BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCL28h07.yg.2.1
         (277 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF118559.1|AF118559  Avena fatua puroindoline precursor, ...    46   2e-005
gb|CN815207.1|CN815207  HRO4507_A11_B21ZS5 Lib AA071E1X Aven...    34   0.061
gb|CN820684.1|CN820684  HRO4416_C12_F24ZS5 Lib AA069E1X Aven...    32   0.24 
gb|CN821249.1|CN821249  HRO4423_B10_D19ZS5 Lib AA069E1X Aven...    32   0.24 
>gb|AF118559.1|AF118559 Avena fatua puroindoline precursor, mRNA, complete cds
          Length = 571

 Score = 46.1 bits (23), Expect = 2e-005
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                  
Query: 254 tacctgcccgggcggccgctcga 276
           |||||||||||||||||||||||
Sbjct: 23  tacctgcccgggcggccgctcga 1
>gb|CN815207.1|CN815207 HRO4507_A11_B21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4507_A11_B21, mRNA sequence
          Length = 611

 Score = 34.2 bits (17), Expect = 0.061
 Identities = 17/17 (100%)
 Strand = Plus / Minus

                            
Query: 19  atgactgcccaaatatt 35
           |||||||||||||||||
Sbjct: 478 atgactgcccaaatatt 462
>gb|CN820684.1|CN820684 HRO4416_C12_F24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4416_C12_F24, mRNA
           sequence
          Length = 437

 Score = 32.2 bits (16), Expect = 0.24
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                               
Query: 39  tacacaataagcaaaatcat 58
           ||||||||||||||| ||||
Sbjct: 378 tacacaataagcaaattcat 359
>gb|CN821249.1|CN821249 HRO4423_B10_D19ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4423_B10_D19, mRNA
           sequence
          Length = 568

 Score = 32.2 bits (16), Expect = 0.24
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 157 attcagctcctccatg 172
           ||||||||||||||||
Sbjct: 227 attcagctcctccatg 212
  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 700
Number of Sequences: 8143
Number of extensions: 700
Number of successful extensions: 194
Number of sequences better than  0.5: 4
Number of HSP's better than  0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 190
Number of HSP's gapped (non-prelim): 4
length of query: 277
length of database: 4,702,463
effective HSP length: 15
effective length of query: 262
effective length of database: 4,580,318
effective search space: 1200043316
effective search space used: 1200043316
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)