BLASTX 2.2.6 [Apr-09-2003] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 2437842.2.1 (1093 letters) Database: /db/trembl-ebi/tmp/swall 1,175,531 sequences; 376,898,118 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sptr|Q9M7S2|Q9M7S2 4-coumarate--CoA ligase 4CL2 (EC 6.2.1.12). 478 e-134 sptr|Q9M7S1|Q9M7S1 4-coumarate--CoA ligase 4CL3 (EC 6.2.1.12). 457 e-127 sptr|Q9LL50|Q9LL50 4-coumarate:CoA ligase 2 (EC 6.2.1.12). 421 e-116 sw|O24145|4CL1_TOBAC 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 412 e-114 sw|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 410 e-113 sw|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 409 e-113 sw|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 409 e-113 sw|O24540|4CL_VANPL 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) ... 406 e-112 sptr|O48869|O48869 4-coumarate:CoA ligase 1. 405 e-112 sptr|Q42943|Q42943 4-coumarate:coenzyme A ligase (EC 6.2.1.12). 404 e-112 sptr|Q9SWH8|Q9SWH8 4-coumarate:coenzyme A ligase (EC 6.2.1.12). 403 e-111 sptrnew|AAN15615|AAN15615 Putative 4-coumarate:CoA ligase 2. 403 e-111 sptrnew|AAM20546|AAM20546 Putative 4-coumarate:CoA ligase 2. 403 e-111 sw|Q9S725|4CL2_ARATH 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4C... 403 e-111 sw|P14912|4CL1_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 402 e-111 sw|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 401 e-111 sptr|Q42879|Q42879 4-coumarate:CoA ligase (EC 6.2.1.12). 398 e-110 sptr|O48868|O48868 4-coumarate:CoA ligase 2. 396 e-109 sptrnew|AAM20598|AAM20598 4-coumarate:CoA ligase 1. 395 e-109 sw|Q42524|4CL1_ARATH 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 395 e-109 sw|P17814|4CL1_ORYSA 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 395 e-109 sptr|Q941M4|Q941M4 4-coumarate:CoA ligase. 392 e-108 sptr|Q941M3|Q941M3 4-coumarate:CoA ligase. 392 e-108 sptr|Q9LL49|Q9LL49 4-coumarate:CoA ligase 1 (EC 6.2.1.12). 392 e-108 sptr|Q8S5C1|Q8S5C1 4-coumarate:CoA ligase isoenzyme 2 (EC 6.2.1.... 390 e-107 sw|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4C... 390 e-107 sptr|Q8W558|Q8W558 4-coumarate:CoA ligase (EC 6.2.1.12). 390 e-107 sptr|Q8W2E0|Q8W2E0 4-coumarate:CoA ligase. 389 e-107 sptr|Q94G19|Q94G19 4-coumarate:CoA ligase 3. 389 e-107 sptr|O81139|O81139 4-coumarate:CoA ligase (EC 6.2.1.12). 388 e-107
>sptr|Q9M7S2|Q9M7S2 4-coumarate--CoA ligase 4CL2 (EC 6.2.1.12). Length = 556 Score = 478 bits (1229), Expect = e-134 Identities = 240/261 (91%), Positives = 252/261 (96%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS RV AADLASIR+VMSGAAPMGK+LQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA Sbjct: 296 KSARVTAADLASIRLVMSGAAPMGKELQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 355 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSGSCGTVVRNAELKIVDPDT ASLGRN PGEICIRG+QIMKGYLNDP ATKNT Sbjct: 356 KEPFAVKSGSCGTVVRNAELKIVDPDTGASLGRNLPGEICIRGKQIMKGYLNDPVATKNT 415 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS Sbjct: 416 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 475 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M+DE+AGEVPVAF++R+EGSEISENEIKQFVAKEVVFYKRI +VFF DSIPK+PSGKILR Sbjct: 476 MQDELAGEVPVAFVVRTEGSEISENEIKQFVAKEVVFYKRICKVFFADSIPKSPSGKILR 535 Query: 727 KDLRARLAAGIPSSDNTQSKS 789 KDLRA+LAAGIPSS+ TQSKS Sbjct: 536 KDLRAKLAAGIPSSNTTQSKS 556 >sptr|Q9M7S1|Q9M7S1 4-coumarate--CoA ligase 4CL3 (EC 6.2.1.12). Length = 557 Score = 457 bits (1175), Expect = e-127 Identities = 226/253 (89%), Positives = 243/253 (96%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS RVGA DLASIRMV+SGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA Sbjct: 298 KSDRVGADDLASIRMVLSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 357 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF+VKSGSCGTVVRNAELK+VDPDT ASLGRNQPGEIC+RG+QIM GYLNDPE+TKNT Sbjct: 358 KEPFKVKSGSCGTVVRNAELKVVDPDTGASLGRNQPGEICVRGKQIMIGYLNDPESTKNT 417 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIG VDDDDEIFIVDRLKEIIKYKGFQV PAELEALL+T+PE+KDAAVV Sbjct: 418 IDKDGWLHTGDIGLVDDDDEIFIVDRLKEIIKYKGFQVAPAELEALLLTNPEVKDAAVVG 477 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 +KD++ GEVPVAFI R EGSEI+ENEIKQFV+KEVVFYKRIN+V+FTDSIPKNPSGKILR Sbjct: 478 VKDDLCGEVPVAFIKRIEGSEINENEIKQFVSKEVVFYKRINKVYFTDSIPKNPSGKILR 537 Query: 727 KDLRARLAAGIPS 765 KDLRARLAAGIP+ Sbjct: 538 KDLRARLAAGIPT 550 >sptr|Q9LL50|Q9LL50 4-coumarate:CoA ligase 2 (EC 6.2.1.12). Length = 544 Score = 421 bits (1081), Expect = e-116 Identities = 200/253 (79%), Positives = 233/253 (92%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 K P + DL+SIRMVMSGAAPMGK+L+D AK+PNA LGQGYGMTEAGPVL+MCLAFA Sbjct: 292 KCPDLHRYDLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFA 351 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP+E+KSG+CGTVVRNAE+KI+DPDT+ SL RNQ GEICIRG QIMKGYLNDPEAT+NT Sbjct: 352 KEPYEIKSGACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENT 411 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEA+LI+HP + DAAVVS Sbjct: 412 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVS 471 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF++RS GS+ISE++IKQ+++K+VVFYKRI++VFFTD IPK PSGKILR Sbjct: 472 MKDEAAGEVPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILR 531 Query: 727 KDLRARLAAGIPS 765 KDLRARLAAG+P+ Sbjct: 532 KDLRARLAAGLPN 544 >sw|O24145|4CL1_TOBAC 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 547 Score = 412 bits (1059), Expect = e-114 Identities = 193/253 (76%), Positives = 228/253 (90%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V + DL+S+R VMSGAAP+GK+L+DA K PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 295 KSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFA 354 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++KSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 355 KEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRT 414 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIG++D+DDE+FIVDRLKE+IKYKGFQV PAE+EALL+ HP I DAAVV Sbjct: 415 IDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVP 474 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF++RS GS I+E+E+K F++K+V+FYKR+ RVFF +++PK+PSGKILR Sbjct: 475 MKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILR 534 Query: 727 KDLRARLAAGIPS 765 KDLRARLAAG+P+ Sbjct: 535 KDLRARLAAGVPN 547 >sw|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 545 Score = 410 bits (1053), Expect = e-113 Identities = 196/251 (78%), Positives = 226/251 (90%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 293 KSPLVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 352 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++KSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 353 KEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATART 412 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 I+K+GWLHTGDIG++DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HP+I DAAVV Sbjct: 413 IEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVP 472 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M DE AGEVPVAF++RS GS I+E+E+K F++K+V+FYKRI RVFF +++PK+PSGKILR Sbjct: 473 MIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILR 532 Query: 727 KDLRARLAAGI 759 KDLRARLAAGI Sbjct: 533 KDLRARLAAGI 543 >sw|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 545 Score = 409 bits (1052), Expect = e-113 Identities = 196/251 (78%), Positives = 226/251 (90%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 293 KSPLVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 352 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++KSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 353 KEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATART 412 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 I+K+GWLHTGDIG++DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HP+I DAAVV Sbjct: 413 IEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVP 472 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M DE AGEVPVAF++RS GS I+E+E+K F++K+V+FYKRI RVFF +++PK+PSGKILR Sbjct: 473 MIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILR 532 Query: 727 KDLRARLAAGI 759 KDLRARLAAGI Sbjct: 533 KDLRARLAAGI 543 >sw|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 542 Score = 409 bits (1050), Expect = e-113 Identities = 196/253 (77%), Positives = 225/253 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+D AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 290 KSPMVDDYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 349 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFE+KSG+CGTVVRNAE+KIVDP T SL RNQ GEICIRG+QIMKGYLNDPEAT T Sbjct: 350 KEPFEIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATART 409 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWL+TGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+ HP I DAAVV Sbjct: 410 IDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVP 469 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF++RS GS I+E+E+K F++K+V+FYKRI RVFF D+IPK+PSGKILR Sbjct: 470 MKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILR 529 Query: 727 KDLRARLAAGIPS 765 KDLRA+LAAG+P+ Sbjct: 530 KDLRAKLAAGLPN 542 >sw|O24540|4CL_VANPL 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) (4-coumaroyl-CoA synthase). Length = 553 Score = 406 bits (1044), Expect = e-112 Identities = 196/249 (78%), Positives = 224/249 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 299 KSTVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 358 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++KSG+CGTVVRNAE+KIVDP+T +SL RN PGEICIRG+QIMKGYLNDPEAT T Sbjct: 359 KEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATART 418 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+THP I DAAVV Sbjct: 419 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVP 478 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF+++S G I+E+EIKQF++K+V+FYKRINRVFF ++IPK PSGKILR Sbjct: 479 MKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILR 538 Query: 727 KDLRARLAA 753 KDLRARLAA Sbjct: 539 KDLRARLAA 547 >sptr|O48869|O48869 4-coumarate:CoA ligase 1. Length = 557 Score = 405 bits (1042), Expect = e-112 Identities = 194/250 (77%), Positives = 223/250 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 K P V DL+SIR VMSGAAPMGK+L+D AK+PNA LGQGYGMTEAGPVL+MCLAFA Sbjct: 292 KCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFA 351 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFE+KSG+CGTVVRNAE+KIVDPDT SL RNQ GEICIRG QIMKGYLNDPEAT+ T Sbjct: 352 KEPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQSGEICIRGSQIMKGYLNDPEATERT 411 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 +D DGWLHTGDIGY+D DDE+FIVDRLKE+IKYKGFQV PAELEA+LI HP+I D AVV Sbjct: 412 VDNDGWLHTGDIGYIDGDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVP 471 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVP+AF++R+ GS+I+E+EIKQ+++K+VVFYKRI+RVFFT++IPK PSGKILR Sbjct: 472 MKDEAAGEVPIAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPSGKILR 531 Query: 727 KDLRARLAAG 756 KDLRARLA G Sbjct: 532 KDLRARLATG 541 >sptr|Q42943|Q42943 4-coumarate:coenzyme A ligase (EC 6.2.1.12). Length = 542 Score = 404 bits (1039), Expect = e-112 Identities = 193/253 (76%), Positives = 224/253 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+D AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 290 KSPMVDDYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 349 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFE+KSG+CGTVVRNAE+KIVDP+T SL RNQ GEICIRG+QIMKGYLNDPEAT T Sbjct: 350 KEPFEIKSGACGTVVRNAEMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDPEATART 409 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWL+TGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+ HP DAAVV Sbjct: 410 IDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVP 469 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE A EVPVAF++RS GS I+E+E+K F++K+V+FYKRI RVFF D++PK+PSGKILR Sbjct: 470 MKDEQAEEVPVAFVVRSSGSTITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILR 529 Query: 727 KDLRARLAAGIPS 765 KDLRA+LAAG+P+ Sbjct: 530 KDLRAKLAAGLPN 542 >sptr|Q9SWH8|Q9SWH8 4-coumarate:coenzyme A ligase (EC 6.2.1.12). Length = 545 Score = 403 bits (1036), Expect = e-111 Identities = 193/251 (76%), Positives = 224/251 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAG VL MCLAFA Sbjct: 293 KSPLVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGTVLTMCLAFA 352 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++KSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 353 KEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATART 412 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 I+++GWLHTGDIG++DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HP+I DAAVV Sbjct: 413 IEEEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVP 472 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M DE AGEVPVAF++RS GS I+E+E+K F++K+V+FYKRI RVFF +++PK+PSGKILR Sbjct: 473 MIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILR 532 Query: 727 KDLRARLAAGI 759 KDLRARLAAGI Sbjct: 533 KDLRARLAAGI 543 >sptrnew|AAN15615|AAN15615 Putative 4-coumarate:CoA ligase 2. Length = 556 Score = 403 bits (1035), Expect = e-111 Identities = 197/251 (78%), Positives = 223/251 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP DL+S+RMV SGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAM L FA Sbjct: 304 KSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFA 363 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSG+CGTVVRNAE+KI+DPDT SL RN+PGEICIRG QIMKGYLNDP AT +T Sbjct: 364 KEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATAST 423 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGD+G++DDDDE+FIVDRLKE+IKYKGFQV PAELE+LLI HPEI D AVV+ Sbjct: 424 IDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVA 483 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MK+E AGEVPVAF++RS+ S ISE+EIKQFV+K+VVFYKRIN+VFFTDSIPK PSGKILR Sbjct: 484 MKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILR 543 Query: 727 KDLRARLAAGI 759 KDLRARLA G+ Sbjct: 544 KDLRARLANGL 554 >sptrnew|AAM20546|AAM20546 Putative 4-coumarate:CoA ligase 2. Length = 556 Score = 403 bits (1035), Expect = e-111 Identities = 197/251 (78%), Positives = 223/251 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP DL+S+RMV SGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAM L FA Sbjct: 304 KSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFA 363 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSG+CGTVVRNAE+KI+DPDT SL RN+PGEICIRG QIMKGYLNDP AT +T Sbjct: 364 KEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATAST 423 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGD+G++DDDDE+FIVDRLKE+IKYKGFQV PAELE+LLI HPEI D AVV+ Sbjct: 424 IDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVA 483 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MK+E AGEVPVAF++RS+ S ISE+EIKQFV+K+VVFYKRIN+VFFTDSIPK PSGKILR Sbjct: 484 MKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILR 543 Query: 727 KDLRARLAAGI 759 KDLRARLA G+ Sbjct: 544 KDLRARLANGL 554 >sw|Q9S725|4CL2_ARATH 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2). Length = 556 Score = 403 bits (1035), Expect = e-111 Identities = 197/251 (78%), Positives = 223/251 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP DL+S+RMV SGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAM L FA Sbjct: 304 KSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFA 363 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSG+CGTVVRNAE+KI+DPDT SL RN+PGEICIRG QIMKGYLNDP AT +T Sbjct: 364 KEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATAST 423 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGD+G++DDDDE+FIVDRLKE+IKYKGFQV PAELE+LLI HPEI D AVV+ Sbjct: 424 IDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVA 483 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MK+E AGEVPVAF++RS+ S ISE+EIKQFV+K+VVFYKRIN+VFFTDSIPK PSGKILR Sbjct: 484 MKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILR 543 Query: 727 KDLRARLAAGI 759 KDLRARLA G+ Sbjct: 544 KDLRARLANGL 554 >sw|P14912|4CL1_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 544 Score = 402 bits (1033), Expect = e-111 Identities = 195/250 (78%), Positives = 225/250 (90%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 291 KSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 350 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP+E+KSG+CGTVVRNAE+KIVDP+T+ASL RNQ GEICIRG+QIMKGYLNDPE+T+ T Sbjct: 351 KEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTT 410 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 ID++GWLHTGDIG++DDDDE+FIVDRLKEIIKYKGFQV PAELEALL+THP I DAAVV Sbjct: 411 IDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVP 470 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M DE AGEVPVAF++R+ G +E EIKQFV+K+VVFYKRI RVFF D+IPK+PSGKILR Sbjct: 471 MIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILR 530 Query: 727 KDLRARLAAG 756 KDLRAR+A+G Sbjct: 531 KDLRARIASG 540 >sw|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 544 Score = 401 bits (1030), Expect = e-111 Identities = 194/250 (77%), Positives = 225/250 (90%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+S+R VMSGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 291 KSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 350 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP+E+KSG+CGTVVRNAE+KIVDP+T+ASL RNQ GEICIRG+QIMKGYLNDPE+T+ T Sbjct: 351 KEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTT 410 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 ID++GWLHTGDIG++DDDDE+FIVDRLKEIIKYKGFQV PAELEALL+THP I DAAVV Sbjct: 411 IDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVP 470 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 M DE AGEVPVAF++R+ G +E EIKQFV+K+VVFYKRI RVFF D+IPK+PSGKILR Sbjct: 471 MIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILR 530 Query: 727 KDLRARLAAG 756 KDLRA++A+G Sbjct: 531 KDLRAKIASG 540 >sptr|Q42879|Q42879 4-coumarate:CoA ligase (EC 6.2.1.12). Length = 636 Score = 398 bits (1022), Expect = e-110 Identities = 192/249 (77%), Positives = 222/249 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS V DL+++R VMSGAAP+G +L+DA AK PNA LGQGYGMTEAGPVLAMCLAFA Sbjct: 293 KSNVVDQYDLSTVRTVMSGAAPLGSELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 352 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFE+KSG+CGTVVRN+E+KI+D +T ASL RNQ GEICIRG+QIMKGYLNDPEAT+ T Sbjct: 353 KEPFEIKSGACGTVVRNSEMKIIDTETGASLPRNQSGEICIRGDQIMKGYLNDPEATERT 412 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV P ELEALL+ HP + DAAVVS Sbjct: 413 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPPELEALLVPHPNVSDAAVVS 472 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF++RS GS +E+EIKQFV+K+V+FYKRINRVF DSIPK+PSGKI+R Sbjct: 473 MKDEGAGEVPVAFVVRSNGSTTTEDEIKQFVSKQVIFYKRINRVFGVDSIPKSPSGKIVR 532 Query: 727 KDLRARLAA 753 KDLRA+LAA Sbjct: 533 KDLRAKLAA 541 >sptr|O48868|O48868 4-coumarate:CoA ligase 2. Length = 548 Score = 396 bits (1017), Expect = e-109 Identities = 192/250 (76%), Positives = 224/250 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP V DL+SIR V+SGAAP+GK+L+D AK+PNA LGQGYGMTEAGPV+AMCLAFA Sbjct: 292 KSPVVDQYDLSSIRTVLSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVIAMCLAFA 351 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFE+KSG+CGTVVRNAE+KIVDP+T S RN+ GEICIRG QIMKGYLNDPEAT+ T Sbjct: 352 KEPFEIKSGACGTVVRNAEMKIVDPETGESQPRNKTGEICIRGCQIMKGYLNDPEATERT 411 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIGY+ D+DE+FIVDRLKE+IKYKGFQV PAELEA+LI HP I DAAVV Sbjct: 412 IDKDGWLHTGDIGYI-DEDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVP 470 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF++RS GS+I+E+EIKQ+++K+V+FYKRI RVFFT++IPK PSGKILR Sbjct: 471 MKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSGKILR 530 Query: 727 KDLRARLAAG 756 KDLRAR++AG Sbjct: 531 KDLRARVSAG 540 >sptrnew|AAM20598|AAM20598 4-coumarate:CoA ligase 1. Length = 561 Score = 395 bits (1015), Expect = e-109 Identities = 193/251 (76%), Positives = 221/251 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS DL+SIR+V SGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAM L FA Sbjct: 311 KSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFA 370 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG QIMKGYLN+P AT T Sbjct: 371 KEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAET 430 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIG +DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HP+I D AVV+ Sbjct: 431 IDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVA 490 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MK+E AGEVPVAF+++S+ SE+SE+++KQFV+K+VVFYKRIN+VFFT+SIPK PSGKILR Sbjct: 491 MKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILR 550 Query: 727 KDLRARLAAGI 759 KDLRA+LA G+ Sbjct: 551 KDLRAKLANGL 561 >sw|Q42524|4CL1_ARATH 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 561 Score = 395 bits (1015), Expect = e-109 Identities = 193/251 (76%), Positives = 221/251 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS DL+SIR+V SGAAP+GK+L+DA AK PNA LGQGYGMTEAGPVLAM L FA Sbjct: 311 KSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFA 370 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF VKSG+CGTVVRNAE+KIVDPDT SL RNQPGEICIRG QIMKGYLN+P AT T Sbjct: 371 KEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAET 430 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIG +DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HP+I D AVV+ Sbjct: 431 IDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVA 490 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MK+E AGEVPVAF+++S+ SE+SE+++KQFV+K+VVFYKRIN+VFFT+SIPK PSGKILR Sbjct: 491 MKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILR 550 Query: 727 KDLRARLAAGI 759 KDLRA+LA G+ Sbjct: 551 KDLRAKLANGL 561 >sw|P17814|4CL1_ORYSA 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1). Length = 563 Score = 395 bits (1014), Expect = e-109 Identities = 192/247 (77%), Positives = 220/247 (89%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KS A DL+S+RMV+SGAAPMGKD++DAFMAK+P AVLGQGYGMTEAGPVL+MCLAFA Sbjct: 310 KSEAAAARDLSSVRMVLSGAAPMGKDIEDAFMAKLPGAVLGQGYGMTEAGPVLSMCLAFA 369 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF+VKSG+CGTVVRNAELKI+DPDT SLGRN GEICIRG+QIMKGYLN+PEATKNT Sbjct: 370 KEPFKVKSGACGTVVRNAELKIIDPDTGKSLGRNLRGEICIRGQQIMKGYLNNPEATKNT 429 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 ID +GWLHTGDIGYVDDDDEIFIVDRLKEIIKY+GFQV PAELEALL THP I DAAVV Sbjct: 430 IDAEGWLHTGDIGYVDDDDEIFIVDRLKEIIKYRGFQVAPAELEALLNTHPSIADAAVVG 489 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 +K GE+PVAF+ ++EGSE+SE+++KQFVAKEV++YK+I VFF D IPK PSGKILR Sbjct: 490 LK---FGEIPVAFVAKTEGSELSEDDVKQFVAKEVIYYKKIREVFFVDKIPKAPSGKILR 546 Query: 727 KDLRARL 747 K+LR +L Sbjct: 547 KELRKQL 553 >sptr|Q941M4|Q941M4 4-coumarate:CoA ligase. Length = 536 Score = 392 bits (1008), Expect = e-108 Identities = 186/248 (75%), Positives = 220/248 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+S+RM+ SG AP+GK+L+D AK P A LGQGYGMTEAGPVLAMCLAFA Sbjct: 287 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFA 346 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++K G+CGTVVRNAE+KIVDP+T ASL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 347 KEPFDIKPGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRT 406 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HPEI DAAVV Sbjct: 407 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVG 466 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 +KDE AGEVPVAF+++SE S+ +E+EIKQ+++K+V+FYKRI RVFF ++IPK PSGKILR Sbjct: 467 LKDEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILR 526 Query: 727 KDLRARLA 750 K+L+ +LA Sbjct: 527 KNLKEKLA 534 >sptr|Q941M3|Q941M3 4-coumarate:CoA ligase. Length = 536 Score = 392 bits (1008), Expect = e-108 Identities = 186/248 (75%), Positives = 220/248 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+S+RM+ SG AP+GK+L+D AK P A LGQGYGMTEAGPVLAMCLAFA Sbjct: 287 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFA 346 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++K G+CGTVVRNAE+KIVDP+T ASL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 347 KEPFDIKPGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRT 406 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HPEI DAAVV Sbjct: 407 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVG 466 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 +KDE AGEVPVAF+++SE S+ +E+EIKQ+++K+V+FYKRI RVFF ++IPK PSGKILR Sbjct: 467 LKDEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILR 526 Query: 727 KDLRARLA 750 K+L+ +LA Sbjct: 527 KNLKEKLA 534 >sptr|Q9LL49|Q9LL49 4-coumarate:CoA ligase 1 (EC 6.2.1.12). Length = 543 Score = 392 bits (1006), Expect = e-108 Identities = 188/250 (75%), Positives = 217/250 (86%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 K P + DL SIR++ SG AP+GK+L+D AK PN LGQGYGMTEAGPVL M LAFA Sbjct: 291 KFPDLDKYDLGSIRVLKSGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFA 350 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPFEVK G CGTVVRNAELKIVDP+T ASL RN PGEICIRG QIMKGYLNDPEAT+ T Sbjct: 351 KEPFEVKPGGCGTVVRNAELKIVDPETGASLPRNHPGEICIRGHQIMKGYLNDPEATRTT 410 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK GWLHTGDIG++DDD+E+FIVDRLKE+IKYKGFQV PAELEALL+THP I DAAVV Sbjct: 411 IDKQGWLHTGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLVTHPNISDAAVVP 470 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKD+ AGEVPVAF++ +GS+I+E+EIKQF++K+VVFYKRI RVFF ++IPK+PSGKILR Sbjct: 471 MKDDAAGEVPVAFVVSPKGSQITEDEIKQFISKQVVFYKRIKRVFFIEAIPKSPSGKILR 530 Query: 727 KDLRARLAAG 756 K+LRA+LAAG Sbjct: 531 KELRAKLAAG 540 >sptr|Q8S5C1|Q8S5C1 4-coumarate:CoA ligase isoenzyme 2 (EC 6.2.1.12). Length = 547 Score = 390 bits (1003), Expect = e-107 Identities = 190/253 (75%), Positives = 222/253 (87%), Gaps = 1/253 (0%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+SIR++ SG AP+GK+L+D AK PNA LGQGYGMTEAGPVL M LAFA Sbjct: 294 KSPDLHKYDLSSIRVLKSGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFA 353 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP +VK G+CGTVVRNAE+KIVDP+T SL RNQ GEICIRG+QIMKGYLND EAT+ T Sbjct: 354 KEPIDVKPGACGTVVRNAEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERT 413 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+THP+I DAAVV Sbjct: 414 IDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVP 473 Query: 547 MKDEVAGEVPVAFIIRSEG-SEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKIL 723 MKDE AGEVPVAF++ S G ++ +E+EIKQF++K+VVFYKRINRVFF D+IPK+PSGKIL Sbjct: 474 MKDEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKIL 533 Query: 724 RKDLRARLAAGIP 762 RKDLRA++AA +P Sbjct: 534 RKDLRAKIAASVP 546 >sw|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) (Clone 4CL14) (Fragment). Length = 293 Score = 390 bits (1003), Expect = e-107 Identities = 190/253 (75%), Positives = 222/253 (87%), Gaps = 1/253 (0%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+SIR++ SG AP+GK+L+D AK PNA LGQGYGMTEAGPVL M LAFA Sbjct: 40 KSPDLHKYDLSSIRVLKSGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFA 99 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP +VK G+CGTVVRNAE+KIVDP+T SL RNQ GEICIRG+QIMKGYLND EAT+ T Sbjct: 100 KEPIDVKPGACGTVVRNAEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERT 159 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDKDGWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+THP+I DAAVV Sbjct: 160 IDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVP 219 Query: 547 MKDEVAGEVPVAFIIRSEG-SEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKIL 723 MKDE AGEVPVAF++ S G ++ +E+EIKQF++K+VVFYKRINRVFF D+IPK+PSGKIL Sbjct: 220 MKDEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKIL 279 Query: 724 RKDLRARLAAGIP 762 RKDLRA++AA +P Sbjct: 280 RKDLRAKIAASVP 292 >sptr|Q8W558|Q8W558 4-coumarate:CoA ligase (EC 6.2.1.12). Length = 540 Score = 390 bits (1002), Expect = e-107 Identities = 189/254 (74%), Positives = 224/254 (88%), Gaps = 1/254 (0%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+SIR++ SG A +GK+L+D AK P A LGQGYGMTEAGPVL MCLAFA Sbjct: 287 KSPDLEKYDLSSIRVLKSGGASLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLAFA 346 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEP +VK G+CGTVVRNAE+KIVDP+T SL RNQ GEICIRG+QIMKGYLND EAT+ T Sbjct: 347 KEPIDVKPGACGTVVRNAEMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDQEATQRT 406 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL++HP+I DAAVV Sbjct: 407 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPKITDAAVVP 466 Query: 547 MKDEVAGEVPVAFIIRSEG-SEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKIL 723 MKDE AGEVPVAF++RS G ++ +E+EIKQF++K+VVFYKRI+RVFF D+IPK+PSGKIL Sbjct: 467 MKDEAAGEVPVAFVVRSNGHTDTTEDEIKQFISKQVVFYKRISRVFFIDAIPKSPSGKIL 526 Query: 724 RKDLRARLAAGIPS 765 RKDLRA+LAAG+P+ Sbjct: 527 RKDLRAKLAAGVPN 540 >sptr|Q8W2E0|Q8W2E0 4-coumarate:CoA ligase. Length = 536 Score = 389 bits (1000), Expect = e-107 Identities = 185/247 (74%), Positives = 218/247 (88%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 +SP + DL+S+RM+ SG AP+GK+L+D AK P A LGQGYGMTEAGPVLAMCLAFA Sbjct: 287 RSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFA 346 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++K G+CGTVVRNAE+KIVDP+T ASL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 347 KEPFDIKPGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRT 406 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALLI HPEI DAAVV Sbjct: 407 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVG 466 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF ++SE S+ +E+EIKQ+++K+V+FYKRI RVFF ++IPK PSGKILR Sbjct: 467 MKDEDAGEVPVAFAVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILR 526 Query: 727 KDLRARL 747 K+L+ +L Sbjct: 527 KNLKEKL 533 >sptr|Q94G19|Q94G19 4-coumarate:CoA ligase 3. Length = 540 Score = 389 bits (1000), Expect = e-107 Identities = 185/247 (74%), Positives = 217/247 (87%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP DL+S+RM+ SG AP+GK+L+D AK P A LGQGYGMTEAGPVLAMCLAFA Sbjct: 291 KSPDFDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFA 350 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++K G+CGTVVRNAE+KIVDP+T ASL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 351 KEPFDIKPGACGTVVRNAEMKIVDPETGASLRRNQPGEICIRGDQIMKGYLNDPEATSRT 410 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV PAELEALL+ HP+I DAAVV Sbjct: 411 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLAHPQISDAAVVG 470 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 MKDE AGEVPVAF+++SE S+ +E+EIKQ+++K+V+FYKRI RVFF ++IPK PSGKILR Sbjct: 471 MKDEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILR 530 Query: 727 KDLRARL 747 K+LR L Sbjct: 531 KNLRETL 537 >sptr|O81139|O81139 4-coumarate:CoA ligase (EC 6.2.1.12). Length = 535 Score = 388 bits (996), Expect = e-107 Identities = 183/247 (74%), Positives = 217/247 (87%) Frame = +1 Query: 7 KSPRVGAADLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFA 186 KSP + DL+S+RM+ SG AP+GK+L+D AK P A LGQGYGMTEAGPVLAMCLAFA Sbjct: 286 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFA 345 Query: 187 KEPFEVKSGSCGTVVRNAELKIVDPDTSASLGRNQPGEICIRGEQIMKGYLNDPEATKNT 366 KEPF++K G+CGTVVRNAE+KIVDP+T SL RNQPGEICIRG+QIMKGYLNDPEAT T Sbjct: 346 KEPFDIKPGACGTVVRNAEMKIVDPETGVSLPRNQPGEICIRGDQIMKGYLNDPEATSRT 405 Query: 367 IDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVS 546 IDK+GWLHTGDIGY+DDDDE+FIVDRLKE+IKYKGFQV P ELEALLI HPEI DAAVV Sbjct: 406 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPTELEALLIAHPEISDAAVVG 465 Query: 547 MKDEVAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILR 726 +KDE AGEVPVAF+++SE S+ +E+EIKQ+++K+V+FYKRI RVFF ++IPK PSGKILR Sbjct: 466 LKDEDAGEVPVAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILR 525 Query: 727 KDLRARL 747 K+L+ +L Sbjct: 526 KNLKEKL 532 Database: /db/trembl-ebi/tmp/swall Posted date: Jun 27, 2003 8:33 PM Number of letters in database: 376,898,118 Number of sequences in database: 1,175,531 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 682,745,731 Number of Sequences: 1175531 Number of extensions: 14594787 Number of successful extensions: 50239 Number of sequences better than 10.0: 30 Number of HSP's better than 10.0 without gapping: 45713 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 48300 length of database: 376,898,118 effective HSP length: 123 effective length of database: 232,307,805 effective search space used: 55753873200 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
S1: 41 (21.7 bits)