BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192679.2.1
(1274 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE602597.3|BE602597 HVSMEh0100E21f Hordeum vulgare 5-45 ... 323 8e-087
gb|AL507344.1|AL507344 AL507344 Hordeum vulgare Barke devel... 313 8e-084
gb|BI776636.2|BI776636 EBpi07_SQ001_D15_R pistil, 12 DPA, n... 165 4e-039
gb|BM101281.2|BM101281 EBpi01_SQ003_G14_R pistil, 1 DPA, no... 44 0.011
gb|BU973701.1|BU973701 HB25L01r BC Hordeum vulgare subsp. v... 44 0.011
gb|CK124325.1|CK124325 BES1824108g01 BES1824 Hordeum vulgar... 42 0.044
gb|BG343405.2|BG343405 HVSMEg0005K17f Hordeum vulgare pre-a... 40 0.18
gb|CA019628.1|CA019628 HV12J16r HV Hordeum vulgare subsp. v... 40 0.18
>gb|BE602597.3|BE602597 HVSMEh0100E21f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0100E21f, mRNA sequence
Length = 732
Score = 323 bits (163), Expect = 8e-087
Identities = 408/489 (83%), Gaps = 3/489 (0%)
Strand = Plus / Plus
Query: 103 cttctcgccgtcgcagttcacctcctcccagaatgccgccgccgactccaccacgccttc 162
|||||||||||||||||||||| | || ||||| |||||||||||||||||| ||
Sbjct: 90 cttctcgccgtcgcagttcaccgcgtcgcagaacgccgccgccgactccacc---cccaa 146
Query: 163 caagatgcgcggcgcgtccagcaccatgccgctcaccgtgaagcaggtcgtcgacgcgca 222
|||| | || |||||||| |||| ||||||||||||||||||||| ||| ||||||||
Sbjct: 147 caagttccggggcgcgtcgggcacgatgccgctcaccgtgaagcagatcgcggacgcgca 206
Query: 223 gcagtctggcacgggcgacaagggcgctccgttcatcgtcaatggcgtcgagatggctaa 282
|| | | ||||| ||||| ||||||| || ||| ||||| | |||||||||| ||| ||
Sbjct: 207 gctggccggcaccggcgagaagggcgggcccttcgtcgtcgacggcgtcgagacggccaa 266
Query: 283 cattcgacttgtggggatggtcaatgccaaggtggagcggacgaccgatgtgaccttcac 342
| | | || ||||||||||| | | |||||||||||||| || || ||| |||||||
Sbjct: 267 cgtcaggctcgtggggatggtgagcggcaaggtggagcggaacacggacgtgtccttcac 326
Query: 343 gctcgacgatggcaccggccgcctcgatttcatcagatgggtgaatgatgcttcagattc 402
|||||||| |||||||||||| |||| |||||||||||||||||||||||| |||| ||
Sbjct: 327 cctcgacgacggcaccggccgcatcgacttcatcagatgggtgaatgatgctgcagactc 386
Query: 403 ttttgaaactgctgctattcagaatggtatgtacattgcggtcattggaagcctcaaggg 462
| ||||||||||||||||||||| |||||||| |||| |||||||| |||||||||||
Sbjct: 387 tgctgaaactgctgctattcagaacggtatgtatgttgccgtcattgggagcctcaaggg 446
Query: 463 actgcaagagaggaagcgtgctactgctttctcaatcaggcctataaccgatttcaatga 522
| | || || | |||||||||||||||||| || |||| |||||||| |||| ||| ||
Sbjct: 447 agttcaggacaagaagcgtgctactgcttttgcagtcagacctataactgattacaacga 506
Query: 523 ggttacgctgcatttcattcagtgtgttcggatgcatatagagaacattgaattaaaggc 582
||| || |||||||||||||||||||| ||||||||| |||||||| ||| |||||
Sbjct: 507 ggtcaccctgcatttcattcagtgtgtacggatgcatgcagagaacacaaaatcaaaggt 566
Query: 583 tggcagtcc 591
|||||||||
Sbjct: 567 tggcagtcc 575
>gb|AL507344.1|AL507344 AL507344 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05B07V 5',
mRNA sequence
Length = 659
Score = 313 bits (158), Expect = 8e-084
Identities = 392/468 (83%), Gaps = 4/468 (0%)
Strand = Plus / Plus
Query: 103 cttctcgccgtcgcagttcacctcctcccagaatgccgccgccgactccaccacgccttc 162
|||||||||||||||||||||| | || ||||| |||||||||||||||||| ||
Sbjct: 99 cttctcgccgtcgcagttcaccgcgtcgcagaacgccgccgccgactccacc---cccaa 155
Query: 163 caagatgcgcggcgcgtccagcaccatgccgctcaccgtgaagcaggtcgtcgacgcgca 222
|||| | || |||||||| |||| ||||||||||||||||||||| ||| ||||||||
Sbjct: 156 caagttccggggcgcgtcgggcacgatgccgctcaccgtgaagcagatcgcggacgcgca 215
Query: 223 gcagtctggcacgggcgacaagggcgctccgttcatcgtcaatggcgtcgagatggctaa 282
|| | | ||||| ||||| ||||||| || ||| ||||| | |||||||||| ||| ||
Sbjct: 216 gctggccggcaccggcgagaagggcgggcccttcgtcgtcgacggcgtcgagacggccaa 275
Query: 283 cattcgacttgtggggatggtcaatgccaaggtggagcggacgaccgatgtgaccttcac 342
| | | || ||||||||||| | | |||||||||||||| || || ||| |||||||
Sbjct: 276 cgtcaggctcgtggggatggtgagcggcaaggtggagcggaacacggacgtgtccttcac 335
Query: 343 gctcgacgatggcaccggccgcctcgatttcatcagatgggtgaatgatgcttcagattc 402
|||||||| |||||||||||| |||| |||||||||||||||||||||||| |||| ||
Sbjct: 336 cctcgacgacggcaccggccgcatcgacttcatcagatgggtgaatgatgctgcagactc 395
Query: 403 ttttgaaactgctgctattcagaatggtatgtacattgcggtcattggaagcctcaaggg 462
| ||||||||||||||||||||| |||||||| |||| |||||||| |||||||||||
Sbjct: 396 tgctgaaactgctgctattcagaacggtatgtatgttgccgtcattgggagcctcaaggg 455
Query: 463 actgcaagagaggaagcgtgctactgctttctcaa-tcaggcctataaccgatttcaatg 521
| | || || | |||||||||||||||||| ||| |||| |||||||| |||| ||| |
Sbjct: 456 agttcaggacaagaagcgtgctactgcttttgcaagtcagacctataactgattacaacg 515
Query: 522 aggttacgctgcatttcattcagtgtgttcggatgcatatagagaaca 569
|||| || |||||||||||||||||||| ||||||||| ||||||||
Sbjct: 516 aggtcaccctgcatttcattcagtgtgtacggatgcatgcagagaaca 563
>gb|BI776636.2|BI776636 EBpi07_SQ001_D15_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ001_D15 5', mRNA sequence
Length = 526
Score = 165 bits (83), Expect = 4e-039
Identities = 176/207 (85%)
Strand = Plus / Plus
Query: 385 gaatgatgcttcagattcttttgaaactgctgctattcagaatggtatgtacattgcggt 444
|||||||||| |||| ||| ||||||||||||||||||||| |||||||| |||| ||
Sbjct: 3 gaatgatgctgcagactctgctgaaactgctgctattcagaacggtatgtatgttgccgt 62
Query: 445 cattggaagcctcaagggactgcaagagaggaagcgtgctactgctttctcaatcaggcc 504
|||||| |||||||||||| | || || | |||||||||||||||||| || |||| ||
Sbjct: 63 cattgggagcctcaagggagttcaggacaagaagcgtgctactgcttttgcagtcagacc 122
Query: 505 tataaccgatttcaatgaggttacgctgcatttcattcagtgtgttcggatgcatataga 564
|||||| |||| ||| ||||| || |||||||||||||||||||| ||||||||| |||
Sbjct: 123 tataactgattacaacgaggtcaccctgcatttcattcagtgtgtacggatgcatgcaga 182
Query: 565 gaacattgaattaaaggctggcagtcc 591
||||| ||| ||||| |||||||||
Sbjct: 183 gaacacaaaatcaaaggttggcagtcc 209
>gb|BM101281.2|BM101281 EBpi01_SQ003_G14_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_G14 5', mRNA sequence
Length = 496
Score = 44.1 bits (22), Expect = 0.011
Identities = 55/66 (83%)
Strand = Plus / Plus
Query: 293 gtggggatggtcaatgccaaggtggagcggacgaccgatgtgaccttcacgctcgacgat 352
||||||||||||| || ||||| |||||||| || ||||| ||||||| ||| |||||
Sbjct: 77 gtggggatggtcagtggcaaggcggagcggaacacagatgtttccttcacactcaacgat 136
Query: 353 ggcacc 358
| ||||
Sbjct: 137 gacacc 142
>gb|BU973701.1|BU973701 HB25L01r BC Hordeum vulgare subsp. vulgare cDNA clone HB25L01
5-PRIME, mRNA sequence
Length = 568
Score = 44.1 bits (22), Expect = 0.011
Identities = 55/66 (83%)
Strand = Plus / Plus
Query: 293 gtggggatggtcaatgccaaggtggagcggacgaccgatgtgaccttcacgctcgacgat 352
||||||||||||| || ||||| |||||||| || ||||| ||||||| ||| |||||
Sbjct: 196 gtggggatggtcagtggcaaggcggagcggaacacagatgtttccttcacactcaacgat 255
Query: 353 ggcacc 358
| ||||
Sbjct: 256 gacacc 261
>gb|CK124325.1|CK124325 BES1824108g01 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010G018 5-PRIME, mRNA sequence
Length = 618
Score = 42.1 bits (21), Expect = 0.044
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1166 ataattcagatgcaaaagcag 1186
|||||||||||||||||||||
Sbjct: 479 ataattcagatgcaaaagcag 499
>gb|BG343405.2|BG343405 HVSMEg0005K17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005K17f, mRNA sequence
Length = 929
Score = 40.1 bits (20), Expect = 0.18
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 171 gcggcgcgtccagcaccatg 190
||||||||||||||||||||
Sbjct: 27 gcggcgcgtccagcaccatg 8
>gb|CA019628.1|CA019628 HV12J16r HV Hordeum vulgare subsp. vulgare cDNA clone HV12J16
5-PRIME, mRNA sequence
Length = 413
Score = 40.1 bits (20), Expect = 0.18
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 171 gcggcgcgtccagcaccatg 190
||||||||||||||||||||
Sbjct: 102 gcggcgcgtccagcaccatg 83
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 128,079
Number of Sequences: 312970
Number of extensions: 128079
Number of successful extensions: 38247
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 38229
Number of HSP's gapped (non-prelim): 17
length of query: 1274
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1255
effective length of database: 169,188,109
effective search space: 212331076795
effective search space used: 212331076795
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)