BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448753.2.1
(537 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE215918.2|BE215918 HV_CEb0008N12f Hordeum vulgare seedl... 78 3e-013
gb|BF617538.3|BF617538 HVSMEc0017O04f Hordeum vulgare seedl... 78 3e-013
gb|BQ459489.1|BQ459489 HA08P01r HA Hordeum vulgare subsp. v... 78 3e-013
gb|BU995403.1|BU995403 HM10C12r HM Hordeum vulgare subsp. v... 78 3e-013
gb|CA020711.1|CA020711 HZ37E17r HZ Hordeum vulgare subsp. v... 78 3e-013
gb|BJ464811.1|BJ464811 BJ464811 K. Sato unpublished cDNA li... 70 8e-011
gb|BQ470106.1|BQ470106 HX01M17T HX Hordeum vulgare subsp. v... 40 0.072
>gb|BE215918.2|BE215918 HV_CEb0008N12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0008N12f, mRNA sequence
Length = 517
Score = 77.8 bits (39), Expect = 3e-013
Identities = 135/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 201 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 260
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| ||||||||||||
Sbjct: 261 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatc 320
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 321 gtagaccgcgacactggaaggtctagaggttttggctttataactta 367
Score = 50.1 bits (25), Expect = 8e-005
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 53 gctaataagcttggtggtttgctcaagaa 81
|||||||||||||||||| ||||||||||
Sbjct: 141 gctaataagcttggtggtctgctcaagaa 169
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 382 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 441
Query: 363 tta 365
|||
Sbjct: 442 tta 444
>gb|BF617538.3|BF617538 HVSMEc0017O04f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0017O04f, mRNA sequence
Length = 762
Score = 77.8 bits (39), Expect = 3e-013
Identities = 135/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 191 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 250
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| ||||||||||||
Sbjct: 251 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatc 310
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 311 gtagaccgcgacactggaaggtctagaggttttggctttataactta 357
Score = 50.1 bits (25), Expect = 8e-005
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 53 gctaataagcttggtggtttgctcaagaa 81
|||||||||||||||||| ||||||||||
Sbjct: 131 gctaataagcttggtggtctgctcaagaa 159
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 372 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 431
Query: 363 tta 365
|||
Sbjct: 432 tta 434
>gb|BQ459489.1|BQ459489 HA08P01r HA Hordeum vulgare subsp. vulgare cDNA clone HA08P01
5-PRIME, mRNA sequence
Length = 513
Score = 77.8 bits (39), Expect = 3e-013
Identities = 135/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 212 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 271
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| ||||||||||||
Sbjct: 272 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatc 331
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 332 gtagaccgcgacactggaaggtctagaggttttggctttataactta 378
Score = 50.1 bits (25), Expect = 8e-005
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 53 gctaataagcttggtggtttgctcaagaa 81
|||||||||||||||||| ||||||||||
Sbjct: 152 gctaataagcttggtggtctgctcaagaa 180
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 393 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 452
Query: 363 tta 365
|||
Sbjct: 453 tta 455
>gb|BU995403.1|BU995403 HM10C12r HM Hordeum vulgare subsp. vulgare cDNA clone HM10C12
5-PRIME, mRNA sequence
Length = 478
Score = 77.8 bits (39), Expect = 3e-013
Identities = 135/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 63 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 122
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| ||||||||||||
Sbjct: 123 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatc 182
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 183 gtagaccgcgacactggaaggtctagaggttttggctttataactta 229
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 244 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 303
Query: 363 tta 365
|||
Sbjct: 304 tta 306
>gb|CA020711.1|CA020711 HZ37E17r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ37E17
5-PRIME, mRNA sequence
Length = 587
Score = 77.8 bits (39), Expect = 3e-013
Identities = 135/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 220 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 279
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| ||||||||||||
Sbjct: 280 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatc 339
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 340 gtagaccgcgacactggaaggtctagaggttttggctttataactta 386
Score = 50.1 bits (25), Expect = 8e-005
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 53 gctaataagcttggtggtttgctcaagaa 81
|||||||||||||||||| ||||||||||
Sbjct: 160 gctaataagcttggtggtctgctcaagaa 188
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 401 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 460
Query: 363 tta 365
|||
Sbjct: 461 tta 463
>gb|BJ464811.1|BJ464811 BJ464811 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36c02 5', mRNA sequence
Length = 587
Score = 69.9 bits (35), Expect = 8e-011
Identities = 134/167 (80%)
Strand = Plus / Plus
Query: 122 gcaatcagatgcatgtcatcatccaagctttttgttggagggctttcttatggaaccgat 181
||||| |||||||||||||| || ||||| || |||||||||||||| ||| || ||
Sbjct: 224 gcaataagatgcatgtcatcctcgaagctcttcgttggagggctttcgtataccacagac 283
Query: 182 gatcacagtctcagagacgagtttgccaaatatggacaagttattgaagctaagattatc 241
|| |||||| ||||| | ||| || | |||||| || ||||||| |||||||||||
Sbjct: 284 gagggcagtctgagagatgccttttcccattatggagaaattattgatgctaagattatt 343
Query: 242 cttgatcgtgagtctgggaggtccagaggttttggctttataactta 288
| || || || |||| ||||| |||||||||||||||||||||||
Sbjct: 344 gtagaccgcgacactggaaggtctagaggttttggctttataactta 390
Score = 50.1 bits (25), Expect = 8e-005
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 53 gctaataagcttggtggtttgctcaagaa 81
|||||||||||||||||| ||||||||||
Sbjct: 164 gctaataagcttggtggtctgctcaagaa 192
Score = 38.2 bits (19), Expect = 0.29
Identities = 52/63 (82%)
Strand = Plus / Plus
Query: 303 aggcttcggctgccatcactgccatggatggaaagactctggatggacggaacatacggg 362
||||||| ||||||||| ||| ||||||||||| ||| ||||||| ||| ||||||
Sbjct: 405 aggcttcatctgccatcatggccttggatggaaaggatctacatggacgcaacctacggg 464
Query: 363 tta 365
|||
Sbjct: 465 tta 467
>gb|BQ470106.1|BQ470106 HX01M17T HX Hordeum vulgare subsp. vulgare cDNA clone HX01M17
5-PRIME, mRNA sequence
Length = 533
Score = 40.1 bits (20), Expect = 0.072
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 143 tccaagctttttgttggagg 162
||||||||||||||||||||
Sbjct: 16 tccaagctttttgttggagg 35
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 34,787
Number of Sequences: 312970
Number of extensions: 34787
Number of successful extensions: 9557
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9530
Number of HSP's gapped (non-prelim): 26
length of query: 537
length of database: 175,134,539
effective HSP length: 19
effective length of query: 518
effective length of database: 169,188,109
effective search space: 87639440462
effective search space used: 87639440462
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)