BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716323.2.2
(764 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 359 9e-098
gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa... 250 6e-065
gb|CA028481.1|CA028481 HZ62C03r HZ Hordeum vulgare subsp. v... 123 9e-027
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 54 7e-006
gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. v... 46 0.002
gb|BM443397.1|BM443397 EBro02_SQ003_O24_R root, 3 week, hyd... 38 0.41
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 359 bits (181), Expect = 9e-098
Identities = 386/455 (84%)
Strand = Plus / Plus
Query: 109 tcgtcatgaaaggttatgtcggtgacgacgaggctactgcagcaacagtggattcagaag 168
||||||||||||||||||||||||||||| | || ||||| | ||||||||| |||||||
Sbjct: 17 tcgtcatgaaaggttatgtcggtgacgacaaagcaactgcggaaacagtggactcagaag 76
Query: 169 gctggttgaaaaccggtgacctatgctacttcaacgaggatgggttactctacattgttg 228
||||| ||||||| ||||| || ||||||||||| || ||||| || |||||||||||||
Sbjct: 77 gctggctgaaaactggtgatctttgctacttcaatgaagatggatttctctacattgttg 136
Query: 229 atcggttgaaagagttgataaagtacaagggatatcaggtacctcctgccgaactggagc 288
|||| ||||| ||||||||||||||||| ||||| ||||| ||||| || ||||||||||
Sbjct: 137 atcgtttgaaggagttgataaagtacaaaggataccaggtgcctccagctgaactggagc 196
Query: 289 atatcctgaattctcatcctgatatcatggacgcagcagttattccttatccagacgaag 348
|||| || | || |||||||| || || || |||||||| |||||||| ||||
Sbjct: 197 atattctacaatcacatcctgaaattgctgatgctgcagttatcggatatccagatgaag 256
Query: 349 acgccgggcaactcccaatggcttttatagtgaggaaaccagggtcaaaccttaccgagc 408
| | |||||||| |||||||| |||||||||||| ||||||| |||| |||||| | |
Sbjct: 257 atgttgggcaactgccaatggcctttatagtgaggcaaccaggttcaagccttactggga 316
Query: 409 aacaagtcatggatttcgtggctaaacaggtcgcaccctacaagaaagttcgtcgagtgg 468
||||||||||||||| |||||||||||| || || |||||||||||||| || |||||||
Sbjct: 317 aacaagtcatggattacgtggctaaacatgttgcgccctacaagaaagtccgccgagtgg 376
Query: 469 cctttgtttctgcaattcccaaatcgcctgctgggaagatcttgcggcgggagcttgtag 528
|||| || | ||||| |||||||| ||||| |||||||||||||| |||||||||||
Sbjct: 377 ccttcgtcgcggcaatacccaaatctcctgcggggaagatcttgcgacgggagcttgtgc 436
Query: 529 agcangccgtgtccatgggtacctncaagctttga 563
|||| ||| ||||||||||| ||| ||||||||||
Sbjct: 437 agcaagccatgtccatgggtgcctccaagctttga 471
>gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021B11f, mRNA sequence
Length = 491
Score = 250 bits (126), Expect = 6e-065
Identities = 330/398 (82%)
Strand = Plus / Plus
Query: 6 gtcgggaagctggcatcgcacttggaggcaaagatcgtcgatccctccaccggcgaggtg 65
||||| |||||||| ||||||||| | || ||||| |||||||| ||||||||||||| |
Sbjct: 85 gtcggcaagctggcgtcgcacttgcaagcgaagatagtcgatccgtccaccggcgaggcg 144
Query: 66 cttgggccagggcagcgcggcgagctctgggtccgaggaccagtcgtcatgaaaggttat 125
|| |||| || |||||||||||||||| | || || ||| |||||||||||||||||
Sbjct: 145 ctctggccgggccagcgcggcgagctcttgatcaagggcccactcgtcatgaaaggttat 204
Query: 126 gtcggtgacgacgaggctactgcagcaacagtggattcagaaggctggttgaaaaccggt 185
|| ||||||||| | || ||||| | ||||||||| |||||||||||| | ||||| |||
Sbjct: 205 gttggtgacgacaaagcaactgcggaaacagtggactcagaaggctggcttaaaactggt 264
Query: 186 gacctatgctacttcaacgaggatgggttactctacattgttgatcggttgaaagagttg 245
|| || ||||||||||| || ||||| || ||||||||||||||||| ||||| ||||||
Sbjct: 265 gatctttgctacttcaatgaagatggatttctctacattgttgatcgtttgaaggagttg 324
Query: 246 ataaagtacaagggatatcaggtacctcctgccgaactggagcatatcctgaattctcat 305
||||||||||| ||||| |||| ||||| || |||||||||||||| || | || |||
Sbjct: 325 ataaagtacaaaggataccaggggcctccagctgaactggagcatattctacaatcacat 384
Query: 306 cctgatatcatggacgcagcagttattccttatccagacgaagacgccgggcaactccca 365
||||| || || || |||||||| |||||||| ||||| | |||||||| |||
Sbjct: 385 cctgaaattgctgatgctgcagttataggatatccagatgaagatgttgggcaactgcca 444
Query: 366 atggcttttatagtgaggaaaccagggtcaaaccttac 403
||||| |||||||||||| ||||||| |||| ||||||
Sbjct: 445 atggcctttatagtgaggcaaccaggttcaagccttac 482
>gb|CA028481.1|CA028481 HZ62C03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62C03
5-PRIME, mRNA sequence
Length = 495
Score = 123 bits (62), Expect = 9e-027
Identities = 117/136 (86%)
Strand = Plus / Plus
Query: 428 ggctaaacaggtcgcaccctacaagaaagttcgtcgagtggcctttgtttctgcaattcc 487
||||||||| || || |||||||||||||| || ||||||||||| || | ||||| ||
Sbjct: 10 ggctaaacatgttgcgccctacaagaaagtccgccgagtggccttcgtcgcggcaatacc 69
Query: 488 caaatcgcctgctgggaagatcttgcggcgggagcttgtagagcangccgtgtccatggg 547
|||||| ||||| |||||||||||||| ||||||||||| |||| ||| ||||||||||
Sbjct: 70 caaatctcctgcggggaagatcttgcgacgggagcttgtgcagcaagccatgtccatggg 129
Query: 548 tacctncaagctttga 563
| ||| ||||||||||
Sbjct: 130 tgcctccaagctttga 145
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 54.0 bits (27), Expect = 7e-006
Identities = 78/95 (82%)
Strand = Plus / Plus
Query: 180 accggtgacctatgctacttcaacgaggatgggttactctacattgttgatcggttgaaa 239
|||||||||||||||||| | |||||||||||| |||| | | || || || |||||
Sbjct: 255 accggtgacctatgctacatagacgaggatgggtatctcttcgtggtggaccgtctgaaa 314
Query: 240 gagttgataaagtacaagggatatcaggtacctcc 274
|||||||| || ||||| || |||||||| |||||
Sbjct: 315 gagttgatcaaatacaaaggctatcaggtgcctcc 349
>gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
5-PRIME, mRNA sequence
Length = 581
Score = 46.1 bits (23), Expect = 0.002
Identities = 71/87 (81%)
Strand = Plus / Plus
Query: 180 accggtgacctatgctacttcaacgaggatgggttactctacattgttgatcggttgaaa 239
|||||||||||||||||| | |||||||||||| |||| | | || || || |||||
Sbjct: 385 accggtgacctatgctacatagacgaggatgggtatctcttcgtggtggaccgtctgaaa 444
Query: 240 gagttgataaagtacaagggatatcag 266
|||||||| || ||||| || ||||||
Sbjct: 445 gagttgatcaaatacaaaggctatcag 471
>gb|BM443397.1|BM443397 EBro02_SQ003_O24_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_O24 5', mRNA sequence
Length = 364
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 486 cccaaatcgcctgctgggaagatcttg 512
||||||||||| || ||||||||||||
Sbjct: 98 cccaaatcgccggccgggaagatcttg 124
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 66,108
Number of Sequences: 312970
Number of extensions: 66108
Number of successful extensions: 18396
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18382
Number of HSP's gapped (non-prelim): 11
length of query: 764
length of database: 175,134,539
effective HSP length: 19
effective length of query: 745
effective length of database: 169,188,109
effective search space: 126045141205
effective search space used: 126045141205
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)