BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441058.2.1
(1000 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ462645.1|BJ462645 BJ462645 K. Sato unpublished cDNA li... 529 e-149
gb|BQ663683.1|BQ663683 HU03J08u HU Hordeum vulgare subsp. v... 422 e-117
gb|AV918442.1|AV918442 AV918442 K. Sato unpublished cDNA li... 420 e-116
gb|BQ465414.1|BQ465414 HU03J08r HU Hordeum vulgare subsp. v... 236 1e-060
gb|AV837011.1|AV837011 AV837011 K. Sato unpublished cDNA li... 86 3e-015
gb|BJ467748.1|BJ467748 BJ467748 K. Sato unpublished cDNA li... 64 9e-009
gb|CA027638.1|CA027638 HZ59I23r HZ Hordeum vulgare subsp. v... 40 0.14
>gb|BJ462645.1|BJ462645 BJ462645 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29k09 5', mRNA sequence
Length = 626
Score = 529 bits (267), Expect = e-149
Identities = 530/616 (86%), Gaps = 4/616 (0%)
Strand = Plus / Plus
Query: 1 gaactgctgcactatacggaaaagccggagacttt-gtgagtgatctcataaattgcgga 59
|||||| | |||||||| ||||| || |||||||| ||||||||||||||||||||||||
Sbjct: 10 gaactgttacactatacagaaaaaccagagacttttgtgagtgatctcataaattgcgga 69
Query: 60 ga-tatatatttactcccgatatccttagcgccattgaggatgtcttaaaacagaaaaaa 118
| ||||||||||||||| |||| |||| || || ||||||||||| |||||||| |||
Sbjct: 70 gtctatatatttactcccaatatacttaatgcaatcgaggatgtcttgaaacagaagaaa 129
Query: 119 gacagagcaaatttgnnnnnnnngtatctagcattgaagctcttcattcagcaactaagg 178
|| ||||| |||||| ||||| ||| ||||||||||||| || |||| ||||
Sbjct: 130 gatagagctaatttgcgccgt--gtatccagctttgaagctcttcagtccgcaatgaagg 187
Query: 179 cgcttccaccagactttgttaggttagatcaagacattttatcccctcgagcgggaaaga 238
| ||||| |||| | |||||||||||||||||| || ||||| |||| ||| |||||||
Sbjct: 188 caattccagcagattatgttaggttagatcaagatatcttatctcctctagcaggaaaga 247
Query: 239 aggagctgtacacataccagacacttgatttctgggaacagatcaaaaccccagggatgt 298
|||| || ||||||||| ||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 248 aggaactttacacatacgagacacttgatttttgggaacagatcaagactccagggatgt 307
Query: 299 ctttgagatgctctgggttatatctctctcagtttcgccatacctctcctcatcttttag 358
|||||||||||||||| |||||||||| |||||||||||||||||||||||||| || |
Sbjct: 308 ctttgagatgctctggtctatatctctcccagtttcgccatacctctcctcatctcttgg 367
Query: 359 cctcaggagatggcaaaagaactgccactattgttggcgatgtctatatccatccatctg 418
|||| ||||||||||||| |||||||||| | || || || || |||||||||||||
Sbjct: 368 cctctggagatggcaaaaagggtgccactattataggtgacgtgtacatccatccatctg 427
Query: 419 ccaaggtgcatcctacttcaaagattggcccaaatgtctctatatcagcaaatgcacgcg 478
| ||||| || | ||| ||||||||||||| |||||||| ||||||||||||||||||
Sbjct: 428 caaaggttcacctaactgcaaagattggccccaatgtctcaatatcagcaaatgcacgca 487
Query: 479 ttggagctggtgccaggcttatcaattgcataattctggatgatgttgaaattatggaaa 538
||||||| || |||||||||||||||||||| |||||||||||||||||||||||||| |
Sbjct: 488 ttggagcaggagccaggcttatcaattgcatcattctggatgatgttgaaattatggaga 547
Query: 539 atgctgttgttatacattcaattgtagggtggaaatcatccattggaaaatggtcacgtg 598
|||| ||||||||||||||||| ||||||||||| ||| | | || |||||||||||||
Sbjct: 548 atgcagttgttatacattcaatagtagggtggaagtcaacagtagggaaatggtcacgtg 607
Query: 599 tacagggcgaaggtga 614
||||||| ||||||||
Sbjct: 608 tacagggtgaaggtga 623
>gb|BQ663683.1|BQ663683 HU03J08u HU Hordeum vulgare subsp. vulgare cDNA clone HU03J08
3-PRIME, mRNA sequence
Length = 619
Score = 422 bits (213), Expect = e-117
Identities = 315/349 (90%)
Strand = Plus / Minus
Query: 406 atccatccatctgccaaggtgcatcctacttcaaagattggcccaaatgtctctatatca 465
|||||||||||||| ||||| || | ||| ||||||||||||| |||||||| ||||||
Sbjct: 619 atccatccatctgcaaaggttcacctaactgcaaagattggccccaatgtctcaatatca 560
Query: 466 gcaaatgcacgcgttggagctggtgccaggcttatcaattgcataattctggatgatgtt 525
|||||||||||| ||||||| || |||||||||||||||||||| |||||||||||||||
Sbjct: 559 gcaaatgcacgcattggagcaggagccaggcttatcaattgcatcattctggatgatgtt 500
Query: 526 gaaattatggaaaatgctgttgttatacattcaattgtagggtggaaatcatccattgga 585
||||||||||| ||||| ||||||||||||||||| ||||||||||| ||| | | ||
Sbjct: 499 gaaattatggagaatgcagttgttatacattcaatagtagggtggaagtcaacagtaggg 440
Query: 586 aaatggtcacgtgtacagggcgaaggtgatcacaatgccaaacttggcattactattctt 645
|||||||||||||||||||| |||||||| ||||||||||||||||| || || ||||||
Sbjct: 439 aaatggtcacgtgtacagggtgaaggtgaccacaatgccaaacttggtatcaccattctt 380
Query: 646 ggtgaagccgttgatgttgaagatgaagtagttgtagttaacagcatcgtgctcccaaat 705
|||||||||||||||||||||||||| ||||||||| ||||||||||||||||||||||
Sbjct: 379 ggtgaagccgttgatgttgaagatgaggtagttgtaactaacagcatcgtgctcccaaat 320
Query: 706 aaaactctcaatgtcagtgtccaagaggagatcatcctataataaccat 754
||||||||||||| ||| || ||||| ||||||||||||||||| ||||
Sbjct: 319 aaaactctcaatgccagcgtgcaagatgagatcatcctataatatccat 271
>gb|AV918442.1|AV918442 AV918442 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21b04 3', mRNA sequence
Length = 587
Score = 420 bits (212), Expect = e-116
Identities = 333/374 (89%)
Strand = Plus / Minus
Query: 381 tgccactattgttggcgatgtctatatccatccatctgccaaggtgcatcctacttcaaa 440
|||||||||| | || || || || |||||||||| ||| ||||| || | ||| ||||
Sbjct: 582 tgccactattataggtgangtgtacatccatccatntgcaaaggttcacctaactgcaaa 523
Query: 441 gattggcccaaatgtctctatatcagcaaatgcacgcgttggagctggtgccaggcttat 500
||||||||| |||||||| |||||||||||||||||| ||||||| || |||||||||||
Sbjct: 522 gattggccccaatgtctcaatatcagcaaatgcacgcattggagcaggagccaggcttat 463
Query: 501 caattgcataattctggatgatgttgaaattatggaaaatgctgttgttatacattcaat 560
||||||||| |||||||||||||||||||||||||| ||||| |||||||||||||||||
Sbjct: 462 caattgcatcattctggatgatgttgaaattatggagaatgcagttgttatacattcaat 403
Query: 561 tgtagggtggaaatcatccattggaaaatggtcacgtgtacagggcgaaggtgatcacaa 620
||||||||||| ||| | | || |||||||||||||||||||| |||||||| |||||
Sbjct: 402 agtagggtggaagtcaacagtagggaaatggtcacgtgtacagggtgaaggtgaccacaa 343
Query: 621 tgccaaacttggcattactattcttggtgaagccgttgatgttgaagatgaagtagttgt 680
|||||||||||| || || |||||||||||||||||||||||||||||||| ||||||||
Sbjct: 342 tgccaaacttggtatcaccattcttggtgaagccgttgatgttgaagatgaggtagttgt 283
Query: 681 agttaacagcatcgtgctcccaaataaaactctcaatgtcagtgtccaagaggagatcat 740
| ||||||||||||||||||||||||||||||||||| ||| || ||||| ||||||||
Sbjct: 282 aactaacagcatcgtgctcccaaataaaactctcaatgccagcgtgcaagatgagatcat 223
Query: 741 cctataataaccat 754
||||||||| ||||
Sbjct: 222 cctataatatccat 209
>gb|BQ465414.1|BQ465414 HU03J08r HU Hordeum vulgare subsp. vulgare cDNA clone HU03J08
5-PRIME, mRNA sequence
Length = 685
Score = 236 bits (119), Expect = 1e-060
Identities = 268/316 (84%), Gaps = 4/316 (1%)
Strand = Plus / Plus
Query: 1 gaactgctgcactatacggaaaagccggagacttt-gtgagtgatctcataaattgcgga 59
|||||| | |||||||| ||||| || |||||||| ||||||||||||||||||||||||
Sbjct: 367 gaactgttacactatacagaaaaaccagagacttttgtgagtgatctcataaattgcgga 426
Query: 60 ga-tatatatttactcccgatatccttagcgccattgaggatgtcttaaaacagaaaaaa 118
| ||||||||||||||| |||| |||| || || ||||||||||| |||||||| |||
Sbjct: 427 gtctatatatttactcccaatatacttaatgcaatcgaggatgtcttgaaacagaagaaa 486
Query: 119 gacagagcaaatttgnnnnnnnngtatctagcattgaagctcttcattcagcaactaagg 178
|| ||||| |||||| ||||| ||| ||||||||||||| || |||| ||||
Sbjct: 487 gatagagctaatttgcgccgt--gtatccagctttgaagctcttcagtccgcaatgaagg 544
Query: 179 cgcttccaccagactttgttaggttagatcaagacattttatcccctcgagcgggaaaga 238
| ||||| |||| | |||||||||||||||||| || ||||| |||| ||| |||||||
Sbjct: 545 caattccagcagattatgttaggttagatcaagatatcttatctcctctagcaggaaaga 604
Query: 239 aggagctgtacacataccagacacttgatttctgggaacagatcaaaaccccagggatgt 298
|||| || ||||||||| ||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 605 aggaactttacacatacgagacacttgatttttgggaacagatcaagactccagggatgt 664
Query: 299 ctttgagatgctctgg 314
||||||||||||||||
Sbjct: 665 ctttgagatgctctgg 680
>gb|AV837011.1|AV837011 AV837011 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd22m12, mRNA
sequence
Length = 531
Score = 85.7 bits (43), Expect = 3e-015
Identities = 80/91 (87%), Gaps = 1/91 (1%)
Strand = Plus / Plus
Query: 36 gtgagtgatctcataaattgcggaga-tatatatttactcccgatatccttagcgccatt 94
||||||||||||||||||||||||| ||||||||||||||| |||| |||| || ||
Sbjct: 141 gtgagtgatctcataaattgcggagtctatatatttactcccaatatacttaatgcaatc 200
Query: 95 gaggatgtcttaaaacagaaaaaagacagag 125
||||||||||| |||||||| ||||| ||||
Sbjct: 201 gaggatgtcttgaaacagaagaaagatagag 231
>gb|BJ467748.1|BJ467748 BJ467748 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29k09 3', mRNA sequence
Length = 264
Score = 63.9 bits (32), Expect = 9e-009
Identities = 47/52 (90%)
Strand = Plus / Minus
Query: 703 aataaaactctcaatgtcagtgtccaagaggagatcatcctataataaccat 754
|||||||||||||||| ||| || ||||| ||||||||||||||||| ||||
Sbjct: 264 aataaaactctcaatgccagcgtgcaagatgagatcatcctataatatccat 213
>gb|CA027638.1|CA027638 HZ59I23r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59I23
5-PRIME, mRNA sequence
Length = 368
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 525 tgaaattatggaaaatgctg 544
||||||||||||||||||||
Sbjct: 162 tgaaattatggaaaatgctg 181
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 83,000
Number of Sequences: 312970
Number of extensions: 83000
Number of successful extensions: 22814
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 22794
Number of HSP's gapped (non-prelim): 16
length of query: 1000
length of database: 175,134,539
effective HSP length: 19
effective length of query: 981
effective length of database: 169,188,109
effective search space: 165973534929
effective search space used: 165973534929
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)