BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440444.2.1
(237 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke devel... 68 1e-010
gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA li... 68 1e-010
gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA li... 68 1e-010
gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. v... 68 1e-010
gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. v... 68 1e-010
gb|BU971140.1|BU971140 HB16L15r BC Hordeum vulgare subsp. v... 36 0.48
>gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08G20u 3',
mRNA sequence
Length = 566
Score = 67.9 bits (34), Expect = 1e-010
Identities = 145/182 (79%)
Strand = Plus / Plus
Query: 56 atcgatcaccttcctgccatccgccctcaccctggagaaatgcttacacgggctgatgtc 115
||||||||||||| |||||||| ||||| ||||| || |||| || || |||| |||
Sbjct: 180 atcgatcaccttcttgccatcctccctcgccctgctaaagtgctcgcatggcctgacgtc 239
Query: 116 aaacctgagcttagccgtctcccctgccttgagatgctggctcctgaacccaatcagctg 175
||| |||| | ||| |||| || ||||||||||||||| | | |||||||||||||||
Sbjct: 240 gaacgtgaggctggccttctcgccggccttgagatgctgggttcggaacccaatcagctg 299
Query: 176 gcttgccgggcgaccttgtttcgtgtttggccagcggaggaacatgagcaccgagtgctt 235
||| |||| || || ||| ||| ||||| ||||||||||| ||||| ||||||||
Sbjct: 300 gctcaccggacgcccgcccgtcgcgttgggccaccggaggaacatcagcacagagtgctt 359
Query: 236 cc 237
||
Sbjct: 360 cc 361
>gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 5', mRNA sequence
Length = 582
Score = 67.9 bits (34), Expect = 1e-010
Identities = 145/182 (79%)
Strand = Plus / Minus
Query: 56 atcgatcaccttcctgccatccgccctcaccctggagaaatgcttacacgggctgatgtc 115
||||||||||||| |||||||| ||||| ||||| || |||| || || |||| |||
Sbjct: 488 atcgatcaccttcttgccatcctccctcgccctgctaaagtgctcgcatggcctgacgtc 429
Query: 116 aaacctgagcttagccgtctcccctgccttgagatgctggctcctgaacccaatcagctg 175
||| |||| | ||| |||| || ||||||||||||||| | | |||||||||||||||
Sbjct: 428 gaacgtgaggctggccttctcgccggccttgagatgctgggttcggaacccaatcagctg 369
Query: 176 gcttgccgggcgaccttgtttcgtgtttggccagcggaggaacatgagcaccgagtgctt 235
||| |||| || || ||| ||| ||||| ||||||||||| ||||| ||||||||
Sbjct: 368 gctcaccggacgcccgcccgtcgcgttgggccaccggaggaacatcagcacagagtgctt 309
Query: 236 cc 237
||
Sbjct: 308 cc 307
>gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 3', mRNA sequence
Length = 673
Score = 67.9 bits (34), Expect = 1e-010
Identities = 145/182 (79%)
Strand = Plus / Plus
Query: 56 atcgatcaccttcctgccatccgccctcaccctggagaaatgcttacacgggctgatgtc 115
||||||||||||| |||||||| ||||| ||||| || |||| || || |||| |||
Sbjct: 237 atcgatcaccttcttgccatcctccctcgccctgctaaagtgctcgcatggcctgacgtc 296
Query: 116 aaacctgagcttagccgtctcccctgccttgagatgctggctcctgaacccaatcagctg 175
||| |||| | ||| |||| || ||||||||||||||| | | |||||||||||||||
Sbjct: 297 gaacgtgaggctggccttctcgccggccttgagatgctgggttcggaacccaatcagctg 356
Query: 176 gcttgccgggcgaccttgtttcgtgtttggccagcggaggaacatgagcaccgagtgctt 235
||| |||| || || ||| ||| ||||| ||||||||||| ||||| ||||||||
Sbjct: 357 gctcaccggacgcccgcccgtcgcgttgggccaccggaggaacatcagcacagagtgctt 416
Query: 236 cc 237
||
Sbjct: 417 cc 418
>gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. vulgare cDNA clone HB21B09
5-PRIME, mRNA sequence
Length = 513
Score = 67.9 bits (34), Expect = 1e-010
Identities = 145/182 (79%)
Strand = Plus / Minus
Query: 56 atcgatcaccttcctgccatccgccctcaccctggagaaatgcttacacgggctgatgtc 115
||||||||||||| |||||||| ||||| ||||| || |||| || || |||| |||
Sbjct: 242 atcgatcaccttcttgccatcctccctcgccctgctaaagtgctcgcatggcctgacgtc 183
Query: 116 aaacctgagcttagccgtctcccctgccttgagatgctggctcctgaacccaatcagctg 175
||| |||| | ||| |||| || ||||||||||||||| | | |||||||||||||||
Sbjct: 182 gaacgtgaggctggccttctcgccggccttgagatgctgggttcggaacccaatcagctg 123
Query: 176 gcttgccgggcgaccttgtttcgtgtttggccagcggaggaacatgagcaccgagtgctt 235
||| |||| || || ||| ||| ||||| ||||||||||| ||||| ||||||||
Sbjct: 122 gctcaccggacgcccgcccgtcgcgttgggccaccggaggaacatcagcacagagtgctt 63
Query: 236 cc 237
||
Sbjct: 62 cc 61
>gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. vulgare cDNA clone HB21F19
5-PRIME, mRNA sequence
Length = 513
Score = 67.9 bits (34), Expect = 1e-010
Identities = 145/182 (79%)
Strand = Plus / Minus
Query: 56 atcgatcaccttcctgccatccgccctcaccctggagaaatgcttacacgggctgatgtc 115
||||||||||||| |||||||| ||||| ||||| || |||| || || |||| |||
Sbjct: 242 atcgatcaccttcttgccatcctccctcgccctgctaaagtgctcgcatggcctgacgtc 183
Query: 116 aaacctgagcttagccgtctcccctgccttgagatgctggctcctgaacccaatcagctg 175
||| |||| | ||| |||| || ||||||||||||||| | | |||||||||||||||
Sbjct: 182 gaacgtgaggctggccttctcgccggccttgagatgctgggttcggaacccaatcagctg 123
Query: 176 gcttgccgggcgaccttgtttcgtgtttggccagcggaggaacatgagcaccgagtgctt 235
||| |||| || || ||| ||| ||||| ||||||||||| ||||| ||||||||
Sbjct: 122 gctcaccggacgcccgcccgtcgcgttgggccaccggaggaacatcagcacagagtgctt 63
Query: 236 cc 237
||
Sbjct: 62 cc 61
>gb|BU971140.1|BU971140 HB16L15r BC Hordeum vulgare subsp. vulgare cDNA clone HB16L15
5-PRIME, mRNA sequence
Length = 445
Score = 36.2 bits (18), Expect = 0.48
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 198 gtgtttggccagcggagg 215
||||||||||||||||||
Sbjct: 380 gtgtttggccagcggagg 363
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 22,961
Number of Sequences: 312970
Number of extensions: 22961
Number of successful extensions: 5754
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5737
Number of HSP's gapped (non-prelim): 17
length of query: 237
length of database: 175,134,539
effective HSP length: 18
effective length of query: 219
effective length of database: 169,501,079
effective search space: 37120736301
effective search space used: 37120736301
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 18 (36.2 bits)