BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404863.2.1
(672 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA li... 242 1e-062
gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA li... 242 1e-062
gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA li... 242 1e-062
gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. v... 242 1e-062
gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no... 159 1e-037
gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, n... 145 2e-033
gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm libr... 72 3e-011
gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm librar... 72 3e-011
gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm librar... 72 3e-011
gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm librar... 72 3e-011
gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm librar... 72 3e-011
gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. v... 64 6e-009
gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. v... 64 6e-009
gb|BM371651.1|BM371651 EBma08_SQ003_I01_R maternal, 28 DPA,... 44 0.006
gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. v... 44 0.006
gb|BQ659745.1|BQ659745 HF01J09w HF Hordeum vulgare subsp. v... 38 0.36
>gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdd19 5', mRNA sequence
Length = 612
Score = 242 bits (122), Expect = 1e-062
Identities = 227/262 (86%)
Strand = Plus / Plus
Query: 1 ggacctggatgtgtcgtggaggatggcgtgaggctttcccgctgcactgtcatgcgtggt 60
||||||||||| || ||||||||||| |||||||| ||||| ||||| || |||||||||
Sbjct: 316 ggacctggatgcgttgtggaggatggtgtgaggctctcccgatgcacggtgatgcgtggt 375
Query: 61 gtgcgtatcaagaagcatgcctgcatctcgaacagcattatcgggtggcactcaactgtt 120
|| || || ||||||||||| |||||||| |||||||| ||||| ||||| || || ||
Sbjct: 376 gtacgcattaagaagcatgcgtgcatctcaaacagcatcatcggctggcattcgacggtc 435
Query: 121 ggacaatgggcacggatagagaatatgaccatcctgggcgaggatgttcatgggggtgac 180
|| ||||||||||| |||||||||||||| |||||||| || ||||| |||| ||||||
Sbjct: 436 gggcaatgggcacgcatagagaatatgactatcctgggagaagatgtacatgttggtgac 495
Query: 181 gaggtatacagcaatggcggtgttgttctcccacagaaagagatcaagtcaagcattttg 240
||||| |||||||| |||||||||||||| || || || ||||||||||||||||| |
Sbjct: 496 gaggtctacagcaacggcggtgttgttctgccgcacaaggagatcaagtcaagcatcctt 555
Query: 241 aagcctgagatcgtcatgtgag 262
||||||||||||||||||||||
Sbjct: 556 aagcctgagatcgtcatgtgag 577
>gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd15d19 3', mRNA sequence
Length = 673
Score = 242 bits (122), Expect = 1e-062
Identities = 227/262 (86%)
Strand = Plus / Minus
Query: 1 ggacctggatgtgtcgtggaggatggcgtgaggctttcccgctgcactgtcatgcgtggt 60
||||||||||| || ||||||||||| |||||||| ||||| ||||| || |||||||||
Sbjct: 367 ggacctggatgcgttgtggaggatggtgtgaggctctcccgatgcacggtgatgcgtggt 308
Query: 61 gtgcgtatcaagaagcatgcctgcatctcgaacagcattatcgggtggcactcaactgtt 120
|| || || ||||||||||| |||||||| |||||||| ||||| ||||| || || ||
Sbjct: 307 gtacgcattaagaagcatgcgtgcatctcaaacagcatcatcggctggcattcgacggtc 248
Query: 121 ggacaatgggcacggatagagaatatgaccatcctgggcgaggatgttcatgggggtgac 180
|| ||||||||||| |||||||||||||| |||||||| || ||||| |||| ||||||
Sbjct: 247 gggcaatgggcacgcatagagaatatgactatcctgggagaagatgtacatgttggtgac 188
Query: 181 gaggtatacagcaatggcggtgttgttctcccacagaaagagatcaagtcaagcattttg 240
||||| |||||||| |||||||||||||| || || || ||||||||||||||||| |
Sbjct: 187 gaggtctacagcaacggcggtgttgttctgccgcacaaggagatcaagtcaagcatcctt 128
Query: 241 aagcctgagatcgtcatgtgag 262
||||||||||||||||||||||
Sbjct: 127 aagcctgagatcgtcatgtgag 106
>gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6n22 3', mRNA sequence
Length = 651
Score = 242 bits (122), Expect = 1e-062
Identities = 227/262 (86%)
Strand = Plus / Minus
Query: 1 ggacctggatgtgtcgtggaggatggcgtgaggctttcccgctgcactgtcatgcgtggt 60
||||||||||| || ||||||||||| |||||||| ||||| ||||| || |||||||||
Sbjct: 335 ggacctggatgcgttgtggaggatggtgtgaggctctcccgatgcacggtgatgcgtggt 276
Query: 61 gtgcgtatcaagaagcatgcctgcatctcgaacagcattatcgggtggcactcaactgtt 120
|| || || ||||||||||| |||||||| |||||||| ||||| ||||| || || ||
Sbjct: 275 gtacgcattaagaagcatgcgtgcatctcaaacagcatcatcggctggcattcgacggtc 216
Query: 121 ggacaatgggcacggatagagaatatgaccatcctgggcgaggatgttcatgggggtgac 180
|| ||||||||||| |||||||||||||| |||||||| || ||||| |||| ||||||
Sbjct: 215 gggcaatgggcacgcatagagaatatgactatcctgggagaagatgtacatgttggtgac 156
Query: 181 gaggtatacagcaatggcggtgttgttctcccacagaaagagatcaagtcaagcattttg 240
||||| |||||||| |||||||||||||| || || || ||||||||||||||||| |
Sbjct: 155 gaggtctacagcaacggcggtgttgttctgccgcacaaggagatcaagtcaagcatcctt 96
Query: 241 aagcctgagatcgtcatgtgag 262
||||||||||||||||||||||
Sbjct: 95 aagcctgagatcgtcatgtgag 74
>gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. vulgare cDNA clone HA03D09
3-PRIME, mRNA sequence
Length = 485
Score = 242 bits (122), Expect = 1e-062
Identities = 227/262 (86%)
Strand = Plus / Minus
Query: 1 ggacctggatgtgtcgtggaggatggcgtgaggctttcccgctgcactgtcatgcgtggt 60
||||||||||| || ||||||||||| |||||||| ||||| ||||| || |||||||||
Sbjct: 440 ggacctggatgcgttgtggaggatggtgtgaggctctcccgatgcacggtgatgcgtggt 381
Query: 61 gtgcgtatcaagaagcatgcctgcatctcgaacagcattatcgggtggcactcaactgtt 120
|| || || ||||||||||| |||||||| |||||||| ||||| ||||| || || ||
Sbjct: 380 gtacgcattaagaagcatgcgtgcatctcaaacagcatcatcggctggcattcgacggtc 321
Query: 121 ggacaatgggcacggatagagaatatgaccatcctgggcgaggatgttcatgggggtgac 180
|| ||||||||||| |||||||||||||| |||||||| || ||||| |||| ||||||
Sbjct: 320 gggcaatgggcacgcatagagaatatgactatcctgggagaagatgtacatgttggtgac 261
Query: 181 gaggtatacagcaatggcggtgttgttctcccacagaaagagatcaagtcaagcattttg 240
||||| |||||||| |||||||||||||| || || || ||||||||||||||||| |
Sbjct: 260 gaggtctacagcaacggcggtgttgttctgccgcacaaggagatcaagtcaagcatcctt 201
Query: 241 aagcctgagatcgtcatgtgag 262
||||||||||||||||||||||
Sbjct: 200 aagcctgagatcgtcatgtgag 179
>gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ001_M01 5', mRNA sequence
Length = 245
Score = 159 bits (80), Expect = 1e-037
Identities = 179/211 (84%), Gaps = 2/211 (0%)
Strand = Plus / Plus
Query: 52 atgcgtggtgtgcgtatcaagaagcatgcctgcatctcgaacagcattatcgggtggcac 111
||||||||||| || || ||||||||||| |||||| | ||||||| ||||| |||||
Sbjct: 5 atgcgtggtgtacgcattaagaagcatgcgtgcatcggggacagcatcatcggctggcat 64
Query: 112 tcaactgttggacaatgggcacggatagagaatatgaccatcctgggcgaggatgttcat 171
|| || || || ||||||||||| ||||||||| ||| |||||||| || ||||| |||
Sbjct: 65 tcgacggtcgggcaatgggcacgcatagagaat--gactatcctgggagaagatgtacat 122
Query: 172 gggggtgacgaggtatacagcaatggcggtgttgttctcccacagaaagagatcaagtca 231
| ||||||||||| |||||||| |||||||||||||| || || || ||||||||||||
Sbjct: 123 gttggtgacgaggtctacagcaacggcggtgttgttctgccgcacaaggagatcaagtca 182
Query: 232 agcattttgaagcctgagatcgtcatgtgag 262
||||| | ||||||||||||||||||||||
Sbjct: 183 agcatccttaagcctgagatcgtcatgtgag 213
>gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_K16 5', mRNA sequence
Length = 357
Score = 145 bits (73), Expect = 2e-033
Identities = 155/181 (85%), Gaps = 1/181 (0%)
Strand = Plus / Plus
Query: 82 tgcatctcgaacagcattatcgggtggcactcaactgttggacaatgggcacggatagag 141
|||||||| |||||||| ||||| ||||| || || || || ||||||||||| ||||||
Sbjct: 2 tgcatctcaaacagcatcatcggctggcattcgacggtcgggcaatgggcacgcatagag 61
Query: 142 aatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggcggt 201
|||||||| |||||||| || |||| |||| ||||||||||| |||||||| ||||||
Sbjct: 62 aatatgactatcctgggaaag-atgtacatgttggtgacgaggtctacagcaacggcggt 120
Query: 202 gttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatgtga 261
|||||| | || || || ||||||||||||||||| | |||||||||||||||||||||
Sbjct: 121 gttgttttgccgcacaaggagatcaagtcaagcatccttaagcctgagatcgtcatgtga 180
Query: 262 g 262
|
Sbjct: 181 g 181
>gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL120h12 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 730
Score = 71.9 bits (36), Expect = 3e-011
Identities = 102/124 (82%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 431 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 372
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||||||||||
Sbjct: 371 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagatcgtcatg 312
Query: 259 tgag 262
||||
Sbjct: 311 tgag 308
>gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL22a9 5' similar to GDP-D-mannose
pyrophosphorylase, mRNA sequence
Length = 555
Score = 71.9 bits (36), Expect = 3e-011
Identities = 102/124 (82%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 431 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 372
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||||||||||
Sbjct: 371 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagatcgtcatg 312
Query: 259 tgag 262
||||
Sbjct: 311 tgag 308
>gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL43h6 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 500
Score = 71.9 bits (36), Expect = 3e-011
Identities = 102/124 (82%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 426 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 367
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||||||||||
Sbjct: 366 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagatcgtcatg 307
Query: 259 tgag 262
||||
Sbjct: 306 tgag 303
>gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL46d8 5' similar to COG1208, GCD1,
Nucleoside-diphosphate-sugar pyrophosphorylase involved
in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 637
Score = 71.9 bits (36), Expect = 3e-011
Identities = 102/124 (82%)
Strand = Plus / Plus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 503 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 562
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||||||||||
Sbjct: 563 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagatcgtcatg 622
Query: 259 tgag 262
||||
Sbjct: 623 tgag 626
>gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL83e1 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 595
Score = 71.9 bits (36), Expect = 3e-011
Identities = 102/124 (82%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 422 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 363
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||||||||||
Sbjct: 362 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagatcgtcatg 303
Query: 259 tgag 262
||||
Sbjct: 302 tgag 299
>gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. vulgare cDNA clone HA08H22
3-PRIME, mRNA sequence
Length = 497
Score = 63.9 bits (32), Expect = 6e-009
Identities = 101/124 (81%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 412 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 353
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||| ||||||
Sbjct: 352 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagattgtcatg 293
Query: 259 tgag 262
||||
Sbjct: 292 tgag 289
>gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. vulgare cDNA clone HF06A23
5-PRIME, mRNA sequence
Length = 455
Score = 63.9 bits (32), Expect = 6e-009
Identities = 101/124 (81%)
Strand = Plus / Minus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 410 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 351
Query: 199 ggtgttgttctcccacagaaagagatcaagtcaagcattttgaagcctgagatcgtcatg 258
|| || || ||||| || || ||||||||||| ||||| | ||||| ||||| ||||||
Sbjct: 350 ggcgtcgtgctcccgcacaaggagatcaagtccagcatcctcaagcccgagattgtcatg 291
Query: 259 tgag 262
||||
Sbjct: 290 tgag 287
>gb|BM371651.1|BM371651 EBma08_SQ003_I01_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ003_I01 5', mRNA sequence
Length = 173
Score = 44.1 bits (22), Expect = 0.006
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 241 aagcctgagatcgtcatgtgag 262
||||||||||||||||||||||
Sbjct: 9 aagcctgagatcgtcatgtgag 30
>gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. vulgare cDNA clone HX11E06
5-PRIME, mRNA sequence
Length = 620
Score = 44.1 bits (22), Expect = 0.006
Identities = 73/90 (81%)
Strand = Plus / Plus
Query: 139 gagaatatgaccatcctgggcgaggatgttcatgggggtgacgaggtatacagcaatggc 198
||||| ||||||||||| |||||||| || || | | |||||||| |||||||| |||
Sbjct: 531 gagaacatgaccatcctcggcgaggacgtgcacgtctgcgacgaggtgtacagcaacggc 590
Query: 199 ggtgttgttctcccacagaaagagatcaag 228
|| || || ||||| || || |||||||||
Sbjct: 591 ggcgtcgtgctcccgcacaaggagatcaag 620
>gb|BQ659745.1|BQ659745 HF01J09w HF Hordeum vulgare subsp. vulgare cDNA clone HF01J09
3-PRIME, mRNA sequence
Length = 601
Score = 38.2 bits (19), Expect = 0.36
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 413 cttgttctttgttgaaaag 431
|||||||||||||||||||
Sbjct: 19 cttgttctttgttgaaaag 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 50,790
Number of Sequences: 312970
Number of extensions: 50790
Number of successful extensions: 14453
Number of sequences better than 0.5: 16
Number of HSP's better than 0.5 without gapping: 15
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 14422
Number of HSP's gapped (non-prelim): 23
length of query: 672
length of database: 175,134,539
effective HSP length: 19
effective length of query: 653
effective length of database: 169,188,109
effective search space: 110479835177
effective search space used: 110479835177
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)