BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276425.2.1
(753 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ458898.1|BQ458898 HA02O06r HA Hordeum vulgare subsp. v... 133 9e-030
gb|BQ656596.1|BQ656596 HA02O06u HA Hordeum vulgare subsp. v... 133 9e-030
gb|BQ660593.1|BQ660593 HI04G01u HI Hordeum vulgare subsp. v... 90 1e-016
gb|AV915249.1|AV915249 AV915249 K. Sato unpublished cDNA li... 70 1e-010
gb|AV921857.1|AV921857 AV921857 K. Sato unpublished cDNA li... 70 1e-010
gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA li... 70 1e-010
gb|BJ465852.1|BJ465852 BJ465852 K. Sato unpublished cDNA li... 62 3e-008
gb|BJ459802.1|BJ459802 BJ459802 K. Sato unpublished cDNA li... 46 0.002
gb|AJ462461.1|AJ462461 AJ462461 S00002 Hordeum vulgare subs... 44 0.007
>gb|BQ458898.1|BQ458898 HA02O06r HA Hordeum vulgare subsp. vulgare cDNA clone HA02O06
5-PRIME, mRNA sequence
Length = 443
Score = 133 bits (67), Expect = 9e-030
Identities = 172/207 (83%)
Strand = Plus / Plus
Query: 194 tttggagactaccctgtcgagcgatgcctcaagaatatggaccgtgcattcaattttggg 253
||||| || |||||||| ||| ||||||||||| | ||||||||||| |||||||| |||
Sbjct: 19 tttggggattaccctgttgagagatgcctcaagcagatggaccgtgccttcaatttcggg 78
Query: 254 gataaccagatcttgcagatgtatgggttcacacacaagtcactagcaagcaggagagtg 313
|| |||||| ||||||| ||||||||||| |||||||| || || |||||||| |
Sbjct: 79 gacaaccaggtcttgcacatgtatgggtttgagcacaagtctcttgcgagcaggaggatc 138
Query: 314 aagaggatcaggaacgagaccagcaacccacttgagacaaaggatgagcttgggttgctt 373
|||||||||||||| |||||||| ||||| ||||| | |||||||| ||||||||
Sbjct: 139 aagaggatcaggaatgagaccagtaaccctcttgatatgaaggatgattacgggttgctc 198
Query: 374 catccagcattcaaggctgtgaagact 400
|| |||||||||||||| || ||||||
Sbjct: 199 cacccagcattcaaggccgtcaagact 225
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 528 gggatggatatctttgttgtaatgtga 554
||||||||||||| ||||||| |||||
Sbjct: 362 gggatggatatctatgttgtactgtga 388
>gb|BQ656596.1|BQ656596 HA02O06u HA Hordeum vulgare subsp. vulgare cDNA clone HA02O06
3-PRIME, mRNA sequence
Length = 443
Score = 133 bits (67), Expect = 9e-030
Identities = 172/207 (83%)
Strand = Plus / Minus
Query: 194 tttggagactaccctgtcgagcgatgcctcaagaatatggaccgtgcattcaattttggg 253
||||| || |||||||| ||| ||||||||||| | ||||||||||| |||||||| |||
Sbjct: 425 tttggggattaccctgttgagagatgcctcaagcagatggaccgtgccttcaatttcggg 366
Query: 254 gataaccagatcttgcagatgtatgggttcacacacaagtcactagcaagcaggagagtg 313
|| |||||| ||||||| ||||||||||| |||||||| || || |||||||| |
Sbjct: 365 gacaaccaggtcttgcacatgtatgggtttgagcacaagtctcttgcgagcaggaggatc 306
Query: 314 aagaggatcaggaacgagaccagcaacccacttgagacaaaggatgagcttgggttgctt 373
|||||||||||||| |||||||| ||||| ||||| | |||||||| ||||||||
Sbjct: 305 aagaggatcaggaatgagaccagtaaccctcttgatatgaaggatgattacgggttgctc 246
Query: 374 catccagcattcaaggctgtgaagact 400
|| |||||||||||||| || ||||||
Sbjct: 245 cacccagcattcaaggccgtcaagact 219
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Minus
Query: 528 gggatggatatctttgttgtaatgtga 554
||||||||||||| ||||||| |||||
Sbjct: 82 gggatggatatctatgttgtactgtga 56
>gb|BQ660593.1|BQ660593 HI04G01u HI Hordeum vulgare subsp. vulgare cDNA clone HI04G01
3-PRIME, mRNA sequence
Length = 507
Score = 89.7 bits (45), Expect = 1e-016
Identities = 129/157 (82%)
Strand = Plus / Minus
Query: 244 caattttggggataaccagatcttgcagatgtatgggttcacacacaagtcactagcaag 303
|||||| ||||| |||||| ||||||| ||||||||||| |||||||| || || ||
Sbjct: 507 caatttcggggacaaccaggtcttgcacatgtatgggtttgagcacaagtctcttgcgag 448
Query: 304 caggagagtgaagaggatcaggaacgagaccagcaacccacttgagacaaaggatgagct 363
|||||| | |||||||||||||| |||||||| ||||| ||||| | ||||||||
Sbjct: 447 caggaggatcaagaggatcaggaatgagaccagtaaccctcttgatatgaaggatgatta 388
Query: 364 tgggttgcttcatccagcattcaaggctgtgaagact 400
|||||||| || |||||||||||||| || ||||||
Sbjct: 387 cgggttgctccacccagcattcaaggccgtcaagact 351
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Minus
Query: 528 gggatggatatctttgttgtaatgtga 554
||||||||||||| ||||||| |||||
Sbjct: 214 gggatggatatctatgttgtactgtga 188
>gb|AV915249.1|AV915249 AV915249 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13i20 5', mRNA sequence
Length = 579
Score = 69.9 bits (35), Expect = 1e-010
Identities = 80/95 (84%)
Strand = Plus / Plus
Query: 89 ttggtcgacaggtacgaggagatgcttgagaattacaagccagggctcggcgatcaccgg 148
||||| |||| ||||||||||||| | ||||| || | |||||||| || ||| | |||
Sbjct: 102 ttggtggacaagtacgaggagatgatggagaaccaccatccagggcttggggatgagcgg 161
Query: 149 tggccactcgtcacacactttgtcgggtgcaagcc 183
|||||| | || |||||||||||||||||||||||
Sbjct: 162 tggccatttgtgacacactttgtcgggtgcaagcc 196
>gb|AV921857.1|AV921857 AV921857 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13i20 3', mRNA sequence
Length = 664
Score = 69.9 bits (35), Expect = 1e-010
Identities = 80/95 (84%)
Strand = Plus / Minus
Query: 89 ttggtcgacaggtacgaggagatgcttgagaattacaagccagggctcggcgatcaccgg 148
||||| |||| ||||||||||||| | ||||| || | |||||||| || ||| | |||
Sbjct: 614 ttggtggacaagtacgaggagatgatggagaaccaccatccagggcttggggatgagcgg 555
Query: 149 tggccactcgtcacacactttgtcgggtgcaagcc 183
|||||| | || |||||||||||||||||||||||
Sbjct: 554 tggccatttgtgacacactttgtcgggtgcaagcc 520
>gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i03 3', mRNA sequence
Length = 653
Score = 69.9 bits (35), Expect = 1e-010
Identities = 80/95 (84%)
Strand = Plus / Minus
Query: 89 ttggtcgacaggtacgaggagatgcttgagaattacaagccagggctcggcgatcaccgg 148
||||| |||| ||||||||||||| | ||||| || | |||||||| || ||| | |||
Sbjct: 504 ttggtggacaagtacgaggagatgatggagaaccaccatccagggcttggggatgagcgg 445
Query: 149 tggccactcgtcacacactttgtcgggtgcaagcc 183
|||||| | || |||||||||||||||||||||||
Sbjct: 444 tggccatttgtgacacactttgtcgggtgcaagcc 410
>gb|BJ465852.1|BJ465852 BJ465852 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags27j24 3', mRNA sequence
Length = 504
Score = 61.9 bits (31), Expect = 3e-008
Identities = 73/87 (83%)
Strand = Plus / Minus
Query: 314 aagaggatcaggaacgagaccagcaacccacttgagacaaaggatgagcttgggttgctt 373
|||||||||||||| |||||||| ||||| ||||| | |||||||| ||||||||
Sbjct: 499 aagaggatcaggaatgagaccagtaaccctcttgatatgaaggatgattacgggttgctc 440
Query: 374 catccagcattcaaggctgtgaagact 400
|| |||||||||||||| || ||||||
Sbjct: 439 cacccagcattcaaggccgtcaagact 413
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Minus
Query: 528 gggatggatatctttgttgtaatgtga 554
||||||||||||| ||||||| |||||
Sbjct: 276 gggatggatatctatgttgtactgtga 250
>gb|BJ459802.1|BJ459802 BJ459802 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38f08 3', mRNA sequence
Length = 606
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 161 acacactttgtcgggtgcaagcc 183
|||||||||||||||||||||||
Sbjct: 603 acacactttgtcgggtgcaagcc 581
>gb|AJ462461.1|AJ462461 AJ462461 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200052A08F1, mRNA sequence
Length = 360
Score = 44.1 bits (22), Expect = 0.007
Identities = 76/94 (80%)
Strand = Plus / Plus
Query: 90 tggtcgacaggtacgaggagatgcttgagaattacaagccagggctcggcgatcaccggt 149
|||| |||| ||||||||||||| | ||||| || | || ||||||||||| | ||||
Sbjct: 3 tggtggacaagtacgaggagatgatggagaagcaccacccggggctcggcgacgagcggt 62
Query: 150 ggccactcgtcacacactttgtcgggtgcaagcc 183
|||| || |||| ||||| ||||| ||||||||
Sbjct: 63 ggcccttcatcacgcacttcgtcggatgcaagcc 96
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 52,780
Number of Sequences: 312970
Number of extensions: 52780
Number of successful extensions: 13924
Number of sequences better than 0.5: 9
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13892
Number of HSP's gapped (non-prelim): 25
length of query: 753
length of database: 175,134,539
effective HSP length: 19
effective length of query: 734
effective length of database: 169,188,109
effective search space: 124184072006
effective search space used: 124184072006
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)