BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2169937.2.1
(891 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRN... 100 4e-021
gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gen... 78 2e-014
gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Aven... 76 6e-014
emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase 64 2e-010
gb|CN815155.1|CN815155 HRO4506_E03_I06ZS5 Lib AA071E1X Aven... 42 8e-004
gb|CN821383.1|CN821383 HRO4424_F11_L22ZS5 Lib AA069E1X Aven... 34 0.20
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
Length = 1045
Score = 99.6 bits (50), Expect = 4e-021
Identities = 273/345 (79%), Gaps = 2/345 (0%)
Strand = Plus / Plus
Query: 283 tacccttacttcgcgtaccgggacaacccgcgcgacatc-agctggg-tacgccacgttc 340
||||| |||||| ||||||| ||||||||||| |||||| |||| ||||| ||||||
Sbjct: 499 tacccctacttctcgtaccgcgacaacccgcgtgacatccagctcaactacgcgacgttc 558
Query: 341 cagccgggcaccaccgtcagggacaacggcaacggcctcaactacaacaacctgttcgac 400
| |||||||||||| || |||||| | | ||| || | ||||| ||||||||||||
Sbjct: 559 cggccgggcaccacggtgcgggacagcaagagcgggctgacctacacgaacctgttcgac 618
Query: 401 gccatggtggacgccgtcgtcgccgcgctcgagaaggccggcgcgcccaacgtcagggtc 460
|| ||||||||||| || ||| ||||| |||||||| || ||| | | ||| | |||
Sbjct: 619 gcgatggtggacgcggtgcacgcggcgctggagaaggcgggggcggcgagcgtgaaggtg 678
Query: 461 gtcgtgtccgagancggctggccctcggccggcggcttcggggccagcgtcgacaacgcg 520
|| ||||| |||| ||| ||||| || ||||| ||| || ||||| |||||||||
Sbjct: 679 gtggtgtcggagagcgggtggccgaaggagggcgggaccggcgcgagcgtggacaacgcg 738
Query: 521 cggaaatacaaccagggactcatcgaccacgtcggccgcggcacgcccaagaggaccggc 580
|| ||||||||||| || |||||||||||||| | ||||| ||||||| | ||||
Sbjct: 739 agggcgtacaaccaggggctgatcgaccacgtcggtgggggcacccccaagaagcgcggc 798
Query: 581 ccgctggagaccttcgtcttcgccatgttcaacgagaaccagaag 625
|||||||||| | ||| |||||||||||| ||||||||||||||
Sbjct: 799 gcgctggagacgtacgtgttcgccatgttcgacgagaaccagaag 843
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
Length = 3281
Score = 77.8 bits (39), Expect = 2e-014
Identities = 81/95 (85%)
Strand = Plus / Plus
Query: 527 tacaaccagggactcatcgaccacgtcggccgcggcacgcccaagaggaccggcccgctg 586
||||||||||| || ||||||||||||||| | ||||| ||||||| | |||| |||||
Sbjct: 2990 tacaaccaggggctgatcgaccacgtcggcgggggcacccccaagaagcgcggcgcgctg 3049
Query: 587 gagaccttcgtcttcgccatgttcaacgagaacca 621
||||| | ||| ||||| |||||| ||||||||||
Sbjct: 3050 gagacgtacgtgttcgcgatgttcgacgagaacca 3084
>gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G03_M06, mRNA
sequence
Length = 508
Score = 75.8 bits (38), Expect = 6e-014
Identities = 142/177 (80%)
Strand = Plus / Plus
Query: 449 aacgtcagggtcgtcgtgtccgagancggctggccctcggccggcggcttcggggccagc 508
||||||| | ||||||||||||||| ||| |||||||||||||||||| || ||||||
Sbjct: 105 aacgtcaagatcgtcgtgtccgagagcgggtggccctcggccggcggcaccgccgccagc 164
Query: 509 gtcgacaacgcgcggaaatacaaccagggactcatcgaccacgtcggccgcggcacgccc 568
| || ||||||| || ||||||||| |||||| |||| ||||| || ||||||
Sbjct: 165 gccgctaacgcgcagacctacaaccagaacctcatcaaccatgtcgggaaggggacgccc 224
Query: 569 aagaggaccggcccgctggagaccttcgtcttcgccatgttcaacgagaaccagaag 625
|||||| |||| | | ||||| | | | ||||||||||||||||||||| |||||
Sbjct: 225 aagaggcccggagccatcgagacatacatattcgccatgttcaacgagaacaagaag 281
>emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase
Length = 1448
Score = 63.9 bits (32), Expect = 2e-010
Identities = 38/40 (95%)
Strand = Plus / Plus
Query: 587 gagaccttcgtcttcgccatgttcaacgagaaccagaagg 626
||||||| ||| ||||||||||||||||||||||||||||
Sbjct: 922 gagacctacgtgttcgccatgttcaacgagaaccagaagg 961
Score = 38.2 bits (19), Expect = 0.013
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 389 aacctgttcgacgccatggtggacgcc 415
|||||||||||| ||| ||||||||||
Sbjct: 724 aacctgttcgacaccacggtggacgcc 750
Score = 36.2 bits (18), Expect = 0.052
Identities = 29/33 (87%)
Strand = Plus / Plus
Query: 464 gtgtccgagancggctggccctcggccggcggc 496
|||||||||| || ||||| ||||||||||||
Sbjct: 799 gtgtccgagagtgggtggccgtcggccggcggc 831
>gb|CN815155.1|CN815155 HRO4506_E03_I06ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_E03_I06, mRNA sequence
Length = 333
Score = 42.1 bits (21), Expect = 8e-004
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 599 ttcgccatgttcaacgagaac 619
|||||||||||||||||||||
Sbjct: 52 ttcgccatgttcaacgagaac 72
>gb|CN821383.1|CN821383 HRO4424_F11_L22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4424_F11_L22, mRNA
sequence
Length = 518
Score = 34.2 bits (17), Expect = 0.20
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 689 atccggttcaactaatt 705
|||||||||||||||||
Sbjct: 282 atccggttcaactaatt 266
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1405
Number of Sequences: 8143
Number of extensions: 1405
Number of successful extensions: 407
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 388
Number of HSP's gapped (non-prelim): 19
length of query: 891
length of database: 4,702,463
effective HSP length: 16
effective length of query: 875
effective length of database: 4,572,175
effective search space: 4000653125
effective search space used: 4000653125
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)