BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAF25f04.yg.3.3
         (1599 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN818871.1|CN818871  HRO4456_A03_A06ZS5 Lib AA070E1X Aven...   151   2e-036
gb|BE439322.1|BE439322  CDO1407_WHE05G08F ITEC CDO Oat cDNA ...    34   0.37 

>gb|CN818871.1|CN818871 HRO4456_A03_A06ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
            Avena sativa cDNA clone HRO4456_A03_A06, mRNA sequence
          Length = 609

 Score =  151 bits (76), Expect = 2e-036
 Identities = 142/164 (86%)
 Strand = Plus / Plus

                                                                        
Query: 1037 gagatttcctggtgcagtcgaaggagttctcagctcaggataacaaggatctatacgcgg 1096
            |||| ||||||||||| ||||||||||| || |  ||||||||||||||||| || ||||
Sbjct: 11   gagacttcctggtgcaatcgaaggagttttcggtgcaggataacaaggatctgtatgcgg 70

                                                                        
Query: 1097 aagaggctgctgcccaaagagaaagggagcgccaacgaatgcttgccattccggggctga 1156
            ||||||||||||  |||||||||| ||| || ||||| ||| |  ||||||| ||||| |
Sbjct: 71   aagaggctgctgtacaaagagaaaaggaacggcaacggatgatgtccattcctgggctca 130

                                                        
Query: 1157 ttgcccctagcgaattgcaagacgagatggtagattcgtagtgt 1200
            ||||||| || |||||||||||||||||||||||||| ||||||
Sbjct: 131  ttgccccgagtgaattgcaagacgagatggtagattcatagtgt 174




 Score = 67.9 bits (34), Expect = 3e-011
 Identities = 49/54 (90%)
 Strand = Plus / Plus

                                                                  
Query: 1286 tcttccagggtggtgaaaggtggctgctgtaattttatttttggttacctccac 1339
            ||||||| |||||||||||||| |||||||||||||||| | |||| |||||||
Sbjct: 249  tcttccatggtggtgaaaggtgcctgctgtaattttattctcggttgcctccac 302




 Score = 36.2 bits (18), Expect = 0.093
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                  
Query: 1552 gatccaggctgataggaacctt 1573
            ||||||| ||||||||||||||
Sbjct: 548  gatccagactgataggaacctt 569



>gb|BE439322.1|BE439322 CDO1407_WHE05G08F ITEC CDO Oat cDNA Library Avena sativa cDNA clone
           CDO1407_WHE05G08, mRNA sequence
          Length = 399

 Score = 34.2 bits (17), Expect = 0.37
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 687 gaaaaaattccaggctt 703
           |||||||||||||||||
Sbjct: 132 gaaaaaattccaggctt 148



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 4317
Number of Sequences: 8143
Number of extensions: 4317
Number of successful extensions: 1094
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1090
Number of HSP's gapped (non-prelim): 4
length of query: 1599
length of database: 4,702,463
effective HSP length: 17
effective length of query: 1582
effective length of database: 4,564,032
effective search space: 7220298624
effective search space used: 7220298624
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)