BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716323.2.1
(724 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 317 3e-085
gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa... 151 4e-035
gb|CA028481.1|CA028481 HZ62C03r HZ Hordeum vulgare subsp. v... 127 5e-028
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 40 0.098
gb|BM443397.1|BM443397 EBro02_SQ003_O24_R root, 3 week, hyd... 38 0.39
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 317 bits (160), Expect = 3e-085
Identities = 372/445 (83%)
Strand = Plus / Plus
Query: 89 aggttatgtcggtgacgacgaggctactgcagcaacagtggattcagaaggctggttgaa 148
||||||||||||||||||| | || ||||| | ||||||||| |||||||||||| ||||
Sbjct: 27 aggttatgtcggtgacgacaaagcaactgcggaaacagtggactcagaaggctggctgaa 86
Query: 149 aaccggtnnnnnnngctacttcaacgaggatgggttactctacattgttgatcggttgaa 208
||| ||| |||||||||| || ||||| || ||||||||||||||||| |||||
Sbjct: 87 aactggtgatctttgctacttcaatgaagatggatttctctacattgttgatcgtttgaa 146
Query: 209 agagttgataaagtacaagggatatcaggtacctcctgccgaactggagcatatcctgaa 268
||||||||||||||||| ||||| ||||| ||||| || |||||||||||||| || |
Sbjct: 147 ggagttgataaagtacaaaggataccaggtgcctccagctgaactggagcatattctaca 206
Query: 269 ttctcatcctgatatcatggacgcagcagttattccttatccagacgaagacgccgggca 328
|| |||||||| || || || |||||||| |||||||| ||||| | |||||
Sbjct: 207 atcacatcctgaaattgctgatgctgcagttatcggatatccagatgaagatgttgggca 266
Query: 329 actcccaatggcttttatagtgaggaaaccagggtcaaaccttaccgagcaacaagtcat 388
||| |||||||| |||||||||||| ||||||| |||| |||||| | | ||||||||||
Sbjct: 267 actgccaatggcctttatagtgaggcaaccaggttcaagccttactgggaaacaagtcat 326
Query: 389 ggatttcgtggctaaacaggtcgcaccctacaagaaagttcgtcgagtggcctttgtttc 448
||||| |||||||||||| || || |||||||||||||| || ||||||||||| || |
Sbjct: 327 ggattacgtggctaaacatgttgcgccctacaagaaagtccgccgagtggccttcgtcgc 386
Query: 449 tgcaatccccaaatcgcctgctgggaagatcttgcggcgggagcttgtagagcaggccgt 508
||||| |||||||| ||||| |||||||||||||| ||||||||||| |||| ||| |
Sbjct: 387 ggcaatacccaaatctcctgcggggaagatcttgcgacgggagcttgtgcagcaagccat 446
Query: 509 gtccatgggtacctccaagctttga 533
|||||||||| ||||||||||||||
Sbjct: 447 gtccatgggtgcctccaagctttga 471
>gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021B11f, mRNA sequence
Length = 491
Score = 151 bits (76), Expect = 4e-035
Identities = 231/285 (81%)
Strand = Plus / Plus
Query: 89 aggttatgtcggtgacgacgaggctactgcagcaacagtggattcagaaggctggttgaa 148
||||||||| ||||||||| | || ||||| | ||||||||| |||||||||||| | ||
Sbjct: 198 aggttatgttggtgacgacaaagcaactgcggaaacagtggactcagaaggctggcttaa 257
Query: 149 aaccggtnnnnnnngctacttcaacgaggatgggttactctacattgttgatcggttgaa 208
||| ||| |||||||||| || ||||| || ||||||||||||||||| |||||
Sbjct: 258 aactggtgatctttgctacttcaatgaagatggatttctctacattgttgatcgtttgaa 317
Query: 209 agagttgataaagtacaagggatatcaggtacctcctgccgaactggagcatatcctgaa 268
||||||||||||||||| ||||| |||| ||||| || |||||||||||||| || |
Sbjct: 318 ggagttgataaagtacaaaggataccaggggcctccagctgaactggagcatattctaca 377
Query: 269 ttctcatcctgatatcatggacgcagcagttattccttatccagacgaagacgccgggca 328
|| |||||||| || || || |||||||| |||||||| ||||| | |||||
Sbjct: 378 atcacatcctgaaattgctgatgctgcagttataggatatccagatgaagatgttgggca 437
Query: 329 actcccaatggcttttatagtgaggaaaccagggtcaaaccttac 373
||| |||||||| |||||||||||| ||||||| |||| ||||||
Sbjct: 438 actgccaatggcctttatagtgaggcaaccaggttcaagccttac 482
>gb|CA028481.1|CA028481 HZ62C03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62C03
5-PRIME, mRNA sequence
Length = 495
Score = 127 bits (64), Expect = 5e-028
Identities = 118/136 (86%)
Strand = Plus / Plus
Query: 398 ggctaaacaggtcgcaccctacaagaaagttcgtcgagtggcctttgtttctgcaatccc 457
||||||||| || || |||||||||||||| || ||||||||||| || | ||||| ||
Sbjct: 10 ggctaaacatgttgcgccctacaagaaagtccgccgagtggccttcgtcgcggcaatacc 69
Query: 458 caaatcgcctgctgggaagatcttgcggcgggagcttgtagagcaggccgtgtccatggg 517
|||||| ||||| |||||||||||||| ||||||||||| |||| ||| ||||||||||
Sbjct: 70 caaatctcctgcggggaagatcttgcgacgggagcttgtgcagcaagccatgtccatggg 129
Query: 518 tacctccaagctttga 533
| ||||||||||||||
Sbjct: 130 tgcctccaagctttga 145
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 40.1 bits (20), Expect = 0.098
Identities = 35/40 (87%)
Strand = Plus / Plus
Query: 205 tgaaagagttgataaagtacaagggatatcaggtacctcc 244
||||||||||||| || ||||| || |||||||| |||||
Sbjct: 310 tgaaagagttgatcaaatacaaaggctatcaggtgcctcc 349
>gb|BM443397.1|BM443397 EBro02_SQ003_O24_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_O24 5', mRNA sequence
Length = 364
Score = 38.2 bits (19), Expect = 0.39
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 456 cccaaatcgcctgctgggaagatcttg 482
||||||||||| || ||||||||||||
Sbjct: 98 cccaaatcgccggccgggaagatcttg 124
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 57,297
Number of Sequences: 312970
Number of extensions: 57297
Number of successful extensions: 16105
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16094
Number of HSP's gapped (non-prelim): 9
length of query: 724
length of database: 175,134,539
effective HSP length: 19
effective length of query: 705
effective length of database: 169,188,109
effective search space: 119277616845
effective search space used: 119277616845
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)