BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441276.2.1
(1796 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG299933.1|BG299933 HVSMEa0022J07f Hordeum vulgare seedl... 141 9e-032
gb|BF622955.2|BF622955 HVSMEa0012E13f Hordeum vulgare seedl... 105 5e-021
gb|BG343130.1|BG343130 HVSMEg0002J04f Hordeum vulgare pre-a... 50 3e-004
gb|AJ461846.1|AJ461846 AJ461846 S00002 Hordeum vulgare subs... 48 0.001
gb|AJ461847.1|AJ461847 AJ461847 S00002 Hordeum vulgare subs... 48 0.001
gb|BU969636.1|BU969636 HB12B24r BC Hordeum vulgare subsp. v... 48 0.001
gb|CA031681.1|CA031681 HX10N04r HX Hordeum vulgare subsp. v... 48 0.001
>gb|BG299933.1|BG299933 HVSMEa0022J07f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0022J07f, mRNA sequence
Length = 787
Score = 141 bits (71), Expect = 9e-032
Identities = 137/159 (86%)
Strand = Plus / Minus
Query: 1090 tgttactcgcatcgttggagcaacttcgttcatcaatgtactgcatgttcgttgcttcgc 1149
|||||||| ||| || ||||||| ||||||||| |||||||||| ||| ||||||| |
Sbjct: 159 tgttactcccattattagagcaacctcgttcatcgatgtactgcaagtttcttgcttcac 100
Query: 1150 atggctgagaagttgcaacagcagcattgtcatgagttctataacccagtaattcttgac 1209
||||||| ||||| |||||||||| | ||||||||||||||||||| |||||||||||
Sbjct: 99 atggctgtgaagtagcaacagcagtaccatcatgagttctataacccaataattcttgac 40
Query: 1210 cttgcaagaccttgtggaattgggcagattccgcaaaat 1248
|||||||||||||| ||| ||| ||||| || |||||||
Sbjct: 39 cttgcaagaccttgcggagttgtgcagactctgcaaaat 1
Score = 105 bits (53), Expect = 5e-021
Identities = 86/97 (88%)
Strand = Plus / Minus
Query: 936 gcatcgaccagagttcctctgtagggatgaaatacttcttgaccttgcaagaccttttgg 995
||||| |||||| |||||| ||| ||| | || |||||||||||||||||||||||||||
Sbjct: 307 gcatcaaccagacttcctcggtacggacggaaaacttcttgaccttgcaagaccttttgg 248
Query: 996 aatcttggagactccccaaagcctgagcaacggtagg 1032
||||| |||||||||||||||| |||||| ||||||
Sbjct: 247 aatctctgagactccccaaagccagagcaatggtagg 211
Score = 48.1 bits (24), Expect = 0.001
Identities = 27/28 (96%)
Strand = Plus / Minus
Query: 1202 ttcttgaccttgcaagaccttgtggaat 1229
||||||||||||||||||||| ||||||
Sbjct: 272 ttcttgaccttgcaagaccttttggaat 245
Score = 44.1 bits (22), Expect = 0.016
Identities = 25/26 (96%)
Strand = Plus / Minus
Query: 966 aatacttcttgaccttgcaagacctt 991
|||| |||||||||||||||||||||
Sbjct: 52 aataattcttgaccttgcaagacctt 27
>gb|BF622955.2|BF622955 HVSMEa0012E13f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0012E13f, mRNA sequence
Length = 850
Score = 105 bits (53), Expect = 5e-021
Identities = 86/97 (88%)
Strand = Plus / Minus
Query: 936 gcatcgaccagagttcctctgtagggatgaaatacttcttgaccttgcaagaccttttgg 995
||||| |||||| |||||| ||| ||| | || |||||||||||||||||||||||||||
Sbjct: 135 gcatcaaccagacttcctcggtacggacggaaaacttcttgaccttgcaagaccttttgg 76
Query: 996 aatcttggagactccccaaagcctgagcaacggtagg 1032
||||| |||||||||||||||| |||||| ||||||
Sbjct: 75 aatctctgagactccccaaagccagagcaatggtagg 39
Score = 48.1 bits (24), Expect = 0.001
Identities = 27/28 (96%)
Strand = Plus / Minus
Query: 1202 ttcttgaccttgcaagaccttgtggaat 1229
||||||||||||||||||||| ||||||
Sbjct: 100 ttcttgaccttgcaagaccttttggaat 73
Score = 40.1 bits (20), Expect = 0.25
Identities = 49/56 (87%), Gaps = 2/56 (3%)
Strand = Plus / Minus
Query: 360 acgtcaatcaccctagcaacaacaggcctct-gctcatgaacggtggcacagctgt 414
||||||||| |||| |||||||||| |||| |||||||||| |||||||| ||||
Sbjct: 671 acgtcaatcgccctgccaacaacagg-ctctggctcatgaacagtggcacaactgt 617
>gb|BG343130.1|BG343130 HVSMEg0002J04f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002J04f, mRNA sequence
Length = 856
Score = 50.1 bits (25), Expect = 3e-004
Identities = 28/29 (96%)
Strand = Plus / Minus
Query: 1200 aattcttgaccttgcaagaccttgtggaa 1228
||||||||||||||||||||| |||||||
Sbjct: 254 aattcttgaccttgcaagaccctgtggaa 226
Score = 40.1 bits (20), Expect = 0.25
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 971 ttcttgaccttgcaagacct 990
||||||||||||||||||||
Sbjct: 447 ttcttgaccttgcaagacct 428
Score = 40.1 bits (20), Expect = 0.25
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1202 ttcttgaccttgcaagacct 1221
||||||||||||||||||||
Sbjct: 447 ttcttgaccttgcaagacct 428
>gb|AJ461846.1|AJ461846 AJ461846 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200033G05F1, mRNA sequence
Length = 240
Score = 48.1 bits (24), Expect = 0.001
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 970 cttcttgaccttgcaagacctttt 993
||||||||||||||||||||||||
Sbjct: 27 cttcttgaccttgcaagacctttt 50
Score = 42.1 bits (21), Expect = 0.063
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1202 ttcttgaccttgcaagacctt 1222
|||||||||||||||||||||
Sbjct: 28 ttcttgaccttgcaagacctt 48
>gb|AJ461847.1|AJ461847 AJ461847 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200033G05F2, mRNA sequence
Length = 300
Score = 48.1 bits (24), Expect = 0.001
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 970 cttcttgaccttgcaagacctttt 993
||||||||||||||||||||||||
Sbjct: 27 cttcttgaccttgcaagacctttt 50
Score = 42.1 bits (21), Expect = 0.063
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1202 ttcttgaccttgcaagacctt 1222
|||||||||||||||||||||
Sbjct: 28 ttcttgaccttgcaagacctt 48
>gb|BU969636.1|BU969636 HB12B24r BC Hordeum vulgare subsp. vulgare cDNA clone HB12B24
5-PRIME, mRNA sequence
Length = 449
Score = 48.1 bits (24), Expect = 0.001
Identities = 36/40 (90%)
Strand = Plus / Minus
Query: 1181 atgagttctataacccagtaattcttgaccttgcaagacc 1220
|||||||| | |||||| |||||||||||||||||||||
Sbjct: 259 atgagttccagaacccatcaattcttgaccttgcaagacc 220
>gb|CA031681.1|CA031681 HX10N04r HX Hordeum vulgare subsp. vulgare cDNA clone HX10N04
5-PRIME, mRNA sequence
Length = 518
Score = 48.1 bits (24), Expect = 0.001
Identities = 36/40 (90%)
Strand = Plus / Minus
Query: 1181 atgagttctataacccagtaattcttgaccttgcaagacc 1220
|||||||| | |||||| |||||||||||||||||||||
Sbjct: 505 atgagttccagaacccatcaattcttgaccttgcaagacc 466
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 155,723
Number of Sequences: 312970
Number of extensions: 155723
Number of successful extensions: 40787
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 40755
Number of HSP's gapped (non-prelim): 32
length of query: 1796
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1777
effective length of database: 169,188,109
effective search space: 300647269693
effective search space used: 300647269693
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)