BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2750422.2.1
         (1599 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN821419.1|CN821419  HRO4425_B04_C07ZS5 Lib AA069E1X Aven...   139   9e-033
gb|AF118559.1|AF118559  Avena fatua puroindoline precursor, ...    46   1e-004
gb|CN818618.1|CN818618  HRO4467_C08_E15ZS5 Lib AA070E1X Aven...    34   0.37 
>gb|CN821419.1|CN821419 HRO4425_B04_C07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4425_B04_C07, mRNA
           sequence
          Length = 744

 Score =  139 bits (70), Expect = 9e-033
 Identities = 145/170 (85%)
 Strand = Plus / Minus

                                                                       
Query: 606 ttgaactgcaccaggatcatggtcatgttgtcgcacccgtctccagcgatcgttgatgga 665
           ||||||||||| |||||||| ||||||||||| || || |||||| | |  |||||||||
Sbjct: 180 ttgaactgcactaggatcattgtcatgttgtcacatccttctccacctaatgttgatgga 121

                                                                       
Query: 666 gccaggcatctgtccagcactctctcgcacacggcagacaggctgctctccgtgtttata 725
           ||||||||||| || |||||  | || ||||| ||||| ||||||||||| |||||||| 
Sbjct: 120 gccaggcatctatcgagcaccttttcacacaccgcagaaaggctgctctctgtgtttatg 61

                                                             
Query: 726 cgctcgtggataaaatccaccagctgttggcttgacatgcagtcccaaat 775
            |||| ||||  ||||| |||||||||||||| |||||||||||||||||
Sbjct: 60  tgctcatggacgaaatcgaccagctgttggctagacatgcagtcccaaat 11
>gb|AF118559.1|AF118559 Avena fatua puroindoline precursor, mRNA, complete cds
          Length = 571

 Score = 46.1 bits (23), Expect = 1e-004
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                   
Query: 1576 tacctgcccgggcggccgctcga 1598
            |||||||||||||||||||||||
Sbjct: 23   tacctgcccgggcggccgctcga 1
>gb|CN818618.1|CN818618 HRO4467_C08_E15ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4467_C08_E15, mRNA sequence
          Length = 481

 Score = 34.2 bits (17), Expect = 0.37
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 873 aatttgttctgtttcaa 889
           |||||||||||||||||
Sbjct: 450 aatttgttctgtttcaa 466
  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 4212
Number of Sequences: 8143
Number of extensions: 4212
Number of successful extensions: 1002
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 998
Number of HSP's gapped (non-prelim): 4
length of query: 1599
length of database: 4,702,463
effective HSP length: 17
effective length of query: 1582
effective length of database: 4,564,032
effective search space: 7220298624
effective search space used: 7220298624
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)