BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437629.2.2
(1041 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA li... 129 2e-028
gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA li... 129 2e-028
gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike... 113 1e-023
gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. v... 109 2e-022
gb|CV064134.1|CV064134 BNEL98d5 Barley EST endosperm librar... 88 7e-016
gb|CA010088.1|CA010088 HT12A16u HT Hordeum vulgare subsp. v... 74 1e-011
gb|AJ460855.1|AJ460855 AJ460855 S00002 Hordeum vulgare subs... 70 2e-010
gb|BI957363.1|BI957363 HVSMEn0009C19f Hordeum vulgare rachi... 48 6e-004
gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA li... 46 0.002
gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA li... 46 0.002
gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 D... 46 0.002
gb|CV064122.1|CV064122 BNEL98c5 Barley EST endosperm librar... 40 0.14
>gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 5', mRNA sequence
Length = 633
Score = 129 bits (65), Expect = 2e-028
Identities = 128/149 (85%)
Strand = Plus / Plus
Query: 7 ataattcgtcatccacgaattggtgaatatgcatttggattcataacatcaacggtggtc 66
|||||||| |||||||| |||||||||| ||||| ||||| ||||||||||| || ||
Sbjct: 467 ataattcgacatccacgtgttggtgaatacgcattcggatttataacatcaactgttgtt 526
Query: 67 cttcagactgacaaaggcgacgaagaactgtgtagtgtgtatgtgccaacaaatcatctg 126
|| ||||| |||||||| || || ||| |||||||||| |||||||| |||||||||||
Sbjct: 527 ctccagacagacaaaggtgatgaggaattgtgtagtgtttatgtgccgacaaatcatcta 586
Query: 127 tacatcggtgatatatttctggtgaactc 155
|| || ||||||||||||||| |||||||
Sbjct: 587 tatattggtgatatatttctgctgaactc 615
>gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 3', mRNA sequence
Length = 644
Score = 129 bits (65), Expect = 2e-028
Identities = 128/149 (85%)
Strand = Plus / Minus
Query: 7 ataattcgtcatccacgaattggtgaatatgcatttggattcataacatcaacggtggtc 66
|||||||| |||||||| |||||||||| ||||| ||||| ||||||||||| || ||
Sbjct: 527 ataattcgacatccacgtgttggtgaatacgcattcggatttataacatcaactgttgtt 468
Query: 67 cttcagactgacaaaggcgacgaagaactgtgtagtgtgtatgtgccaacaaatcatctg 126
|| ||||| |||||||| || || ||| |||||||||| |||||||| |||||||||||
Sbjct: 467 ctccagacagacaaaggtgatgaggaattgtgtagtgtttatgtgccgacaaatcatcta 408
Query: 127 tacatcggtgatatatttctggtgaactc 155
|| || ||||||||||||||| |||||||
Sbjct: 407 tatattggtgatatatttctgctgaactc 379
Score = 69.9 bits (35), Expect = 2e-010
Identities = 80/95 (84%)
Strand = Plus / Minus
Query: 396 gaaataattgtttcaggagggatgaccatgcctcaggtgattgcatcactggagcccatg 455
||||| || ||||| ||||| ||||||||||| ||||| |||||||| || || |||||
Sbjct: 335 gaaattatcgtttctggaggaatgaccatgccacaggtaattgcatcgctagaccccatc 276
Query: 456 ccacggaaaagccagaacattcgtctaaatagaat 490
||| |||| ||||||| ||| || |||||||||||
Sbjct: 275 ccaaggaagagccagagcatccgcctaaatagaat 241
>gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010L20f, mRNA sequence
Length = 834
Score = 113 bits (57), Expect = 1e-023
Identities = 111/129 (86%)
Strand = Plus / Plus
Query: 27 tggtgaatatgcatttggattcataacatcaacggtggtccttcagactgacaaaggcga 86
||||||||| ||||||||||| |||||||| | || | ||||||||||||||||| ||
Sbjct: 286 tggtgaatacgcatttggatttataacatcgagcatgattcttcagactgacaaaggtga 345
Query: 87 cgaagaactgtgtagtgtgtatgtgccaacaaatcatctgtacatcggtgatatatttct 146
||||||||||||||||| ||||||||||| |||||||| | || |||||||| ||| |
Sbjct: 346 tgaagaactgtgtagtgtctatgtgccaaccaatcatctatgtattggtgatatcttttt 405
Query: 147 ggtgaactc 155
|||||||||
Sbjct: 406 ggtgaactc 414
Score = 44.1 bits (22), Expect = 0.009
Identities = 34/38 (89%)
Strand = Plus / Plus
Query: 399 ataattgtttcaggagggatgaccatgcctcaggtgat 436
||||||||||| ||||| ||||||||||| |||||||
Sbjct: 461 ataattgtttctggaggaatgaccatgccggaggtgat 498
>gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. vulgare cDNA clone HI07G08
3-PRIME, mRNA sequence
Length = 580
Score = 109 bits (55), Expect = 2e-022
Identities = 103/119 (86%)
Strand = Plus / Minus
Query: 37 gcatttggattcataacatcaacggtggtccttcagactgacaaaggcgacgaagaactg 96
||||||||||| |||||||| | || | ||||||||||||||||| || |||||||||
Sbjct: 578 gcatttggatttataacatcgagcatgattcttcagactgacaaaggtgatgaagaactg 519
Query: 97 tgtagtgtgtatgtgccaacaaatcatctgtacatcggtgatatatttctggtgaactc 155
|||||||| ||||||||||| |||||||| || || |||||||| ||| ||||||||||
Sbjct: 518 tgtagtgtctatgtgccaaccaatcatctatatattggtgatatctttttggtgaactc 460
Score = 60.0 bits (30), Expect = 2e-007
Identities = 39/42 (92%)
Strand = Plus / Minus
Query: 395 agaaataattgtttcaggagggatgaccatgcctcaggtgat 436
||||||||||||||| ||||| ||||||||||| ||||||||
Sbjct: 417 agaaataattgtttctggaggaatgaccatgccgcaggtgat 376
>gb|CV064134.1|CV064134 BNEL98d5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98d5 5' similar to unknown
protein, mRNA sequence
Length = 649
Score = 87.7 bits (44), Expect = 7e-016
Identities = 77/88 (87%)
Strand = Plus / Plus
Query: 27 tggtgaatatgcatttggattcataacatcaacggtggtccttcagactgacaaaggcga 86
||||||||| ||||||||||| |||||||| | || | ||||||||||||||||| ||
Sbjct: 562 tggtgaatacgcatttggatttataacatcgagcatgattcttcagactgacaaaggtga 621
Query: 87 cgaagaactgtgtagtgtgtatgtgcca 114
||||||||||||||||| |||||||||
Sbjct: 622 tgaagaactgtgtagtgtctatgtgcca 649
>gb|CA010088.1|CA010088 HT12A16u HT Hordeum vulgare subsp. vulgare cDNA clone HT12A16
3-PRIME, mRNA sequence
Length = 536
Score = 73.8 bits (37), Expect = 1e-011
Identities = 82/97 (84%)
Strand = Plus / Minus
Query: 394 cagaaataattgtttcaggagggatgaccatgcctcaggtgattgcatcactggagccca 453
||||||| || ||||| ||||| ||||||||||| ||||| |||||||| || || ||||
Sbjct: 512 cagaaattatcgtttctggaggaatgaccatgccacaggtaattgcatcgctagacccca 453
Query: 454 tgccacggaaaagccagaacattcgtctaaatagaat 490
| ||| |||| ||||||| ||| || |||||||||||
Sbjct: 452 tcccaaggaagagccagagcatccgcctaaatagaat 416
>gb|AJ460855.1|AJ460855 AJ460855 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200071B10F1, mRNA sequence
Length = 240
Score = 69.9 bits (35), Expect = 2e-010
Identities = 80/95 (84%)
Strand = Plus / Plus
Query: 396 gaaataattgtttcaggagggatgaccatgcctcaggtgattgcatcactggagcccatg 455
||||| || ||||| ||||| ||||||||||| ||||| |||||||| || || |||||
Sbjct: 41 gaaattatcgtttctggaggaatgaccatgccacaggtaattgcatcgctagaccccatc 100
Query: 456 ccacggaaaagccagaacattcgtctaaatagaat 490
||| |||| ||||||| ||| || |||||||||||
Sbjct: 101 ccaaggaagagccagagcatccgcctaaatagaat 135
>gb|BI957363.1|BI957363 HVSMEn0009C19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009C19f, mRNA sequence
Length = 656
Score = 48.1 bits (24), Expect = 6e-004
Identities = 64/76 (84%), Gaps = 1/76 (1%)
Strand = Plus / Plus
Query: 27 tggtgaatatgcatttggattcataacatcaacgg-tggtccttcagactgacaaaggcg 85
||||||||| ||||||||||| |||||||| | || | ||||||||||||||| | |
Sbjct: 581 tggtgaatacgcatttggatttataacatcggagcatgattcttcagactgacaaaagtg 640
Query: 86 acgaagaactgtgtag 101
| ||||||||||||||
Sbjct: 641 atgaagaactgtgtag 656
>gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35h23 3', mRNA sequence
Length = 697
Score = 46.1 bits (23), Expect = 0.002
Identities = 32/35 (91%)
Strand = Plus / Minus
Query: 16 catccacgaattggtgaatatgcatttggattcat 50
||||| || ||||| ||||||||||||||||||||
Sbjct: 459 catcctcgtattggggaatatgcatttggattcat 425
>gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15k07 3', mRNA sequence
Length = 699
Score = 46.1 bits (23), Expect = 0.002
Identities = 32/35 (91%)
Strand = Plus / Minus
Query: 16 catccacgaattggtgaatatgcatttggattcat 50
||||| || ||||| ||||||||||||||||||||
Sbjct: 459 catcctcgtattggggaatatgcatttggattcat 425
>gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ001_H19 5', mRNA sequence
Length = 527
Score = 46.1 bits (23), Expect = 0.002
Identities = 32/35 (91%)
Strand = Plus / Plus
Query: 16 catccacgaattggtgaatatgcatttggattcat 50
||||| || ||||| ||||||||||||||||||||
Sbjct: 311 catcctcgtattggggaatatgcatttggattcat 345
>gb|CV064122.1|CV064122 BNEL98c5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98c5 5' similar to Oryza sativa,
mRNA sequence
Length = 648
Score = 40.1 bits (20), Expect = 0.14
Identities = 41/48 (85%)
Strand = Plus / Plus
Query: 67 cttcagactgacaaaggcgacgaagaactgtgtagtgtgtatgtgcca 114
||||| ||||||||||| || ||| |||||||||| || |||| ||||
Sbjct: 601 cttcacactgacaaaggtgatgaacaactgtgtagcgtctatgagcca 648
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 78,052
Number of Sequences: 312970
Number of extensions: 78052
Number of successful extensions: 21961
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 21935
Number of HSP's gapped (non-prelim): 23
length of query: 1041
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1022
effective length of database: 169,188,109
effective search space: 172910247398
effective search space used: 172910247398
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)