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ002_M04 5', mRNA sequence
Length = 464
Score = 52.0 bits (26), Expect = 2e-005
Identities = 29/30 (96%)
Strand = Plus / Minus
Query: 565 cgggtacttggcgccgtagcccaccatgta 594
|||||||||||||||||| |||||||||||
Sbjct: 443 cgggtacttggcgccgtaccccaccatgta 414
>gb|BI956376.1|BI956376 HVSMEn0002P20f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0002P20f, mRNA sequence
Length = 829
Score = 48.1 bits (24), Expect = 3e-004
Identities = 75/92 (81%)
Strand = Plus / Minus
Query: 503 ccgatcctagccgggtgcgccgccacggacggcagcgacgaagcgcggtggtggatgcgg 562
|||||||| ||||||||||||||||| ||||| | || ||||||||||||| |||
Sbjct: 107 ccgatcctcgccgggtgcgccgccaccgacgggatggagctgccgcggtggtggatccgg 48
Query: 563 cgcgggtacttggcgccgtagcccaccatgta 594
|||||| || | ||||||||| ||||||||
Sbjct: 47 cgcgggaaccgcgggccgtagccgaccatgta 16
>gb|BM816708.1|BM816708 HB01G11_T3.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB01G11_T3.ab1 similar to (AC006551) Similar to LeOPT1
[Lycopersicon esculentum] [Arabidopsis
thaliana],(AC006551) Similar to peptide transporter
[Arabidopsis thaliana],(AC069273) oligopeptide
transporter, putative [Arabidopsis thaliana],, mRNA
sequence
Length = 861
Score = 44.1 bits (22), Expect = 0.005
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 331 ggcgagcgcgccgacgagcggc 352
||||||||||||||||||||||
Sbjct: 298 ggcgagcgcgccgacgagcggc 277
>gb|AJ461943.1|AJ461943 AJ461943 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058H03F1, mRNA sequence
Length = 300
Score = 40.1 bits (20), Expect = 0.080
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 437 acggcaccgacgagcaggttgggattgg 464
||||| ||||||||||||||||| ||||
Sbjct: 48 acggcgccgacgagcaggttggggttgg 21
>gb|BQ664895.1|BQ664895 HX01E21w HX Hordeum vulgare subsp. vulgare cDNA clone HX01E21
3-PRIME, mRNA sequence
Length = 513
Score = 40.1 bits (20), Expect = 0.080
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 437 acggcaccgacgagcaggttgggattgg 464
||||| ||||||||||||||||| ||||
Sbjct: 428 acggcgccgacgagcaggttggggttgg 455
>gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K02f, mRNA sequence
Length = 632
Score = 38.2 bits (19), Expect = 0.32
Identities = 49/59 (83%)
Strand = Plus / Minus
Query: 406 gaacctgtcctgcatgtcgggtcccccgacgacggcaccgacgagcaggttgggattgg 464
||||||||||| | |||||| || ||||||| ||| || ||||||| |||||| ||||
Sbjct: 476 gaacctgtcctcccggtcgggcccgccgacgatggcgcccacgagcacgttggggttgg 418
Score = 38.2 bits (19), Expect = 0.32
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 566 gggtacttggcgccgtagcccac 588
||||||||||||||||| |||||
Sbjct: 313 gggtacttggcgccgtaccccac 291
>gb|BQ763901.1|BQ763901 EBro03_SQ005_P15_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_P15 5', mRNA sequence
Length = 643
Score = 38.2 bits (19), Expect = 0.32
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 397 ctcgtcggggaacctgtcc 415
|||||||||||||||||||
Sbjct: 490 ctcgtcggggaacctgtcc 472
>gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_D24 5', mRNA sequence
Length = 613
Score = 38.2 bits (19), Expect = 0.32
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 566 gggtacttggcgccgtagcccac 588
||||||||||||||||| |||||
Sbjct: 387 gggtacttggcgccgtaccccac 365
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 62,418
Number of Sequences: 312970
Number of extensions: 62418
Number of successful extensions: 16979
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16944
Number of HSP's gapped (non-prelim): 35
length of query: 594
length of database: 175,134,539
effective HSP length: 19
effective length of query: 575
effective length of database: 169,188,109
effective search space: 97283162675
effective search space used: 97283162675
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)