BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3204272.2.1
         (739 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN815151.1|CN815151  HRO4506_D10_G20ZS5 Lib AA071E1X Aven...   135   6e-032
gb|CN818949.1|CN818949  HRO4474_G09_N18ZS5 Lib AA070E1X Aven...   119   4e-027
>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4506_D10_G20, mRNA sequence
          Length = 599

 Score =  135 bits (68), Expect = 6e-032
 Identities = 184/220 (83%), Gaps = 2/220 (0%)
 Strand = Plus / Minus

                                                                       
Query: 192 tcacatctccgctacaagcttggactgagccagggcgagatcgaacgagctgaaggagcg 251
           |||||||||||| |||||||  ||||||||||| || || |||||||||  ||||||| |
Sbjct: 333 tcacatctccgcgacaagctgtgactgagccagcgcaaggtcgaacgaggagaaggagtg 274

                                                                       
Query: 252 aggagccagcgtgacctgcatctgctctgcagcattgctcagatcgctctttaccggcac 311
           || ||  || ||||||||||||||||| ||||| |||| ||| | |||  | || |||||
Sbjct: 273 agcagggagtgtgacctgcatctgctccgcagcgttgcgcagctggcttgtcacaggcac 214

                                                                       
Query: 312 aaccttggttgggttgctgaaggagttttcatccatcacatctgct-gatgtgagaacag 370
           ||||||| ||||||| |||||||| |||||||||||||||| |||| |||||||||||||
Sbjct: 213 aaccttgtttgggttactgaaggaattttcatccatcacat-tgctggatgtgagaacag 155

                                                   
Query: 371 tggcggttgatcccagagcgttgatactggcctggagccc 410
           | ||| | || || |||| |||||| || ||||| |||||
Sbjct: 154 tagcgttcgaccctagagtgttgatgctagcctgaagccc 115
>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
          Length = 495

 Score =  119 bits (60), Expect = 4e-027
 Identities = 182/220 (82%), Gaps = 2/220 (0%)
 Strand = Plus / Minus

                                                                       
Query: 192 tcacatctccgctacaagcttggactgagccagggcgagatcgaacgagctgaaggagcg 251
           |||||||||||| |||||||  ||||||||||| || || |||||||||  ||||||| |
Sbjct: 299 tcacatctccgcgacaagctgtgactgagccagcgcaaggtcgaacgaggagaaggagtg 240

                                                                       
Query: 252 aggagccagcgtgacctgcatctgctctgcagcattgctcagatcgctctttaccggcac 311
           || ||  || ||||||||||||||||| ||||| |||| ||| | |||  | || |||||
Sbjct: 239 agcagggagtgtgacctgcatctgctccgcagcgttgcgcagctggcttgtcacaggcac 180

                                                                       
Query: 312 aaccttggttgggttgctgaaggagttttcatccatcacatctgct-gatgtgagaacag 370
           ||||||| | ||||| |||||||| |||||||||||||||| |||| || || |||||||
Sbjct: 179 aaccttgttcgggttactgaaggaattttcatccatcacat-tgctggacgtcagaacag 121

                                                   
Query: 371 tggcggttgatcccagagcgttgatactggcctggagccc 410
           | ||| | || ||||| |||||||| || ||||| |||||
Sbjct: 120 tagcgttcgaacccagcgcgttgatgctagcctgaagccc 81
  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1721
Number of Sequences: 8143
Number of extensions: 1721
Number of successful extensions: 481
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 477
Number of HSP's gapped (non-prelim): 3
length of query: 739
length of database: 4,702,463
effective HSP length: 16
effective length of query: 723
effective length of database: 4,572,175
effective search space: 3305682525
effective search space used: 3305682525
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)