BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3203838.2.1
         (534 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF118559.1|AF118559  Avena fatua puroindoline precursor, ...    46   3e-005
gb|CN814864.1|CN814864  HRO4503_C11_F21ZS5 Lib AA071E1X Aven...    36   0.031
gb|CN820854.1|CN820854  HRO4420_G01_N02ZS5 Lib AA069E1X Aven...    36   0.031
gb|CN815761.1|CN815761  HRO4510_C01_E02ZS5 Lib AA071E1X Aven...    34   0.12 
gb|CN815915.1|CN815915  HRO4511_H05_P09ZS5 Lib AA071E1X Aven...    34   0.12 
gb|AY277385.1|  Avena sativa 1,3-beta glucanase (Oglc13) gen...    32   0.48 
gb|AF155932.1|  Avena sativa 1,3-beta glucanase (Oglc13) mRN...    32   0.48 
>gb|AF118559.1|AF118559 Avena fatua puroindoline precursor, mRNA, complete cds
          Length = 571

 Score = 46.1 bits (23), Expect = 3e-005
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                  
Query: 511 tacctgcccgggcggccgctcga 533
           |||||||||||||||||||||||
Sbjct: 23  tacctgcccgggcggccgctcga 1
>gb|CN814864.1|CN814864 HRO4503_C11_F21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4503_C11_F21, mRNA sequence
          Length = 547

 Score = 36.2 bits (18), Expect = 0.031
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 233 tgccgatgtcgatgacga 250
           ||||||||||||||||||
Sbjct: 363 tgccgatgtcgatgacga 380
>gb|CN820854.1|CN820854 HRO4420_G01_N02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4420_G01_N02, mRNA
           sequence
          Length = 379

 Score = 36.2 bits (18), Expect = 0.031
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 233 tgccgatgtcgatgacga 250
           ||||||||||||||||||
Sbjct: 341 tgccgatgtcgatgacga 358
>gb|CN815761.1|CN815761 HRO4510_C01_E02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4510_C01_E02, mRNA sequence
          Length = 628

 Score = 34.2 bits (17), Expect = 0.12
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 432 ggcagagatctgcagag 448
           |||||||||||||||||
Sbjct: 200 ggcagagatctgcagag 216
>gb|CN815915.1|CN815915 HRO4511_H05_P09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4511_H05_P09, mRNA sequence
          Length = 842

 Score = 34.2 bits (17), Expect = 0.12
 Identities = 23/25 (92%)
 Strand = Plus / Plus

                                    
Query: 351 gaagtccatcatctcctgcgtgtcc 375
           ||||| |||||||||||| ||||||
Sbjct: 388 gaagttcatcatctcctgggtgtcc 412

 Score = 32.2 bits (16), Expect = 0.48
 Identities = 25/28 (89%)
 Strand = Plus / Plus

                                       
Query: 236 cgatgtcgatgacgaagcggtacctgac 263
           |||||||||| || || |||||||||||
Sbjct: 273 cgatgtcgattacaaaccggtacctgac 300
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
          Length = 3281

 Score = 32.2 bits (16), Expect = 0.48
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                                
Query: 231  gttgccgatgtcgatgacga 250
            |||||||||||| |||||||
Sbjct: 2413 gttgccgatgtccatgacga 2394
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
          Length = 1045

 Score = 32.2 bits (16), Expect = 0.48
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                               
Query: 231 gttgccgatgtcgatgacga 250
           |||||||||||| |||||||
Sbjct: 168 gttgccgatgtccatgacga 149
  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1073
Number of Sequences: 8143
Number of extensions: 1073
Number of successful extensions: 272
Number of sequences better than  0.5: 7
Number of HSP's better than  0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 264
Number of HSP's gapped (non-prelim): 8
length of query: 534
length of database: 4,702,463
effective HSP length: 16
effective length of query: 518
effective length of database: 4,572,175
effective search space: 2368386650
effective search space used: 2368386650
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)