BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071761.2.1
(638 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN817366.1|CN817366 HRO4465_D03_H05ZS5 Lib AA070E1X Aven... 373 e-103
gb|CN816052.1|CN816052 HRO4516_D03_H06ZS5 Lib AA071E1X Aven... 216 2e-056
gb|CN821589.1|CN821589 HRO4408_A07_B14Z Lib AA069E1X Avena ... 34 0.14
>gb|CN817366.1|CN817366 HRO4465_D03_H05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4465_D03_H05, mRNA sequence
Length = 633
Score = 373 bits (188), Expect = e-103
Identities = 295/328 (89%), Gaps = 2/328 (0%)
Strand = Plus / Plus
Query: 1 cttcatcgtcgacaggctcaaggagatcatcaagtacaagggcttccaggtgccgccggc 60
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct: 41 cttcatcgtcgacaggctcaaggagatcatcaagtacaagggcttccaggtggcgccggc 100
Query: 61 ggagctggaggcgctcctcatcacgcacccggagatcaaggacgccgccgtcgtatcaat 120
|||||| ||||| |||||||||||||||||||| ||||||||||||||||||||| | |
Sbjct: 101 ggagctcgaggccctcctcatcacgcacccggacatcaaggacgccgccgtcgtaggagt 160
Query: 121 gaacgatgacct-tgctggtgaaatcccggtcgccttcatcgtgcggaccgaaggttctc 179
||| ||||| || ||| || ||| | ||||||||||||||| ||||| |||||| |||
Sbjct: 161 gaaggatgatctctgc-ggcgaagtgccggtcgccttcatcaagcggatcgaagggtctg 219
Query: 180 aagtcaccgaggatgagatcaagcaattcgtcgccaaggaggtggttttctacaagaaga 239
| |||||||| | |||||||||||||||||| |||||||||| ||||||||||||| ||
Sbjct: 220 agatcaccgagaacgagatcaagcaattcgtctccaaggaggttgttttctacaagagga 279
Query: 240 tccacaaggtcttcttcaccgaatccatccccaagaacccgtcgggcaagatcctgagga 299
|| ||||||||| ||||||||| ||||| ||||||||||| || ||||||||||| ||||
Sbjct: 280 tcaacaaggtctacttcaccgactccattcccaagaacccttccggcaagatcctaagga 339
Query: 300 aggacttgagagccaggctcgccgccgg 327
||||||||||||||||||||||||||||
Sbjct: 340 aggacttgagagccaggctcgccgccgg 367
>gb|CN816052.1|CN816052 HRO4516_D03_H06ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_D03_H06, mRNA sequence
Length = 547
Score = 216 bits (109), Expect = 2e-056
Identities = 198/225 (88%), Gaps = 2/225 (0%)
Strand = Plus / Plus
Query: 104 gccgccgtcgtatcaatgaacgatgacct-tgctggtgaaatcccggtcgccttcatcgt 162
||||||||||||| | |||| ||||| || ||| || ||| |||||||||||||||||
Sbjct: 11 gccgccgtcgtatgagtgaaggatgatctctgc-ggcgaagtcccggtcgccttcatcaa 69
Query: 163 gcggaccgaaggttctcaagtcaccgaggatgagatcaagcaattcgtcgccaaggaggt 222
||||| |||||| ||| | |||||||| | |||||||||||||||||| ||||||||||
Sbjct: 70 gcggatcgaaggatctgagatcaccgagaacgagatcaagcaattcgtctccaaggaggt 129
Query: 223 ggttttctacaagaagatccacaaggtcttcttcaccgaatccatccccaagaacccgtc 282
||||||||||||| |||| ||||||||| ||||||||| ||||| ||||||||||| ||
Sbjct: 130 tgttttctacaagaggatcaacaaggtctacttcaccgactccattcccaagaacccttc 189
Query: 283 gggcaagatcctgaggaaggacttgagagccaggctcgccgccgg 327
||||||||||| ||||||||||||||||||||||||||||||||
Sbjct: 190 cggcaagatcctaaggaaggacttgagagccaggctcgccgccgg 234
>gb|CN821589.1|CN821589 HRO4408_A07_B14Z Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_A07_B14, mRNA
sequence
Length = 537
Score = 34.2 bits (17), Expect = 0.14
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 124 cgatgaccttgctggtg 140
|||||||||||||||||
Sbjct: 74 cgatgaccttgctggtg 58
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1919
Number of Sequences: 8143
Number of extensions: 1919
Number of successful extensions: 487
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 478
Number of HSP's gapped (non-prelim): 8
length of query: 638
length of database: 4,702,463
effective HSP length: 16
effective length of query: 622
effective length of database: 4,572,175
effective search space: 2843892850
effective search space used: 2843892850
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)