BLASTX 2.2.6 [Apr-09-2003] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 3106166.2.1 (715 letters) Database: /db/trembl-ebi/tmp/swall 1,175,531 sequences; 376,898,118 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sptr|Q9M0X9|Q9M0X9 Putative 4-coumarate--CoA ligase. 202 4e-51 sw|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 174 7e-43 sptr|Q9SWH8|Q9SWH8 4-coumarate:coenzyme A ligase (EC 6.2.1.12). 174 1e-42 sw|O24145|4CL1_TOBAC 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 173 2e-42 sw|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 172 4e-42 sw|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 172 4e-42 sw|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 172 5e-42 sptr|Q42943|Q42943 4-coumarate:coenzyme A ligase (EC 6.2.1.12). 171 8e-42 sw|P14912|4CL1_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 171 1e-41 sptrnew|AAP03021|AAP03021 4-coumarate-CoA ligase-like protein. 171 1e-41 sptr|Q9C5H2|Q9C5H2 Putative 4-coumarate:CoA ligase. 171 1e-41 sptr|Q8W558|Q8W558 4-coumarate:CoA ligase (EC 6.2.1.12). 170 1e-41 sptr|Q8LSQ3|Q8LSQ3 Putative AMP-binding protein. 169 2e-41 sptr|Q9FQY7|Q9FQY7 4-coumarate:coenzyme A ligase. 169 2e-41 sptr|Q8S5C2|Q8S5C2 4-coumarate:CoA ligase isoenzyme 3 (EC 6.2.1.... 168 5e-41 sw|O24540|4CL_VANPL 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) ... 168 5e-41 sptr|Q9LL49|Q9LL49 4-coumarate:CoA ligase 1 (EC 6.2.1.12). 168 5e-41 sptrnew|AAP03015|AAP03015 4-coumarate-CoA ligase-like protein. 167 9e-41 sptr|Q8GXU2|Q8GXU2 Putative 4-coumarate--CoA ligase. 167 9e-41 sptr|Q9FF44|Q9FF44 AMP-binding protein-like. 167 9e-41 sptr|Q941M4|Q941M4 4-coumarate:CoA ligase. 167 9e-41 sptr|Q941M3|Q941M3 4-coumarate:CoA ligase. 167 9e-41 sw|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 167 1e-40 sptr|Q8S5C1|Q8S5C1 4-coumarate:CoA ligase isoenzyme 2 (EC 6.2.1.... 167 1e-40 sw|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 167 1e-40 sptr|O81139|O81139 4-coumarate:CoA ligase (EC 6.2.1.12). 166 3e-40 sptr|Q94G19|Q94G19 4-coumarate:CoA ligase 3. 166 3e-40 sptr|Q8W2E0|Q8W2E0 4-coumarate:CoA ligase. 166 3e-40 sptr|Q9LQ12|Q9LQ12 F16P17.9 protein (4-coumarate-CoA ligase-like... 166 3e-40 sw|Q42982|4CL2_ORYSA 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 165 6e-40
>sptr|Q9M0X9|Q9M0X9 Putative 4-coumarate--CoA ligase. Length = 544 Score = 202 bits (513), Expect = 4e-51 Identities = 96/127 (75%), Positives = 115/127 (90%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++ W+HTGD+GYF+E G L+VVDR+KELIKYKGFQ+APAELEGLL+SHP+ILDAVVIPFP Sbjct: 416 KKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFP 475 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D EAGEVPIA+VVRS SS+TE D+QKFI KQVA YK+LRRV+F+ VPKSA+GKILRRE Sbjct: 476 DEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRE 535 Query: 327 LISKVRS 307 L+ +VRS Sbjct: 536 LVQQVRS 542 >sw|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 542 Score = 174 bits (442), Expect = 7e-43 Identities = 80/125 (64%), Positives = 107/125 (85%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWL+TGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP I DA V+P Sbjct: 412 KEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMK 471 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ FI KQV +YK+++RV FVD++PKS SGKILR++ Sbjct: 472 DEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKD 531 Query: 327 LISKV 313 L +K+ Sbjct: 532 LRAKL 536 >sptr|Q9SWH8|Q9SWH8 4-coumarate:coenzyme A ligase (EC 6.2.1.12). Length = 545 Score = 174 bits (440), Expect = 1e-42 Identities = 79/125 (63%), Positives = 108/125 (86%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 E+GWLHTGDIG+ D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HP+I DA V+P Sbjct: 415 EEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMI 474 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ FI KQV +YK+++RV FV++VPKS SGKILR++ Sbjct: 475 DEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKD 534 Query: 327 LISKV 313 L +++ Sbjct: 535 LRARL 539 >sw|O24145|4CL1_TOBAC 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 547 Score = 173 bits (438), Expect = 2e-42 Identities = 79/125 (63%), Positives = 107/125 (85%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIG+ DE +LF+VDRLKELIKYKGFQ+APAE+E LLL+HP I DA V+P Sbjct: 417 KEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMK 476 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ FI KQV +YK+++RV FV++VPKS SGKILR++ Sbjct: 477 DEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKD 536 Query: 327 LISKV 313 L +++ Sbjct: 537 LRARL 541 >sw|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 545 Score = 172 bits (436), Expect = 4e-42 Identities = 78/125 (62%), Positives = 108/125 (86%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIG+ D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HP+I DA V+P Sbjct: 415 KEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMI 474 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ FI KQV +YK+++RV FV++VPKS SGKILR++ Sbjct: 475 DEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKD 534 Query: 327 LISKV 313 L +++ Sbjct: 535 LRARL 539 >sw|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 545 Score = 172 bits (436), Expect = 4e-42 Identities = 78/125 (62%), Positives = 108/125 (86%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIG+ D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HP+I DA V+P Sbjct: 415 KEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMI 474 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ FI KQV +YK+++RV FV++VPKS SGKILR++ Sbjct: 475 DEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKD 534 Query: 327 LISKV 313 L +++ Sbjct: 535 LRARL 539 >sw|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 544 Score = 172 bits (435), Expect = 5e-42 Identities = 78/130 (60%), Positives = 107/130 (82%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 E+GWLHTGDIG+ D+ +LF+VDRLKE+IKYKGFQ+APAELE LLL+HP I DA V+P Sbjct: 413 EEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMI 472 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVR++ + TE ++++F+ KQV +YK++ RV FVD++PKS SGKILR++ Sbjct: 473 DEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 532 Query: 327 LISKVRSSKL 298 L +K+ S L Sbjct: 533 LRAKIASGDL 542 >sptr|Q42943|Q42943 4-coumarate:coenzyme A ligase (EC 6.2.1.12). Length = 542 Score = 171 bits (433), Expect = 8e-42 Identities = 80/125 (64%), Positives = 105/125 (84%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWL+TGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP DA V+P Sbjct: 412 KEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVPMK 471 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +A EVP+A+VVRSS S++TE +V+ FI KQV +YK+++RV FVD+VPKS SGKILR++ Sbjct: 472 DEQAEEVPVAFVVRSSGSTITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILRKD 531 Query: 327 LISKV 313 L +K+ Sbjct: 532 LRAKL 536 >sw|P14912|4CL1_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 544 Score = 171 bits (432), Expect = 1e-41 Identities = 77/130 (59%), Positives = 107/130 (82%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 E+GWLHTGDIG+ D+ +LF+VDRLKE+IKYKGFQ+APAELE LLL+HP I DA V+P Sbjct: 413 EEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMI 472 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVR++ + TE ++++F+ KQV +YK++ RV FVD++PKS SGKILR++ Sbjct: 473 DEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 532 Query: 327 LISKVRSSKL 298 L +++ S L Sbjct: 533 LRARIASGDL 542 >sptrnew|AAP03021|AAP03021 4-coumarate-CoA ligase-like protein. Length = 546 Score = 171 bits (432), Expect = 1e-41 Identities = 81/127 (63%), Positives = 102/127 (80%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWL TGD+ Y DE G +FVVDRLKELIKYKG+Q+APAELE LLL+HPEI DA VIPFPD Sbjct: 417 EGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPD 476 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 E G+ P+AYVVR + SSL+E + +F+ KQVA YK++R+V FV S+PK+ SGKILR++L Sbjct: 477 KEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDL 536 Query: 324 ISKVRSS 304 I S+ Sbjct: 537 IKIATSN 543 >sptr|Q9C5H2|Q9C5H2 Putative 4-coumarate:CoA ligase. Length = 546 Score = 171 bits (432), Expect = 1e-41 Identities = 81/127 (63%), Positives = 102/127 (80%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWL TGD+ Y DE G +FVVDRLKELIKYKG+Q+APAELE LLL+HPEI DA VIPFPD Sbjct: 417 EGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPD 476 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 E G+ P+AYVVR + SSL+E + +F+ KQVA YK++R+V FV S+PK+ SGKILR++L Sbjct: 477 KEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDL 536 Query: 324 ISKVRSS 304 I S+ Sbjct: 537 IKIATSN 543 >sptr|Q8W558|Q8W558 4-coumarate:CoA ligase (EC 6.2.1.12). Length = 540 Score = 170 bits (431), Expect = 1e-41 Identities = 79/126 (62%), Positives = 105/126 (83%), Gaps = 1/126 (0%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLLSHP+I DA V+P Sbjct: 409 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPKITDAAVVPMK 468 Query: 507 DAEAGEVPIAYVVRSS-ESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRR 331 D AGEVP+A+VVRS+ + TE ++++FI KQV +YK++ RV F+D++PKS SGKILR+ Sbjct: 469 DEAAGEVPVAFVVRSNGHTDTTEDEIKQFISKQVVFYKRISRVFFIDAIPKSPSGKILRK 528 Query: 330 ELISKV 313 +L +K+ Sbjct: 529 DLRAKL 534 >sptr|Q8LSQ3|Q8LSQ3 Putative AMP-binding protein. Length = 565 Score = 169 bits (429), Expect = 2e-41 Identities = 82/128 (64%), Positives = 103/128 (80%) Frame = -2 Query: 681 GWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDA 502 GWL TGD+ Y DE G LFVVDRLKELIKYKG+Q+ PAELE LLL+HPE+ D VIPFPD Sbjct: 439 GWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPDR 498 Query: 501 EAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRELI 322 E G+ P+AY+VR S+L+E +V +F+ KQVA YKK+R+V FV +PK+ASGKILR++LI Sbjct: 499 EVGQFPMAYIVRKKGSNLSEREVMEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDLI 558 Query: 321 SKVRSSKL 298 K+ +SKL Sbjct: 559 -KLATSKL 565 >sptr|Q9FQY7|Q9FQY7 4-coumarate:coenzyme A ligase. Length = 542 Score = 169 bits (429), Expect = 2e-41 Identities = 76/125 (60%), Positives = 106/125 (84%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GW+HTGD+G+ D +LF+VDRLKELIKYKGFQ+APAELE LLL+HP I DA V+P Sbjct: 412 KKGWVHTGDMGFIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMK 471 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VVRS+ S++TE +V+ F+ KQV +YK+++RV FV++VPKS SGKILR++ Sbjct: 472 DEQAGEVPVAFVVRSNGSTITEDEVKDFVSKQVVFYKRIKRVFFVETVPKSPSGKILRKD 531 Query: 327 LISKV 313 L +++ Sbjct: 532 LRARL 536 >sptr|Q8S5C2|Q8S5C2 4-coumarate:CoA ligase isoenzyme 3 (EC 6.2.1.12). Length = 570 Score = 168 bits (426), Expect = 5e-41 Identities = 78/127 (61%), Positives = 103/127 (81%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWLHTGD+GY DE ++F+VDR+KELIKYKGFQ+ PAELEGLL+SHP I DA V+P D Sbjct: 439 EGWLHTGDVGYVDEDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVVPQKD 498 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 AGEVP+A+VVRS+ LTE V++FI KQV +YK+L +V FV ++PKS SGKILR++L Sbjct: 499 VAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDL 558 Query: 324 ISKVRSS 304 +K+ ++ Sbjct: 559 RAKLETA 565 >sw|O24540|4CL_VANPL 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) (4-coumaroyl-CoA synthase). Length = 553 Score = 168 bits (426), Expect = 5e-41 Identities = 76/130 (58%), Positives = 108/130 (83%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP I DA V+P Sbjct: 421 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMK 480 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D AGEVP+A+VV+S+ ++TE ++++FI KQV +YK++ RV FV+++PK+ SGKILR++ Sbjct: 481 DEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKD 540 Query: 327 LISKVRSSKL 298 L +++ ++ L Sbjct: 541 LRARLAAAAL 550 >sptr|Q9LL49|Q9LL49 4-coumarate:CoA ligase 1 (EC 6.2.1.12). Length = 543 Score = 168 bits (426), Expect = 5e-41 Identities = 76/125 (60%), Positives = 103/125 (82%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 +QGWLHTGDIG+ D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HP I DA V+P Sbjct: 413 KQGWLHTGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLVTHPNISDAAVVPMK 472 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D AGEVP+A+VV S +TE ++++FI KQV +YK+++RV F++++PKS SGKILR+E Sbjct: 473 DDAAGEVPVAFVVSPKGSQITEDEIKQFISKQVVFYKRIKRVFFIEAIPKSPSGKILRKE 532 Query: 327 LISKV 313 L +K+ Sbjct: 533 LRAKL 537 >sptrnew|AAP03015|AAP03015 4-coumarate-CoA ligase-like protein. Length = 550 Score = 167 bits (424), Expect = 9e-41 Identities = 80/121 (66%), Positives = 97/121 (80%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWL TGD+ Y D G LF+VDRLKELIKYKG+Q+ PAELE LLL+HP+ILDA VIPFPD Sbjct: 423 EGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPD 482 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 EAG+ P+AYV R ES+L E V FI KQVA YKK+R+V F+DS+PK+ SGK LR++L Sbjct: 483 KEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDL 542 Query: 324 I 322 I Sbjct: 543 I 543 >sptr|Q8GXU2|Q8GXU2 Putative 4-coumarate--CoA ligase. Length = 550 Score = 167 bits (424), Expect = 9e-41 Identities = 80/121 (66%), Positives = 97/121 (80%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWL TGD+ Y D G LF+VDRLKELIKYKG+Q+ PAELE LLL+HP+ILDA VIPFPD Sbjct: 423 EGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPD 482 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 EAG+ P+AYV R ES+L E V FI KQVA YKK+R+V F+DS+PK+ SGK LR++L Sbjct: 483 KEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDL 542 Query: 324 I 322 I Sbjct: 543 I 543 >sptr|Q9FF44|Q9FF44 AMP-binding protein-like. Length = 544 Score = 167 bits (424), Expect = 9e-41 Identities = 80/121 (66%), Positives = 97/121 (80%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWL TGD+ Y D G LF+VDRLKELIKYKG+Q+ PAELE LLL+HP+ILDA VIPFPD Sbjct: 417 EGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPD 476 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 EAG+ P+AYV R ES+L E V FI KQVA YKK+R+V F+DS+PK+ SGK LR++L Sbjct: 477 KEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDL 536 Query: 324 I 322 I Sbjct: 537 I 537 >sptr|Q941M4|Q941M4 4-coumarate:CoA ligase. Length = 536 Score = 167 bits (424), Expect = 9e-41 Identities = 74/125 (59%), Positives = 104/125 (83%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HPEI DA V+ Sbjct: 409 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLK 468 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VV+S +S TE +++++I KQV +YK+++RV F++++PK+ SGKILR+ Sbjct: 469 DEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKN 528 Query: 327 LISKV 313 L K+ Sbjct: 529 LKEKL 533 >sptr|Q941M3|Q941M3 4-coumarate:CoA ligase. Length = 536 Score = 167 bits (424), Expect = 9e-41 Identities = 74/125 (59%), Positives = 104/125 (83%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HPEI DA V+ Sbjct: 409 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLK 468 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VV+S +S TE +++++I KQV +YK+++RV F++++PK+ SGKILR+ Sbjct: 469 DEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKN 528 Query: 327 LISKV 313 L K+ Sbjct: 529 LKEKL 533 >sw|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2) (Clone 4CL16). Length = 562 Score = 167 bits (423), Expect = 1e-40 Identities = 77/127 (60%), Positives = 103/127 (81%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWLHTGD+GY D+ ++F+VDR+KELIKYKGFQ+ PAELEGLL+SHP I DA V+P D Sbjct: 431 EGWLHTGDVGYVDDDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVVPQKD 490 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 AGEVP+A+VVRS+ LTE V++FI KQV +YK+L +V FV ++PKS SGKILR++L Sbjct: 491 VAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDL 550 Query: 324 ISKVRSS 304 +K+ ++ Sbjct: 551 RAKLETA 557 >sptr|Q8S5C1|Q8S5C1 4-coumarate:CoA ligase isoenzyme 2 (EC 6.2.1.12). Length = 547 Score = 167 bits (422), Expect = 1e-40 Identities = 78/129 (60%), Positives = 105/129 (81%), Gaps = 1/129 (0%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 + GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP+I DA V+P Sbjct: 416 KDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVPMK 475 Query: 507 DAEAGEVPIAYVVRSS-ESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRR 331 D AGEVP+A+VV S+ + TE ++++FI KQV +YK++ RV F+D++PKS SGKILR+ Sbjct: 476 DEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 535 Query: 330 ELISKVRSS 304 +L +K+ +S Sbjct: 536 DLRAKIAAS 544 >sw|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) (Clone 4CL14) (Fragment). Length = 293 Score = 167 bits (422), Expect = 1e-40 Identities = 78/129 (60%), Positives = 105/129 (81%), Gaps = 1/129 (0%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 + GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP+I DA V+P Sbjct: 162 KDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVPMK 221 Query: 507 DAEAGEVPIAYVVRSS-ESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRR 331 D AGEVP+A+VV S+ + TE ++++FI KQV +YK++ RV F+D++PKS SGKILR+ Sbjct: 222 DEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 281 Query: 330 ELISKVRSS 304 +L +K+ +S Sbjct: 282 DLRAKIAAS 290 >sptr|O81139|O81139 4-coumarate:CoA ligase (EC 6.2.1.12). Length = 535 Score = 166 bits (420), Expect = 3e-40 Identities = 73/125 (58%), Positives = 103/125 (82%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+AP ELE LL++HPEI DA V+ Sbjct: 408 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPTELEALLIAHPEISDAAVVGLK 467 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VV+S +S TE +++++I KQV +YK+++RV F++++PK+ SGKILR+ Sbjct: 468 DEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKN 527 Query: 327 LISKV 313 L K+ Sbjct: 528 LKEKL 532 >sptr|Q94G19|Q94G19 4-coumarate:CoA ligase 3. Length = 540 Score = 166 bits (420), Expect = 3e-40 Identities = 73/121 (60%), Positives = 102/121 (84%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LLL+HP+I DA V+ Sbjct: 413 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLAHPQISDAAVVGMK 472 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+VV+S +S TE +++++I KQV +YK+++RV F++++PK+ SGKILR+ Sbjct: 473 DEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKN 532 Query: 327 L 325 L Sbjct: 533 L 533 >sptr|Q8W2E0|Q8W2E0 4-coumarate:CoA ligase. Length = 536 Score = 166 bits (420), Expect = 3e-40 Identities = 73/125 (58%), Positives = 103/125 (82%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 ++GWLHTGDIGY D+ +LF+VDRLKELIKYKGFQ+APAELE LL++HPEI DA V+ Sbjct: 409 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGMK 468 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D +AGEVP+A+ V+S +S TE +++++I KQV +YK+++RV F++++PK+ SGKILR+ Sbjct: 469 DEDAGEVPVAFAVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKN 528 Query: 327 LISKV 313 L K+ Sbjct: 529 LKEKL 533 >sptr|Q9LQ12|Q9LQ12 F16P17.9 protein (4-coumarate-CoA ligase-like protein). Length = 542 Score = 166 bits (419), Expect = 3e-40 Identities = 77/127 (60%), Positives = 98/127 (77%) Frame = -2 Query: 687 EQGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFP 508 EQGWLHTGDIGY D+ G +F+VDR+KELIKYKGFQ+APAELE +LL+HP + D V+P P Sbjct: 412 EQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLP 471 Query: 507 DAEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRE 328 D EAGE+P A VV + +++ E D+ F+ VA+YKK+R V FVDS+PKS SGKI+RR Sbjct: 472 DEEAGEIPAACVVINPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRL 531 Query: 327 LISKVRS 307 L K+ S Sbjct: 532 LRDKILS 538 >sw|Q42982|4CL2_ORYSA 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 569 Score = 165 bits (417), Expect = 6e-40 Identities = 74/124 (59%), Positives = 103/124 (83%) Frame = -2 Query: 684 QGWLHTGDIGYFDEGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 505 +GWLHTG+IGY D+ ++F+VDR+KELIK+KGFQ+ PAELE LL++HP I DA V+P D Sbjct: 443 EGWLHTGNIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLIAHPSIRDAAVVPQKD 502 Query: 504 AEAGEVPIAYVVRSSESSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRREL 325 AGEVP+A+VVR+++S +TE +++FI KQV +YK+L +V F+ ++PKSASGKILRREL Sbjct: 503 DVAGEVPVAFVVRAADSDITEESIKEFISKQVVFYKRLHKVHFIHAIPKSASGKILRREL 562 Query: 324 ISKV 313 +K+ Sbjct: 563 RAKL 566 Database: /db/trembl-ebi/tmp/swall Posted date: Jun 27, 2003 8:33 PM Number of letters in database: 376,898,118 Number of sequences in database: 1,175,531 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 470,472,024 Number of Sequences: 1175531 Number of extensions: 9349429 Number of successful extensions: 27834 Number of sequences better than 10.0: 30 Number of HSP's better than 10.0 without gapping: 26013 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 27228 length of database: 376,898,118 effective HSP length: 119 effective length of database: 237,009,929 effective search space used: 27967171622 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
S1: 41 (21.7 bits)